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. 1987 Sep;169(9):4036–4040. doi: 10.1128/jb.169.9.4036-4040.1987

Involvement of FtsZ protein in shift-up-induced division delay in Escherichia coli.

F Kepes, R D'Ari
PMCID: PMC213705  PMID: 3040674

Abstract

A nutritional shift-up from glucose minimal medium to LB broth was previously shown to cause a division delay of about 20 min in synchronized cultures of Escherichia coli, and a similar delay was observed after a nutritional pulse (a shift-up followed rapidly by a return to glucose minimal medium). Using synchronized cultures, we show here that the pulse-induced division delay does not require protein synthesis during the period in LB broth, suggesting that a nonprotein signal is generated by the shift-up and transmitted to the cell division machinery. The cell division protein FtsZ, target of the SOS-associated division inhibitor SfiA (or SulA), seems to be involved in the postshift division delay. Mutants in which the FtsZ-SfiA interaction is reduced, either sfiA (loss of SfiA) or ftsZ(SfiB) (modification of FtsZ), have a 50- to 60-min division delay after a shift-up. Furthermore, after a nutritional pulse, the ftsZ(SfiB) mutant had only a 10- to 16-min delay. These results suggest that the FtsZ protein is the target element of the cell division machinery to which the shift-up signal is transmitted.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burton P., Holland I. B. Two pathways of division inhibition in UV-irradiated E. coli. Mol Gen Genet. 1983;190(2):309–314. doi: 10.1007/BF00330656. [DOI] [PubMed] [Google Scholar]
  2. D'Ari R., Huisman O. Novel mechanism of cell division inhibition associated with the SOS response in Escherichia coli. J Bacteriol. 1983 Oct;156(1):243–250. doi: 10.1128/jb.156.1.243-250.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Helmstetter C., Cooper S., Pierucci O., Revelas E. On the bacterial life sequence. Cold Spring Harb Symp Quant Biol. 1968;33:809–822. doi: 10.1101/sqb.1968.033.01.093. [DOI] [PubMed] [Google Scholar]
  4. Holland I. B., Jones C. The role of the FtsZ protein (SfiB) in UV-induced division inhibition and in the normal Escherichia coli cell division cycle. Ann Inst Pasteur Microbiol. 1985 Jan-Feb;136A(1):165–171. doi: 10.1016/s0769-2609(85)80037-5. [DOI] [PubMed] [Google Scholar]
  5. Huisman O., D'Ari R. An inducible DNA replication-cell division coupling mechanism in E. coli. Nature. 1981 Apr 30;290(5809):797–799. doi: 10.1038/290797a0. [DOI] [PubMed] [Google Scholar]
  6. Huisman O., D'Ari R., George J. Inducible sfi dependent division inhibition in Escherichia coli. Mol Gen Genet. 1980;177(4):629–636. doi: 10.1007/BF00272673. [DOI] [PubMed] [Google Scholar]
  7. Huisman O., D'Ari R., Gottesman S. Cell-division control in Escherichia coli: specific induction of the SOS function SfiA protein is sufficient to block septation. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4490–4494. doi: 10.1073/pnas.81.14.4490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huisman O., Jacques M., D'ari R., Caro L. Role of the sfiA-dependent cell division regulation system in Escherichia coli. J Bacteriol. 1983 Feb;153(2):1072–1074. doi: 10.1128/jb.153.2.1072-1074.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jones C. A., Holland I. B. Inactivation of essential division genes, ftsA, ftsZ, suppresses mutations at sfiB, a locus mediating division inhibition during the SOS response in E. coli. EMBO J. 1984 May;3(5):1181–1186. doi: 10.1002/j.1460-2075.1984.tb01948.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jones C., Holland I. B. Role of the SulB (FtsZ) protein in division inhibition during the SOS response in Escherichia coli: FtsZ stabilizes the inhibitor SulA in maxicells. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6045–6049. doi: 10.1073/pnas.82.18.6045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KJELDGAARD N. O., MAALOE O., SCHAECHTER M. The transition between different physiological states during balanced growth of Salmonella typhimurium. J Gen Microbiol. 1958 Dec;19(3):607–616. doi: 10.1099/00221287-19-3-607. [DOI] [PubMed] [Google Scholar]
  12. Kepes F., Kepes A. Synchronisation automatique de la croissance de Escherichia coli. Ann Microbiol (Paris) 1980 Jan-Feb;131(1):3–16. [PubMed] [Google Scholar]
  13. Kogoma T., Lark K. G. Characterization of the replication of Escherichia coli DNA in the absence of protein synthesis: stable DNA replication. J Mol Biol. 1975 May 15;94(2):243–256. doi: 10.1016/0022-2836(75)90081-9. [DOI] [PubMed] [Google Scholar]
  14. Loeb A., McGrath B. E., Navre J. M., Pierucci O. Cell division during nutritional upshifts of Escherichia coli. J Bacteriol. 1978 Nov;136(2):631–637. doi: 10.1128/jb.136.2.631-637.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lutkenhaus J. F. Coupling of DNA replication and cell division: sulB is an allele of ftsZ. J Bacteriol. 1983 Jun;154(3):1339–1346. doi: 10.1128/jb.154.3.1339-1346.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Maguin E., Brody H., Hill C. W., D'Ari R. SOS-associated division inhibition gene sfiC is part of excisable element e14 in Escherichia coli. J Bacteriol. 1986 Oct;168(1):464–466. doi: 10.1128/jb.168.1.464-466.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mizusawa S., Gottesman S. Protein degradation in Escherichia coli: the lon gene controls the stability of sulA protein. Proc Natl Acad Sci U S A. 1983 Jan;80(2):358–362. doi: 10.1073/pnas.80.2.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nierlich D. P. Regulation of ribonucleic acid synthesis in growing bacterial cells. I. Control over the total rate of RNA synthesis. J Mol Biol. 1972 Dec 30;72(3):751–764. doi: 10.1016/0022-2836(72)90189-1. [DOI] [PubMed] [Google Scholar]
  19. Ryals J., Little R., Bremer H. Control of rRNA and tRNA syntheses in Escherichia coli by guanosine tetraphosphate. J Bacteriol. 1982 Sep;151(3):1261–1268. doi: 10.1128/jb.151.3.1261-1268.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sloan J. B., Urban J. E. Growth response of Escherichia coli to nutritional shift-up: immediate division stimulation in slow-growing cells. J Bacteriol. 1976 Oct;128(1):302–308. doi: 10.1128/jb.128.1.302-308.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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