Abstract
Antigenic variants of A/Texas/77 (H3N2) virus were selected in vitro using monoclonal antibody to virus haemagglutinin (HA). The antigenic variants and parental A/Texas/77 viruses were used to to evaluate the frequency of anti-HA antibodies in the sera of children and adults using single-radial-haemolysis (SRH) tests. Twenty to 41% of selected sera from adults, which contained antibody to the parental A/Texas/77 virus, failed to react with the different antigenic mutant viruses. A higher proportion of sera from children (37-58%) failed to react with the antigenic variants. Certain human sera and particularly those of children would appear to possess a more limited antibody repertoire to influenza HA, potentially allowing new antigenic variants to escape neutralization and spread in the community.
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- Dowdle W. R., Millar J. D., Schonberger L. B., Ennis F. A., LaMontagne J. R. Influenza immunization policies and practices in Japan. J Infect Dis. 1980 Feb;141(2):258–264. doi: 10.1093/infdis/141.2.258. [DOI] [PubMed] [Google Scholar]
- Gerhard W., Webster R. G. Antigenic drift in influenza A viruses. I. Selection and characterization of antigenic variants of A/PR/8/34 (HON1) influenza virus with monoclonal antibodies. J Exp Med. 1978 Aug 1;148(2):383–392. doi: 10.1084/jem.148.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haaheim L. R. Haemagglutination-inhibition antibodies in human sera to an antigenic mutant of influenza A/Texas/1/77 (H3N2) virus obtained in vitro. Acta Pathol Microbiol Scand B. 1980 Dec;88(6):351–353. doi: 10.1111/j.1699-0463.1980.tb02656.x. [DOI] [PubMed] [Google Scholar]
- Kilbourne E. D. Genetic dimorphism in influenza viruses: characterization of stably associated hemagglutinin mutants differing in antigenicity and biological properties. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6258–6262. doi: 10.1073/pnas.75.12.6258. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koprowski H., Gerhard W., Croce C. M. Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2985–2988. doi: 10.1073/pnas.74.7.2985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laver W. G., Air G. M., Webster R. G., Gerhard W., Ward C. W., Dopheide T. A. The mechanism of antigenic drift in influenza virus: sequence changes in the haemagglutinin of variants selected with monoclonal hybridoma antibodies. Philos Trans R Soc Lond B Biol Sci. 1980 Feb 25;288(1029):313–326. doi: 10.1098/rstb.1980.0007. [DOI] [PubMed] [Google Scholar]
- Laver W. G., Gerhard W., Webster R. G., Frankel M. E., Air G. M. Antigenic drift in type A influenza virus: peptide mapping and antigenic analysis of A/PR/8/34 (HON1) variants selected with monoclonal antibodies. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1425–1429. doi: 10.1073/pnas.76.3.1425. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oxford J. S., Haaheim L. R., Slepushkin A., Werner J., Kuwert E., Schild G. C. Strain specificity of serum antibody to the haemagglutinin of influenza A (H3N2) viruses in children following immunization or natural infection. J Hyg (Lond) 1981 Feb;86(1):17–26. doi: 10.1017/s0022172400068704. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schild G. C., Pereira M. S., Chakraverty P. Single-radial-hemolysis: a new method for the assay of antibody to influenza haemagglutinin. Applications for diagnosis and seroepidemiologic surveillance of influenza. Bull World Health Organ. 1975;52(1):43–50. [PMC free article] [PubMed] [Google Scholar]
- Yewdell J. W., Webster R. G., Gerhard W. U. Antigenic variation in three distinct determinants of an influenza type A haemagglutinin molecule. Nature. 1979 May 17;279(5710):246–248. doi: 10.1038/279246a0. [DOI] [PubMed] [Google Scholar]