Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1986 Dec;168(3):1459–1462. doi: 10.1128/jb.168.3.1459-1462.1986

Biosynthesis of Rhizobium trifolii capsular polysaccharide: enzymatic transfer of pyruvate substitutions into lipid-bound saccharide intermediates.

A E Gardiol, F B Dazzo
PMCID: PMC213661  PMID: 3782043

Abstract

The activity of capsular polysaccharide pyruvyltransferase catalyzing the pyruvylation of acidic heteropolysaccharide was measured in Rhizobium trifolii 843 and 0403 rif. This enzyme activity was determined with EDTA-treated cells, uridine diphosphate-sugar precursors, and phosphoenol [1-14C]pyruvate. Activity was measured by the incorporation of radioactivity into organic solvent-soluble glycoconjugates. Enzymatic pyruvylation of capsular polysaccharide occurred from phosphoenolpyruvate at the lipid-bound saccharide stage.

Full text

PDF
1462

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe M., Sherwood J. E., Hollingsworth R. I., Dazzo F. B. Stimulation of clover root hair infection by lectin-binding oligosaccharides from the capsular and extracellular polysaccharides of Rhizobium trifolii. J Bacteriol. 1984 Nov;160(2):517–520. doi: 10.1128/jb.160.2.517-520.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bossio J. C., de Iannino N. I., Dankert M. A. In vitro synthesis of a lipid-linked acetylated and pyruvilated oligosaccharide in Rhizobium trifolii. Biochem Biophys Res Commun. 1986 Jan 14;134(1):205–211. doi: 10.1016/0006-291x(86)90548-6. [DOI] [PubMed] [Google Scholar]
  3. Couso R. O., Ielpi L., Garcia R. C., Dankert M. A. Biosynthesis of polysaccharides in Acetobacter xylinum. Sequential synthesis of a heptasaccharide diphosphate prenol. Eur J Biochem. 1982 Apr;123(3):617–627. doi: 10.1111/j.1432-1033.1982.tb06577.x. [DOI] [PubMed] [Google Scholar]
  4. DEDONDER R. A., HASSID W. Z. THE ENZYMATIC SYNTHESIS OF A (BETA-I,2-)-LINKED GLUCAN BY AN EXTRACT OF RHIZOBIUM JAPONICUM. Biochim Biophys Acta. 1964 Aug 19;90:239–248. doi: 10.1016/0304-4165(64)90187-4. [DOI] [PubMed] [Google Scholar]
  5. Ielpi L., Couso R. O., Dankert M. A. Pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid. Biochem Biophys Res Commun. 1981 Oct 30;102(4):1400–1408. doi: 10.1016/s0006-291x(81)80167-2. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Lennarz W. J., Scher M. G. Metabolism and function of polyisoprenol sugar intermediates in membrane-associated reactions. Biochim Biophys Acta. 1972 Aug 4;265(3):417–441. doi: 10.1016/0304-4157(72)90015-9. [DOI] [PubMed] [Google Scholar]
  8. Sherwood J. E., Vasse J. M., Dazzo F. B., Truchet G. L. Development and trifoliin A-binding ability of the capsule of Rhizobium trifolii. J Bacteriol. 1984 Jul;159(1):145–152. doi: 10.1128/jb.159.1.145-152.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sutherland I. W. Biosynthesis and composition of gram-negative bacterial extracellular and wall polysaccharides. Annu Rev Microbiol. 1985;39:243–270. doi: 10.1146/annurev.mi.39.100185.001331. [DOI] [PubMed] [Google Scholar]
  10. Sutherland I. W., Norval M. The synthesis of exopolysaccharide by Klebsiella aerogenes membrane preparations and the involvement of lipid intermediates. Biochem J. 1970 Dec;120(3):567–576. doi: 10.1042/bj1200567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
  12. Tolmasky M. E., Staneloni R. J., Leloir L. F. Lipid-bound saccharides in Rhizobium meliloti. J Biol Chem. 1982 Jun 25;257(12):6751–6757. [PubMed] [Google Scholar]
  13. Tolmasky M. E., Staneloni R. J., Ugalde R. A., Leloir L. F. Lipid-bound sugars in Rhizobium meliloti. Arch Biochem Biophys. 1980 Aug;203(1):358–364. doi: 10.1016/0003-9861(80)90187-3. [DOI] [PubMed] [Google Scholar]
  14. Troy F. A., Frerman F. E., Heath E. C. The biosynthesis of capsular polysaccharide in Aerobacter aerogenes. J Biol Chem. 1971 Jan 10;246(1):118–133. [PubMed] [Google Scholar]
  15. Ugalde R. A., Handelsman J., Brill W. J. Role of galactosyltransferase activity in phage sensitivity and nodulation competitiveness of Rhizobium meliloti. J Bacteriol. 1986 Apr;166(1):148–154. doi: 10.1128/jb.166.1.148-154.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES