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. 1979 Jun;82(3):407–418. doi: 10.1017/s0022172400053936

Differentiation of Mycoplasma mycoides subsp. mycoides from certain closely related caprine mycoplasmas by mycoplasmaemia and cross-protection tests in mice.

J M Hooker, G R Smith, R A Milligan
PMCID: PMC2130076  PMID: 376695

Abstract

In recent years, mycoplasma taxonomists have found that numerous mycoplasma strains from goats are serologically indistinguishable from Mycoplasma mycoides subsp. mycoides, the causative agent of contagious bovine pleuropneumonia (CBPP), by routinely used tests, e.g. the metabolism- and growth-inhibition tests. As a result, such organisms are now openly referred to as M. mycoides subsp. mycoides. Seven of these so-called M. mycoides subsp. mycoides strains from goats were compared with two strains of M. mycoides subsp. mycoides from CBPP, and with one strain of M. mycoides subsp. capri, by means of two in-vivo tests, namely, (1) a test of the ability of each strain, injected intraperitoneally into mice, to produce mycoplasmaemia, and (2) a cross-protection test in mice. Of the seven strains, only one ('O goat') was indistinguishable from genuine M. mycoides subsp. mycoides; it also had small colonies resembling those of genuine M. mycoides subsp. mycoides. The other six were easily distinguished from genuine M. mycoides subsp. mycoides, and they produced large colonies. These six strains and others like them should no longer be given a name that fails to distinguish them from the causative agent of CBPP. Cross-protection tests showed that the seven goat strains referred to above differed from M. mycoides subsp. capri.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cottew G. S., Watson W. A., Erdag O., Arisoy F. Mycoplasmas of caprine pleuropneumonia in turkey and their relationship to other mycoplasmas of goats and M. mycoides var. mycoides. J Comp Pathol. 1969 Oct;79(4):541–551. doi: 10.1016/0021-9975(69)90075-9. [DOI] [PubMed] [Google Scholar]
  2. Dyson D. A., Smith G. R. Contagious bovine pleuropneumonia: mouse-protective antibody in the sera of infected and vaccinated cattle. Res Vet Sci. 1975 Jul;19(1):8–16. [PubMed] [Google Scholar]
  3. Dyson D. A., Smith G. R. Virulence of established vaccine strains and artificially passaged field strain of Mycoplasma mycoides subsp mycoides. Res Vet Sci. 1976 Mar;20(2):185–190. [PubMed] [Google Scholar]
  4. Erno H., Freundt E. A., Krogsgaard-Jensen A., Rosendal S. The identification of an organism isolated from maned sheep as Mycoplasma mycoides subsp. mycoides. Acta Vet Scand. 1972;13(2):263–265. doi: 10.1186/BF03548581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gee R. W. Caprine mycoplasmosis. Aust Vet J. 1977 Jun;53(6):298–299. doi: 10.1111/j.1751-0813.1977.tb00233.x. [DOI] [PubMed] [Google Scholar]
  6. Hudson J. R., Cottew G. S., Adler H. E. Diseases of goats caused by mycoplasma: a review of the subject with some new findings. Ann N Y Acad Sci. 1967 Jul 28;143(1):287–297. doi: 10.1111/j.1749-6632.1967.tb27668.x. [DOI] [PubMed] [Google Scholar]
  7. Jonas A. M., Barber T. L. Mycoplasma mycoides var, capri isolated from a goat in Connecticut. J Infect Dis. 1969 Feb;119(2):126–131. doi: 10.1093/infdis/119.2.126. [DOI] [PubMed] [Google Scholar]
  8. MacOwan K. J. A Mycoplasma from chronic caprine pleuropneumonia in Kenya. Trop Anim Health Prod. 1976 Feb;8(1):28–36. doi: 10.1007/BF02383362. [DOI] [PubMed] [Google Scholar]
  9. Nasri M E. l. Mycoplasma from contagious caprine pleuropneumonia. Ann N Y Acad Sci. 1967 Jul 28;143(1):298–304. doi: 10.1111/j.1749-6632.1967.tb27669.x. [DOI] [PubMed] [Google Scholar]
  10. Smith G. R. A study of Mycoplasma mycoides var. mycoides and Mycoplasma mycoides var. capri by cross-protection tests in mice. J Comp Pathol. 1969 Apr;79(2):261–265. doi: 10.1016/0021-9975(69)90015-2. [DOI] [PubMed] [Google Scholar]
  11. Smith G. R. Effect of route of vaccination on the immune response of mice to a single dose of heat-killed Mycoplasma mycoides var. mycoides. J Comp Pathol. 1969 Apr;79(2):255–260. doi: 10.1016/0021-9975(69)90014-0. [DOI] [PubMed] [Google Scholar]
  12. Smith G. R. Experimental infection of mice with Mycoplasma mycoides var. capri. J Comp Pathol. 1967 Jan;77(1):21–27. doi: 10.1016/s0021-9975(67)80003-3. [DOI] [PubMed] [Google Scholar]
  13. Smith G. R. Factors affecting bacteriaemia in mice inoculated with Mycoplasma mycoides var. mycoides. J Comp Pathol. 1968 Jul;78(3):267–274. doi: 10.1016/0021-9975(68)90002-9. [DOI] [PubMed] [Google Scholar]
  14. Smith G. R. The use of mice in research on contagious bovine pleuropneumonia. Trop Anim Health Prod. 1971 Aug;3(3):169–172. doi: 10.1007/BF02360747. [DOI] [PubMed] [Google Scholar]

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