Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1986 Nov;168(2):940–946. doi: 10.1128/jb.168.2.940-946.1986

recA (Srf) suppression of recF deficiency in the postreplication repair of UV-irradiated Escherichia coli K-12.

T C Wang, K C Smith
PMCID: PMC213575  PMID: 3023291

Abstract

The mechanism by which recA (Srf) mutations (recA2020 and recA801) suppress the deficiency in postreplication repair shown by recF mutants of Escherichia coli was studied in UV-irradiated uvrB and uvrA recB recC sbcB cells. The recA (Srf) mutations partially suppressed the UV radiation sensitivity of uvrB recF, uvrB recF recB, and uvrA recB recC sbcB recF cells, and they partially restored the ability of uvrB recF and uvrA recB recC sbcB recF cells to repair DNA daughter-strand gaps. In addition, the recA (Srf) mutations suppressed the recF deficiency in the repair of DNA double-strand breaks in UV-irradiated uvrA recB recC sbcB recF cells. The recA2020 and recA801 mutations do not appear to affect the synthesis of UV radiation-induced proteins, nor do they appear to produce an altered RecA protein, as detected by two-dimensional gel electrophoresis. These results are consistent with the suggestion (M. R. Volkert and M. A. Hartke, J. Bacteriol. 157:498-506, 1984) that the recA (Srf) mutations do not act by affecting the induction of SOS responses; rather, they allow the RecA protein to participate in the recF-dependent postreplication repair processes without the need of the RecF protein.

Full text

PDF
940

Images in this article

945Image
on p.945
945Image
on p.945

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYCE R. P., HOWARD-FLANDERS P. RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12. Proc Natl Acad Sci U S A. 1964 Feb;51:293–300. doi: 10.1073/pnas.51.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blanar M. A., Sandler S. J., Armengod M. E., Ream L. W., Clark A. J. Molecular analysis of the recF gene of Escherichia coli. Proc Natl Acad Sci U S A. 1984 Aug;81(15):4622–4626. doi: 10.1073/pnas.81.15.4622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Casaregola S., D'Ari R., Huisman O. Quantitative evaluation of recA gene expression in Escherichia coli. Mol Gen Genet. 1982;185(3):430–439. doi: 10.1007/BF00334135. [DOI] [PubMed] [Google Scholar]
  5. Clark A. J., Sandler S. J., Willis D. K., Chu C. C., Blanar M. A., Lovett S. T. Genes of the RecE and RecF pathways of conjugational recombination in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1984;49:453–462. doi: 10.1101/sqb.1984.049.01.051. [DOI] [PubMed] [Google Scholar]
  6. Clark A. J., Volkert M. R., Margossian L. J. A role for recF in repair of UV damage to DNA. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):887–892. doi: 10.1101/sqb.1979.043.01.096. [DOI] [PubMed] [Google Scholar]
  7. Finch P. W., Chambers P., Emmerson P. T. Identification of the Escherichia coli recN gene product as a major SOS protein. J Bacteriol. 1985 Nov;164(2):653–658. doi: 10.1128/jb.164.2.653-658.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ganesan A. K., Seawell P. C. The effect of lexA and recF mutations on post-replication repair and DNA synthesis in Escherichia coli K-12. Mol Gen Genet. 1975 Dec 1;141(3):189–205. doi: 10.1007/BF00341799. [DOI] [PubMed] [Google Scholar]
  9. Karu A. E., Belk E. D. Induction of E. coli recA protein via recBC and alternate pathways: quantitation by enzyme-linked immunosorbent assay (ELISA). Mol Gen Genet. 1982;185(2):275–282. doi: 10.1007/BF00330798. [DOI] [PubMed] [Google Scholar]
  10. Kato T. Effects of chloramphenicol and caffeine on postreplication repair in uvr A- umuC- und uvrA- recF- strains of Escherichia coli K-12. Mol Gen Genet. 1977 Nov 14;156(2):115–120. doi: 10.1007/BF00283483. [DOI] [PubMed] [Google Scholar]
  11. Laemmli U. K., Favre M. Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol. 1973 Nov 15;80(4):575–599. doi: 10.1016/0022-2836(73)90198-8. [DOI] [PubMed] [Google Scholar]
  12. Lloyd R. G., Buckman C. Identification and genetic analysis of sbcC mutations in commonly used recBC sbcB strains of Escherichia coli K-12. J Bacteriol. 1985 Nov;164(2):836–844. doi: 10.1128/jb.164.2.836-844.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McPartland A., Green L., Echols H. Control of recA gene RNA in E. coli: regulatory and signal genes. Cell. 1980 Jul;20(3):731–737. doi: 10.1016/0092-8674(80)90319-0. [DOI] [PubMed] [Google Scholar]
  14. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  15. Picksley S. M., Attfield P. V., Lloyd R. G. Repair of DNA double-strand breaks in Escherichia coli K12 requires a functional recN product. Mol Gen Genet. 1984;195(1-2):267–274. doi: 10.1007/BF00332758. [DOI] [PubMed] [Google Scholar]
  16. Picksley S. M., Lloyd R. G., Buckman C. Genetic analysis and regulation of inducible recombination in Escherichia coli K-12. Cold Spring Harb Symp Quant Biol. 1984;49:469–474. doi: 10.1101/sqb.1984.049.01.053. [DOI] [PubMed] [Google Scholar]
  17. Rothman R. H., Clark A. J. The dependence of postreplication repair on uvrB in a recF mutant of Escherichia coli K-12. Mol Gen Genet. 1977 Oct 24;155(3):279–286. doi: 10.1007/BF00272806. [DOI] [PubMed] [Google Scholar]
  18. Rupp W. D., Howard-Flanders P. Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation. J Mol Biol. 1968 Jan 28;31(2):291–304. doi: 10.1016/0022-2836(68)90445-2. [DOI] [PubMed] [Google Scholar]
  19. Rupp W. D., Wilde C. E., 3rd, Reno D. L., Howard-Flanders P. Exchanges between DNA strands in ultraviolet-irradiated Escherichia coli. J Mol Biol. 1971 Oct 14;61(1):25–44. doi: 10.1016/0022-2836(71)90204-x. [DOI] [PubMed] [Google Scholar]
  20. SETLOW R. B., CARRIER W. L. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM. Proc Natl Acad Sci U S A. 1964 Feb;51:226–231. doi: 10.1073/pnas.51.2.226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Salles B., Paoletti C. Control of UV induction of recA protein. Proc Natl Acad Sci U S A. 1983 Jan;80(1):65–69. doi: 10.1073/pnas.80.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sargentini N. J., Smith K. C. Characterization and quantitation of DNA strand breaks requiring recA-dependent repair in X-irradiated Escherichia coli. Radiat Res. 1986 Feb;105(2):180–186. [PubMed] [Google Scholar]
  23. Sargentini N. J., Smith K. C. Quantitation of the involvement of the recA, recB, recC, recF, recJ, recN, lexA, radA, radB, uvrD, and umuC genes in the repair of X-ray-induced DNA double-strand breaks in Escherichia coli. Radiat Res. 1986 Jul;107(1):58–72. [PubMed] [Google Scholar]
  24. Sargentini N. J., Smith K. C. Role of the radB gene in postreplication repair in UV-irradiated Escherichia coli uvrB. Mutat Res. 1986 Jul;166(1):17–22. doi: 10.1016/0167-8817(86)90036-2. [DOI] [PubMed] [Google Scholar]
  25. Smith K. C., Meun D. H. Repair of radiation-induced damage in Escherichia coli. I. Effect of rec mutations on post-replication repair of damage due to ultraviolet radiation. J Mol Biol. 1970 Aug;51(3):459–472. doi: 10.1016/0022-2836(70)90001-x. [DOI] [PubMed] [Google Scholar]
  26. Volkert M. R., Hartke M. A. Suppression of Escherichia coli recF mutations by recA-linked srfA mutations. J Bacteriol. 1984 Feb;157(2):498–506. doi: 10.1128/jb.157.2.498-506.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Volkert M. R., Margossian L. J., Clark A. J. Two-component suppression of recF143 by recA441 in Escherichia coli K-12. J Bacteriol. 1984 Nov;160(2):702–705. doi: 10.1128/jb.160.2.702-705.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Walker G. C. Inducible DNA repair systems. Annu Rev Biochem. 1985;54:425–457. doi: 10.1146/annurev.bi.54.070185.002233. [DOI] [PubMed] [Google Scholar]
  29. Wang T. C., Smith K. C. Effects of the ssb-1 and ssb-113 mutations on survival and DNA repair in UV-irradiated delta uvrB strains of Escherichia coli K-12. J Bacteriol. 1982 Jul;151(1):186–192. doi: 10.1128/jb.151.1.186-192.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wang T. C., Smith K. C. Mechanism of sbcB-suppression of the recBC-deficiency in postreplication repair in UV-irradiated Escherichia coli K-12. Mol Gen Genet. 1985;201(2):186–191. doi: 10.1007/BF00425658. [DOI] [PubMed] [Google Scholar]
  31. Wang T. C., Smith K. C. Mechanisms for recF-dependent and recB-dependent pathways of postreplication repair in UV-irradiated Escherichia coli uvrB. J Bacteriol. 1983 Dec;156(3):1093–1098. doi: 10.1128/jb.156.3.1093-1098.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wang T. C., Smith K. C. Postreplicational formation and repair of DNA double-strand breaks in UV-irradiated Escherichia coli uvrB cells. Mutat Res. 1986 Jan;165(1):39–44. doi: 10.1016/0167-8817(86)90007-6. [DOI] [PubMed] [Google Scholar]
  33. Wang T. V., Smith K. C. Effect of recB21, uvrD3, lexA101 and recF143 mutations on ultraviolet radiation sensitivity and genetic recombination in delta uvrB strains of Escherichia coli K-12. Mol Gen Genet. 1981;183(1):37–44. doi: 10.1007/BF00270135. [DOI] [PubMed] [Google Scholar]
  34. Wang T. V., Smith K. C. Rich growth medium enhances ultraviolet radiation sensitivity and inhibits cell division in ssb mutants of Escherichia coli K-12. Photochem Photobiol. 1984 Jun;39(6):793–797. doi: 10.1111/j.1751-1097.1984.tb08861.x. [DOI] [PubMed] [Google Scholar]
  35. Youngs D. A., Smith K. C. Genetic control of multiple pathways of post-replicational repair in uvrB strains of Escherichia coli K-12. J Bacteriol. 1976 Jan;125(1):102–110. doi: 10.1128/jb.125.1.102-110.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES