Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1990 Jul;172(7):4127–4131. doi: 10.1128/jb.172.7.4127-4131.1990

Nucleotide sequence of the traI (helicase I) gene from the sex factor F.

H D Bradshaw Jr 1, B A Traxler 1, E G Minkley Jr 1, E W Nester 1, M P Gordon 1
PMCID: PMC213404  PMID: 2163400

Abstract

A 6.9-kilobase region of the Escherichia coli F plasmid containing the 3' half of the traD gene and the entire traI gene (encodes the TraI protein, DNA helicase I and TraI, a polypeptide arising from an internal in-frame translational start in traI) has been sequenced. A previously unidentified open reading frame (tentatively trbH) lies between traD and traI.

Full text

PDF
4127

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdel-Monem M., Hoffmann-Berling H. Enzymic unwinding of DNA. 1. Purification and characterization of a DNA-dependent ATPase from Escherichia coli. Eur J Biochem. 1976 Jun 1;65(2):431–440. doi: 10.1111/j.1432-1033.1976.tb10358.x. [DOI] [PubMed] [Google Scholar]
  2. Abdel-Monem M., Taucher-Scholz G., Klinkert M. Q. Identification of Escherichia coli DNA helicase I as the traI gene product of the F sex factor. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4659–4663. doi: 10.1073/pnas.80.15.4659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Biggin M. D., Gibson T. J., Hong G. F. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. doi: 10.1073/pnas.80.13.3963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Deininger P. L. Random subcloning of sonicated DNA: application to shotgun DNA sequence analysis. Anal Biochem. 1983 Feb 15;129(1):216–223. doi: 10.1016/0003-2697(83)90072-6. [DOI] [PubMed] [Google Scholar]
  5. Everett R., Willetts N. Characterisation of an in vivo system for nicking at the origin of conjugal DNA transfer of the sex factor F. J Mol Biol. 1980 Jan 15;136(2):129–150. doi: 10.1016/0022-2836(80)90309-5. [DOI] [PubMed] [Google Scholar]
  6. Gaffney D., Skurray R., Willetts N. Regulation of the F conjugation genes studied by hybridization and tra-lacZ fusion. J Mol Biol. 1983 Jul 25;168(1):103–122. doi: 10.1016/s0022-2836(83)80325-8. [DOI] [PubMed] [Google Scholar]
  7. Hunkapiller M. W., Hewick R. M., Dreyer W. J., Hood L. E. High-sensitivity sequencing with a gas-phase sequenator. Methods Enzymol. 1983;91:399–413. doi: 10.1016/s0076-6879(83)91038-8. [DOI] [PubMed] [Google Scholar]
  8. Ippen-Ihler K. A., Minkley E. G., Jr The conjugation system of F, the fertility factor of Escherichia coli. Annu Rev Genet. 1986;20:593–624. doi: 10.1146/annurev.ge.20.120186.003113. [DOI] [PubMed] [Google Scholar]
  9. Lahue E. E., Matson S. W. Escherichia coli DNA helicase I catalyzes a unidirectional and highly processive unwinding reaction. J Biol Chem. 1988 Mar 5;263(7):3208–3215. [PubMed] [Google Scholar]
  10. Lipman D. J., Pearson W. R. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. doi: 10.1126/science.2983426. [DOI] [PubMed] [Google Scholar]
  11. Manning P. A., Kusecek B., Morelli G., Fisseau C., Achtman M. Analysis of the promoter-distal region of the tra operon of the F sex factor of Escherichia coli K-12 encoded by EcoRI restriction fragments f17, f19, and f2. J Bacteriol. 1982 Apr;150(1):76–88. doi: 10.1128/jb.150.1.76-88.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Manning P. A., Morelli G., Fisseau C. RNA-polymerase binding sites within the tra region of the F factor of Escherichia coli K-12. Gene. 1984 Jan;27(1):121–123. doi: 10.1016/0378-1119(84)90245-2. [DOI] [PubMed] [Google Scholar]
  13. Moore D., Sowa B. A., Ippen-Ihler K. A new activity in the Ftra operon which is required for F-pilin synthesis. Mol Gen Genet. 1982;188(3):459–464. doi: 10.1007/BF00330049. [DOI] [PubMed] [Google Scholar]
  14. Mulligan M. E., McClure W. R. Analysis of the occurrence of promoter-sites in DNA. Nucleic Acids Res. 1986 Jan 10;14(1):109–126. doi: 10.1093/nar/14.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mullineaux P., Willetts N. Promoters in the transfer region of plasmid F. Basic Life Sci. 1985;30:605–614. doi: 10.1007/978-1-4613-2447-8_42. [DOI] [PubMed] [Google Scholar]
  16. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Traxler B. A., Minkley E. G., Jr Evidence that DNA helicase I and oriT site-specific nicking are both functions of the F TraI protein. J Mol Biol. 1988 Nov 5;204(1):205–209. doi: 10.1016/0022-2836(88)90609-2. [DOI] [PubMed] [Google Scholar]
  18. Traxler B. A., Minkley E. G., Jr Revised genetic map of the distal end of the F transfer operon: implications for DNA helicase I, nicking at oriT, and conjugal DNA transport. J Bacteriol. 1987 Jul;169(7):3251–3259. doi: 10.1128/jb.169.7.3251-3259.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Walker J. E., Saraste M., Runswick M. J., Gay N. J. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1982;1(8):945–951. doi: 10.1002/j.1460-2075.1982.tb01276.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Willetts N., Wilkins B. Processing of plasmid DNA during bacterial conjugation. Microbiol Rev. 1984 Mar;48(1):24–41. doi: 10.1128/mr.48.1.24-41.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES