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. 1977 Apr;78(2):243–251. doi: 10.1017/s0022172400056138

Experimental transplacental transmission of porcine cytomegalovirus.

N Edington, R G Watt, W Plowright
PMCID: PMC2129840  PMID: 191522

Abstract

Six serologically negative sows were infected by intranasal instillation of porcine cytomegalovirus (PCMV) between 31 and 85 days of pregnacy. Four sows showed an afebrile anorexia and lethargy 14-25 days after infection and all 6 developed significant increases in indirect immunofluorescent (IIF) antibody titres within 35 days. Virus was recovered from nasal and/or cervical swabs from 2 sows during life and from lung macrophage cultures after death. At term the sows were killed and their fetuses harvested by caesarean section. The number of mummified and stillborn fetuses increased from 4/63 in 6 previous litters to 18/60 in the 6 present litters. Nine of 43 fetuses born alive were reared in isolators for up to 6 weeks but the majority were killed for examination on the day of birth. Virus was isolated from 16 piglets from 4 of the 6 litters examined; it was isolated most frequently from lungs and liver but also from spleen, kidney, brain and nasal mucosa. Unsuckled day-old pigs had insignificant IIF titres, irrespective of whether they were excreting virus or not. The 5 congenital excretors which were reared all died within 7 days but no death occurred among their 4 litter-mates. Post-natal infection of 2 of these piglets reared in contact with congenitally infected pigs was suggested by the recovery of virus from nasal swabs 17 and 27 days after birth and the subsequent rise in IIF titre to 1/256 by day 42.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann P. A., Sheffy B. E., Vauhan J. T. Experimental in utero infection of fetal pigs with a porcine parvovirus. Infect Immun. 1975 Sep;12(3):455–460. doi: 10.1128/iai.12.3.455-460.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Binns R. M. Bone marrow and lymphoid cell injection of the pig foetus resulting in transplantation tolerance or immunity, and immunoglobulin production. Nature. 1967 Apr 8;214(5084):179–180. doi: 10.1038/214179a0. [DOI] [PubMed] [Google Scholar]
  3. CORNER A. H., MITCHELL D., JULIAN R. J., MEADS E. B. A GENERALIZED DISEASE IN PIGLETS ASSOCIATED WITH THE PRESENCE OF CYTOMEGALIC INCLUSIONS. J Comp Pathol. 1964 Apr;74:192–199. doi: 10.1016/s0368-1742(64)80024-2. [DOI] [PubMed] [Google Scholar]
  4. Dunne H. W., Wang J. T., Clark C. D., Hokanson J. F., Morimoto T., Bubash G. R. The effects of in utero viral infection on embryonic, fetal, and neonatal survival: a comparison of SMEDI (porcine picorna) viruses with hog cholera vaccinal virus. Can J Comp Med. 1969 Oct;33(4):244–252. [PMC free article] [PubMed] [Google Scholar]
  5. Edington N., Plowright W., Watt R. G. Generalized porcine cytomegalic inclusion disease: distribution of cytomegalic cells and virus. J Comp Pathol. 1976 Apr;86(2):191–202. doi: 10.1016/0021-9975(76)90043-8. [DOI] [PubMed] [Google Scholar]
  6. Edington N., Watt R. G., Plowright W. Cytomegalovirus excretion in gnotobiotic pigs. J Hyg (Lond) 1976 Oct;77(2):283–290. doi: 10.1017/s0022172400024724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jamrichová O., Sokol A., Sevcík A. Distribution of attenuated vaccine strains of pseudorabies virus in intraperitoneally infected swine foetuses. Acta Virol. 1971 Jul;15(4):286–292. [PubMed] [Google Scholar]
  8. Levinsohn E. M., Foy H. M., Kenny G. E., Wentworth B. B., Grayston J. T. Isolation of cytomegalovirus from a cohort of 100 infants throughout the first year of life. Proc Soc Exp Biol Med. 1969 Dec;132(3):957–962. doi: 10.3181/00379727-132-34345. [DOI] [PubMed] [Google Scholar]
  9. Montgomery R., Youngblood L., Medearis D. N., Jr Recovery of cytomegalovirus from the cervix in pregnancy. Pediatrics. 1972 Apr;49(4):524–531. [PubMed] [Google Scholar]
  10. Numazaki Y., Yano N., Morizuka T., Takai S., Ishida N. Primary infection with human cytomegalovirus: virus isolation from healthy infants and pregnant women. Am J Epidemiol. 1970 Apr;91(4):410–417. doi: 10.1093/oxfordjournals.aje.a121151. [DOI] [PubMed] [Google Scholar]
  11. Plowright W., Edington N., Watt R. G. The behaviour of porcine cytomegalovirus in commercial pig herds. J Hyg (Lond) 1976 Feb;76(1):125–135. doi: 10.1017/s0022172400055017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wrathall A. E., Bailey J., Hebert C. N. A radiographic study of development of the appendicular skeleton in the fetal pig. Res Vet Sci. 1974 Sep;17(2):154–168. [PubMed] [Google Scholar]

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