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. 1990 Sep;172(9):5482–5485. doi: 10.1128/jb.172.9.5482-5485.1990

The Bacillus subtilis outB gene is highly homologous to an Escherichia coli ntr-like gene.

A M Albertini 1, A Galizzi 1
PMCID: PMC213216  PMID: 2118513

Abstract

The Bacillus subtilis outB gene was found to have strong similarities to an Escherichia coli gene complementing ntr-like mutations in Rhodobacter capsulatus. The deduced gene products had 52% identical amino acids (65% similar residues). The phenotype of strains affected in the OutB function indicates that this B. subtilis gene may be involved in nitrogen utilization.

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Selected References

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  1. Albertini A. M., Caramori T., Henner D., Ferrari E., Galizzi A. Nucleotide sequence of the outB locus of Bacillus subtilis and regulation of its expression. J Bacteriol. 1987 Apr;169(4):1480–1484. doi: 10.1128/jb.169.4.1480-1484.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Albertini A. M., Caramori T., Scoffone F., Galizzi A. The outB gene of Bacillus subtilis regulates its own transcription. Genes Dev. 1988 Nov;2(11):1381–1388. doi: 10.1101/gad.2.11.1381. [DOI] [PubMed] [Google Scholar]
  3. Albertini A. M., Galizzi A. Mutant of Bacillus subtilis with a temperature-sensitive lesion in ribonucleic acid synthesis during germination. J Bacteriol. 1975 Oct;124(1):14–25. doi: 10.1128/jb.124.1.14-25.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Allibert P., Willison J. C., Vignais P. M. Complementation of nitrogen-regulatory (ntr-like) mutations in Rhodobacter capsulatus by an Escherichia coli gene: cloning and sequencing of the gene and characterization of the gene product. J Bacteriol. 1987 Jan;169(1):260–271. doi: 10.1128/jb.169.1.260-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bohannon D. E., Sonenshein A. L. Positive regulation of glutamate biosynthesis in Bacillus subtilis. J Bacteriol. 1989 Sep;171(9):4718–4727. doi: 10.1128/jb.171.9.4718-4727.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dean D. R., Hoch J. A., Aronson A. I. Alteration of the Bacillus subtilis glutamine synthetase results in overproduction of the enzyme. J Bacteriol. 1977 Sep;131(3):981–987. doi: 10.1128/jb.131.3.981-987.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fisher S. H., Rosenkrantz M. S., Sonenshein A. L. Glutamine synthetase gene of Bacillus subtilis. Gene. 1984 Dec;32(3):427–438. doi: 10.1016/0378-1119(84)90018-0. [DOI] [PubMed] [Google Scholar]
  8. Fisher S. H., Sonenshein A. L. Glutamine-requiring mutants of Bacillus subtilis. Biochem Biophys Res Commun. 1977 Dec 7;79(3):987–995. doi: 10.1016/0006-291x(77)91207-4. [DOI] [PubMed] [Google Scholar]
  9. Gardner A. L., Aronson A. I. Expression of the Bacillus subtilis glutamine synthetase gene in Escherichia coli. J Bacteriol. 1984 Jun;158(3):967–971. doi: 10.1128/jb.158.3.967-971.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Henkin T. M., Moon S. H., Mattheakis L. C., Nomura M. Cloning and analysis of the spc ribosomal protein operon of Bacillus subtilis: comparison with the spc operon of Escherichia coli. Nucleic Acids Res. 1989 Sep 25;17(18):7469–7486. doi: 10.1093/nar/17.18.7469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Perego M., Hoch J. A. Sequence analysis and regulation of the hpr locus, a regulatory gene for protease production and sporulation in Bacillus subtilis. J Bacteriol. 1988 Jun;170(6):2560–2567. doi: 10.1128/jb.170.6.2560-2567.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schreier H. J., Brown S. W., Hirschi K. D., Nomellini J. F., Sonenshein A. L. Regulation of Bacillus subtilis glutamine synthetase gene expression by the product of the glnR gene. J Mol Biol. 1989 Nov 5;210(1):51–63. doi: 10.1016/0022-2836(89)90290-8. [DOI] [PubMed] [Google Scholar]
  13. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Strauch M. A., Aronson A. I., Brown S. W., Schreier H. J., Sonenhein A. L. Sequence of the Bacillus subtilis glutamine synthetase gene region. Gene. 1988 Nov 30;71(2):257–265. doi: 10.1016/0378-1119(88)90042-x. [DOI] [PubMed] [Google Scholar]

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