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. 1990 Aug;172(8):4705–4707. doi: 10.1128/jb.172.8.4705-4707.1990

Genome size of Mycoplasma genitalium.

C J Su 1, J B Baseman 1
PMCID: PMC213309  PMID: 2115876

Abstract

The genome size of Mycoplasma genitalium was determined by using restriction enzymes that infrequently cut its DNA. The calculated value of 577 to 590 kilobases is one-fourth smaller than the genome of Mycoplasma pneumoniae, which is considered among the smallest genomes of self-replicating organisms.

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Selected References

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  1. Baseman J. B., Cole R. M., Krause D. C., Leith D. K. Molecular basis for cytadsorption of Mycoplasma pneumoniae. J Bacteriol. 1982 Sep;151(3):1514–1522. doi: 10.1128/jb.151.3.1514-1522.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baseman J. B., Dallo S. F., Tully J. G., Rose D. L. Isolation and characterization of Mycoplasma genitalium strains from the human respiratory tract. J Clin Microbiol. 1988 Nov;26(11):2266–2269. doi: 10.1128/jcm.26.11.2266-2269.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baseman J. B., Daly K. L., Trevino L. B., Drouillard D. L. Distinctions among pathogenic human mycoplasmas. Isr J Med Sci. 1984 Sep;20(9):866–869. [PubMed] [Google Scholar]
  4. Carle G. F., Olson M. V. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. Nucleic Acids Res. 1984 Jul 25;12(14):5647–5664. doi: 10.1093/nar/12.14.5647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dallo S. F., Chavoya A., Su C. J., Baseman J. B. DNA and protein sequence homologies between the adhesins of Mycoplasma genitalium and Mycoplasma pneumoniae. Infect Immun. 1989 Apr;57(4):1059–1065. doi: 10.1128/iai.57.4.1059-1065.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dallo S. F., Horton J. R., Su C. J., Baseman J. B. Homologous regions shared by adhesin genes of Mycoplasma pneumoniae and Mycoplasma genitalium. Microb Pathog. 1989 Jan;6(1):69–73. doi: 10.1016/0882-4010(89)90009-0. [DOI] [PubMed] [Google Scholar]
  7. Feldner J., Göbel U., Bredt W. Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody. Nature. 1982 Aug 19;298(5876):765–767. doi: 10.1038/298765a0. [DOI] [PubMed] [Google Scholar]
  8. Hu P. C., Cole R. M., Huang Y. S., Graham J. A., Gardner D. E., Collier A. M., Clyde W. A., Jr Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle. Science. 1982 Apr 16;216(4543):313–315. doi: 10.1126/science.6801766. [DOI] [PubMed] [Google Scholar]
  9. Hu P. C., Schaper U., Collier A. M., Clyde W. A., Jr, Horikawa M., Huang Y. S., Barile M. F. A Mycoplasma genitalium protein resembling the Mycoplasma pneumoniae attachment protein. Infect Immun. 1987 May;55(5):1126–1131. doi: 10.1128/iai.55.5.1126-1131.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Inamine J. M., Denny T. P., Loechel S., Schaper U., Huang C. H., Bott K. F., Hu P. C. Nucleotide sequence of the P1 attachment-protein gene of Mycoplasma pneumoniae. Gene. 1988 Apr 29;64(2):217–229. doi: 10.1016/0378-1119(88)90337-x. [DOI] [PubMed] [Google Scholar]
  11. Jackson D. A., Cook P. R. A general method for preparing chromatin containing intact DNA. EMBO J. 1985 Apr;4(4):913–918. doi: 10.1002/j.1460-2075.1985.tb03718.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lind K., Lindhardt B. O., Schütten H. J., Blom J., Christiansen C. Serological cross-reactions between Mycoplasma genitalium and Mycoplasma pneumoniae. J Clin Microbiol. 1984 Dec;20(6):1036–1043. doi: 10.1128/jcm.20.6.1036-1043.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morrison-Plummer J., Lazzell A., Baseman J. B. Shared epitopes between Mycoplasma pneumoniae major adhesin protein P1 and a 140-kilodalton protein of Mycoplasma genitalium. Infect Immun. 1987 Jan;55(1):49–56. doi: 10.1128/iai.55.1.49-56.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Razin S. Molecular biology and genetics of mycoplasmas (Mollicutes). Microbiol Rev. 1985 Dec;49(4):419–455. doi: 10.1128/mr.49.4.419-455.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Su C. J., Chavoya A., Baseman J. B. Regions of Mycoplasma pneumoniae cytadhesin P1 structural gene exist as multiple copies. Infect Immun. 1988 Dec;56(12):3157–3161. doi: 10.1128/iai.56.12.3157-3161.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Su C. J., Chavoya A., Baseman J. B. Spontaneous mutation results in loss of the cytadhesin (P1) of Mycoplasma pneumoniae. Infect Immun. 1989 Oct;57(10):3237–3239. doi: 10.1128/iai.57.10.3237-3239.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Su C. J., Tryon V. V., Baseman J. B. Cloning and sequence analysis of cytadhesin P1 gene from Mycoplasma pneumoniae. Infect Immun. 1987 Dec;55(12):3023–3029. doi: 10.1128/iai.55.12.3023-3029.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tryon V. V., Baseman J. B. The acquisition of human lactoferrin by Mycoplasma pneumoniae. Microb Pathog. 1987 Dec;3(6):437–443. doi: 10.1016/0882-4010(87)90013-1. [DOI] [PubMed] [Google Scholar]
  19. Tully J. G., Rose D. L., Whitcomb R. F., Wenzel R. P. Enhanced isolation of Mycoplasma pneumoniae from throat washings with a newly-modified culture medium. J Infect Dis. 1979 Apr;139(4):478–482. doi: 10.1093/infdis/139.4.478. [DOI] [PubMed] [Google Scholar]
  20. Tully J. G., Taylor-Robinson D., Cole R. M., Rose D. L. A newly discovered mycoplasma in the human urogenital tract. Lancet. 1981 Jun 13;1(8233):1288–1291. doi: 10.1016/s0140-6736(81)92461-2. [DOI] [PubMed] [Google Scholar]
  21. Wenzel R., Herrmann R. Physical mapping of the Mycoplasma pneumoniae genome. Nucleic Acids Res. 1988 Sep 12;16(17):8323–8336. doi: 10.1093/nar/16.17.8323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wenzel R., Herrmann R. Repetitive DNA sequences in Mycoplasma pneumoniae. Nucleic Acids Res. 1988 Sep 12;16(17):8337–8350. doi: 10.1093/nar/16.17.8337. [DOI] [PMC free article] [PubMed] [Google Scholar]

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