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. 1973 Aug 1;138(2):473–478. doi: 10.1084/jem.138.2.473

HAPTEN-SPECIFIC STIMULATION OF SECONDARY B CELLS INDEPENDENT OF T CELLS

Norman R Klinman 1, Robert A Doughty 1
PMCID: PMC2139408  PMID: 4124212

Abstract

Treatment of spleen cell suspensions from immunized mice with anti-theta serum and complement before transfer to nonimmune irradiated recipients reduced the degree of in vitro stimulation by hapten-homologous carrier complexes by 90%, but did not decrease at all the number of isolated precursor cells stimulated by hapten on heterologous carriers. Thus, secondary B cells can be stimulated by low concentrations of multiply substituted hapten-carrier complexes in the apparent complete absence of specific T cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Askonas B. A., Williamson A. R. Factors affecting the propagation of a B cell clone forming antibody to the 2,4-dinitrophenyl group. Eur J Immunol. 1972 Dec;2(6):487–493. doi: 10.1002/eji.1830020603. [DOI] [PubMed] [Google Scholar]
  2. Bush M. E., Alkan S. S., Nitecki D. E., Goodman J. W. Antigen recognition and the immune response. "Self-help" with symmetrical bifunctional antigen molecules. J Exp Med. 1972 Dec 1;136(6):1478–1483. doi: 10.1084/jem.136.6.1478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Feldmann M., Easten A. The relationship between antigenic structure and the requirement for thymus-derived cells in the immune response. J Exp Med. 1971 Jul 1;134(1):103–119. doi: 10.1084/jem.134.1.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Howard J. G., Christie G. H., Courtenay B. M., Leuchars E., Davies A. J. Studies on immunological paralysis. VI. Thymic-independence of tolerance and immunity to type 3 pneumococcal polysaccharide. Cell Immunol. 1971 Dec;2(6):614–626. doi: 10.1016/0008-8749(71)90009-8. [DOI] [PubMed] [Google Scholar]
  5. Klinman N. R., Aschinazi G. The stimulation of splenic foci in vitro. J Immunol. 1971 May;106(5):1338–1344. [PubMed] [Google Scholar]
  6. Klinman N. R. The mechanism of antigenic stimulation of primary and secondary clonal precursor cells. J Exp Med. 1972 Aug 1;136(2):241–260. doi: 10.1084/jem.136.2.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Klinman N. R. The secondary immune response to a hapten in vitro. Antigen concentration and the carrier effect. J Exp Med. 1971 May 1;133(5):963–972. doi: 10.1084/jem.133.5.963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LEVINE B. B. STUDIES ON DELAYED HYPERSENSITIVITY. I. INFERENCES ON THE COMPARATIVE BINDING AFFINITIES OF ANTIBODIES MEDIATING DELAYED AND IMMEDIATE HYPERSENSITIVITY REACTIONS IN THE GUINEA PIG. J Exp Med. 1965 Jun 1;121:873–888. doi: 10.1084/jem.121.6.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miller J. F., Basten A., Sprent J., Cheers C. Interaction between lymphocytes in immune responses. Cell Immunol. 1971 Oct;2(5):469–495. doi: 10.1016/0008-8749(71)90057-8. [DOI] [PubMed] [Google Scholar]
  10. Mitchison N. A. The carrier effect in the secondary response to hapten-protein conjugates. I. Measurement of the effect with transferred cells and objections to the local environment hypothesis. Eur J Immunol. 1971 Jan;1(1):10–17. doi: 10.1002/eji.1830010103. [DOI] [PubMed] [Google Scholar]
  11. Mitchison N. A. The carrier effect in the secondary response to hapten-protein conjugates. II. Cellular cooperation. Eur J Immunol. 1971 Jan;1(1):18–27. doi: 10.1002/eji.1830010104. [DOI] [PubMed] [Google Scholar]
  12. Moorhead J. W., Walters C. S., Claman H. N. Immunologic reactions to haptens on autologous carriers. I. Participation of both thymus-derived and bone marrow-derived cells in the secondary in vitro response. J Exp Med. 1973 Feb 1;137(2):411–423. doi: 10.1084/jem.137.2.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. OVARY Z., BENACERRAF B. IMMUNOLOGICAL SPECIFICITY OF THE SECONDARY RESPONSE WITH DINITROPHENYLATED PROTEINS. Proc Soc Exp Biol Med. 1963 Oct;114:72–76. doi: 10.3181/00379727-114-28589. [DOI] [PubMed] [Google Scholar]
  14. Pinchuck P., Maurer P. H. Antigenicity of polypeptides (poly-alpha-amino acids). XXVI. Studies of the ability of homo- and copolymers to act as hapten carriers in mice. J Immunol. 1968 Feb;100(2):384–394. [PubMed] [Google Scholar]
  15. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rittenberg M. B., Campbell D. H. Heterologous carriers in the anamnestic antihapten response. J Exp Med. 1968 Apr 1;127(4):717–730. doi: 10.1084/jem.127.4.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WIGZELL H. QUANTITATIVE TITRATIONS OF MOUSE H-2 ANTIBODIES USING CR-51-LABELLED TARGET CELLS. Transplantation. 1965 May;3:423–431. doi: 10.1097/00007890-196505000-00011. [DOI] [PubMed] [Google Scholar]

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