Abstract
A total of 1887 bovine sera positive to the Rose Bengal plate test were subjected to other serological tests for bovine brucellosis: the complement fixation test using warm fixation (CFTW),the serum agglutination test (SAT) and the radioimmunoassay (RIA). The SAT was generally much less sensitive than the CFTW. Many sera, however, gave positive reactions in the SAT but no reaction in the CFTW or the RIA. These SAT reactions were attributed to IgM antibody. Comparison between the results of the CFTW and the RIA led to the conclusion that 200 ng could be used as a minimum diagnostic reaction in the RIA.
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Selected References
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- Allan G. S., Chappel R. J., Williamson P., McNaught D. J. A quantitative comparison of the sensitivity of serological test for bovine brucellosis to different antibody classes. J Hyg (Lond) 1976 Apr;76(2):287–298. doi: 10.1017/s0022172400055182. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Alton G. G., Maw J., Rogerson B. A., McPherson G. G. The serological diagnosis of bovine brucellosis: an evaluation of the complement fixation, serum agglutination and rose bengal tests. Aust Vet J. 1975 Feb;51(2):57–63. doi: 10.1111/j.1751-0813.1975.tb09404.x. [DOI] [PubMed] [Google Scholar]
- Chappel R. J., McNaught D. J., Bourke J. A., Allan G. S. The diagnostic efficiency of some serological tests for bovine brucellosis. J Hyg (Lond) 1978 Jun;80(3):373–384. doi: 10.1017/s0022172400024827. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chappel R. J., Williamson P., McNaught D. J., Dalling M. J., Allan G. S. Radioimmunoassay for antibodies against Brucella abortus: a new serological test for bovine brucellosis. J Hyg (Lond) 1976 Dec;77(3):369–376. doi: 10.1017/s0022172400055741. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elberg S. S. Immunity to brucella infection. Medicine (Baltimore) 1973 Jul;52(4):339–356. doi: 10.1097/00005792-197307000-00013. [DOI] [PubMed] [Google Scholar]
- Morgan W. J., MacKinnon D. J., Lawson J. R., Cullen G. A. The rose bengal plate agglutination test in the diagnosis of brucellosis. Vet Rec. 1969 Dec 6;85(23):636–641. doi: 10.1136/vr.85.23.636. [DOI] [PubMed] [Google Scholar]