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. 1990 Sep;172(9):5286–5292. doi: 10.1128/jb.172.9.5286-5292.1990

Relationship between utilization of proline and proline-containing peptides and growth of Lactococcus lactis.

E J Smid 1, W N Konings 1
PMCID: PMC213191  PMID: 2118509

Abstract

Proline, which is the most abundant residue in beta-casein, stimulates growth of Lactococcus lactis in a proline-requiring strain (Lactococcus lactis subsp. cremoris Wg2) and in a proline-prototrophic strain (Lactococcus lactis subsp. lactis ML3). Both strains lack a proline-specific uptake system, and free proline can enter the cell only by passive diffusion across the cytoplasmic membrane. On the other hand, lactococci can actively take up proline-containing peptides via the lactococcal di- and tripeptide transport system, and these peptides are the major source of proline. Consequently, lactococcal growth on amino acid-based media is highly stimulated by the addition of proline-containing di- and tripeptides. Growth of L. lactis subsp. lactis ML3 on chemically defined media supplemented with casein does not appear proline limited. Addition of dipeptides (including proline-containing peptides) severely inhibits growth on a casein-containing medium, which indicates that the specific growth rate is determined by the balanced supply of different di- or tripeptides which compete for the same di- and tripeptide transport system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bosman B. W., Tan P. S., Konings W. N. Purification and Characterization of a Tripeptidase from Lactococcus lactis subsp. cremoris Wg2. Appl Environ Microbiol. 1990 Jun;56(6):1839–1843. doi: 10.1128/aem.56.6.1839-1843.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Konings W. N., Poolman B., Driessen A. J. Bioenergetics and solute transport in lactococci. Crit Rev Microbiol. 1989;16(6):419–476. doi: 10.3109/10408418909104474. [DOI] [PubMed] [Google Scholar]
  3. Poolman B., Konings W. N. Relation of growth of Streptococcus lactis and Streptococcus cremoris to amino acid transport. J Bacteriol. 1988 Feb;170(2):700–707. doi: 10.1128/jb.170.2.700-707.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Poolman B., Smid E. J., Veldkamp H., Konings W. N. Bioenergetic consequences of lactose starvation for continuously cultured Streptococcus cremoris. J Bacteriol. 1987 Apr;169(4):1460–1468. doi: 10.1128/jb.169.4.1460-1468.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Smid E. J., Driessen A. J., Konings W. N. Mechanism and energetics of dipeptide transport in membrane vesicles of Lactococcus lactis. J Bacteriol. 1989 Jan;171(1):292–298. doi: 10.1128/jb.171.1.292-298.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Smid E. J., Plapp R., Konings W. N. Peptide uptake is essential for growth of Lactococcus lactis on the milk protein casein. J Bacteriol. 1989 Nov;171(11):6135–6140. doi: 10.1128/jb.171.11.6135-6140.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Tan P. S., Konings W. N. Purification and Characterization of an Aminopeptidase from Lactococcus lactis subsp. cremoris Wg2. Appl Environ Microbiol. 1990 Feb;56(2):526–532. doi: 10.1128/aem.56.2.526-532.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Tapuhi Y., Schmidt D. E., Lindner W., Karger B. L. Dansylation of amino acids for high-performance liquid chromatography analysis. Anal Biochem. 1981 Jul 15;115(1):123–129. doi: 10.1016/0003-2697(81)90534-0. [DOI] [PubMed] [Google Scholar]
  9. Wiedmeier V. T., Porterfield S. P., Hendrich C. E. Quantitation of Dns-amino acids from body tissues and fluids using high-performance liquid chromatography. J Chromatogr. 1982 Sep 10;231(2):410–417. doi: 10.1016/s0378-4347(00)81865-4. [DOI] [PubMed] [Google Scholar]
  10. ten Brink B., Otto R., Hansen U. P., Konings W. N. Energy recycling by lactate efflux in growing and nongrowing cells of Streptococcus cremoris. J Bacteriol. 1985 Apr;162(1):383–390. doi: 10.1128/jb.162.1.383-390.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. van Boven A., Tan P. S. T., Konings W. N. Purification and Characterization of a Dipeptidase from Streptococcus cremoris Wg2. Appl Environ Microbiol. 1988 Jan;54(1):43–49. doi: 10.1128/aem.54.1.43-49.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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