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. 1990 Sep;172(9):5097–5102. doi: 10.1128/jb.172.9.5097-5102.1990

The native form of FtsA, a septal protein of Escherichia coli, is located in the cytoplasmic membrane.

J Pla 1, A Dopazo 1, M Vicente 1
PMCID: PMC213167  PMID: 2203741

Abstract

Antisera able to recognize FtsA, one of the septal proteins of Escherichia coli, have been obtained and used to show that native FtsA, when expressed at levels ranging from physiological to induced from lambda pR, is located in the inner membrane. Experiments of trypsin accessibility to FtsA in membranes, spheroplasts, and vesicles indicated that FtsA is located such that it faces the cytoplasm. This location is consistent with current knowledge about the participation of FtsA in a molecular complex active in cell division called septator.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldea M., Garrido T., Hernández-Chico C., Vicente M., Kushner S. R. Induction of a growth-phase-dependent promoter triggers transcription of bolA, an Escherichia coli morphogene. EMBO J. 1989 Dec 1;8(12):3923–3931. doi: 10.1002/j.1460-2075.1989.tb08573.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ayala J. A., Plá J., Desviat L. R., de Pedro M. A. A lacZ-pbpB gene fusion coding for an inducible hybrid protein that recognizes localized sites in the inner membrane of Escherichia coli. J Bacteriol. 1988 Aug;170(8):3333–3341. doi: 10.1128/jb.170.8.3333-3341.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burdett I. D., Murray R. G. Septum formation in Escherichia coli: characterization of septal structure and the effects of antibiotics on cell division. J Bacteriol. 1974 Jul;119(1):303–324. doi: 10.1128/jb.119.1.303-324.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chon Y., Gayda R. Studies with FtsA-LacZ protein fusions reveal FtsA located inner-outer membrane junctions. Biochem Biophys Res Commun. 1988 May 16;152(3):1023–1030. doi: 10.1016/s0006-291x(88)80386-3. [DOI] [PubMed] [Google Scholar]
  6. Descoteaux A., Drapeau G. R. Regulation of cell division in Escherichia coli K-12: probable interactions among proteins FtsQ, FtsA, and FtsZ. J Bacteriol. 1987 May;169(5):1938–1942. doi: 10.1128/jb.169.5.1938-1942.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Donachie W. D., Begg K. J., Lutkenhaus J. F., Salmond G. P., Martinez-Salas E., Vincente M. Role of the ftsA gene product in control of Escherichia coli cell division. J Bacteriol. 1979 Nov;140(2):388–394. doi: 10.1128/jb.140.2.388-394.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Lutkenhaus J. F., Moore B. A., Masters M., Donachie W. D. Individual proteins are synthesized continuously throughout the Escherichia coli cell cycle. J Bacteriol. 1979 May;138(2):352–360. doi: 10.1128/jb.138.2.352-360.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Martínez-Salas E., Vicente M. Amber mutation affecting the length of Escherichia coli cells. J Bacteriol. 1980 Nov;144(2):532–541. doi: 10.1128/jb.144.2.532-541.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Osborn M. J., Gander J. E., Parisi E., Carson J. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J Biol Chem. 1972 Jun 25;247(12):3962–3972. [PubMed] [Google Scholar]
  13. Robinson A. C., Begg K. J., Sweeney J., Condie A., Donachie W. D. Mapping and characterization of mutants of the Escherichia coli cell division gene, ftsA. Mol Microbiol. 1988 Sep;2(5):581–588. doi: 10.1111/j.1365-2958.1988.tb00066.x. [DOI] [PubMed] [Google Scholar]
  14. Robinson A. C., Kenan D. J., Hatfull G. F., Sullivan N. F., Spiegelberg R., Donachie W. D. DNA sequence and transcriptional organization of essential cell division genes ftsQ and ftsA of Escherichia coli: evidence for overlapping transcriptional units. J Bacteriol. 1984 Nov;160(2):546–555. doi: 10.1128/jb.160.2.546-555.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tormo A., Ayala J. A., de Pedro M. A., Aldea M., Vicente M. Interaction of FtsA and PBP3 proteins in the Escherichia coli septum. J Bacteriol. 1986 Jun;166(3):985–992. doi: 10.1128/jb.166.3.985-992.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tormo A., Martínez-Salas E., Vicente M. Involvement of the ftsA gene product in late stages of the Escherichia coli cell cycle. J Bacteriol. 1980 Feb;141(2):806–813. doi: 10.1128/jb.141.2.806-813.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tormo A., Vicente M. The ftsA gene product participates in formation of the Escherichia coli septum structure. J Bacteriol. 1984 Mar;157(3):779–784. doi: 10.1128/jb.157.3.779-784.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ward J. E., Jr, Lutkenhaus J. Overproduction of FtsZ induces minicell formation in E. coli. Cell. 1985 Oct;42(3):941–949. doi: 10.1016/0092-8674(85)90290-9. [DOI] [PubMed] [Google Scholar]
  20. Yi Q. M., Rockenbach S., Ward J. E., Jr, Lutkenhaus J. Structure and expression of the cell division genes ftsQ, ftsA and ftsZ. J Mol Biol. 1985 Aug 5;184(3):399–412. doi: 10.1016/0022-2836(85)90290-6. [DOI] [PubMed] [Google Scholar]

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