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. 1987 Dec;169(12):5795–5800. doi: 10.1128/jb.169.12.5795-5800.1987

Isolation and characterization of temperature-sensitive pantothenate kinase (coaA) mutants of Escherichia coli.

D S Vallari 1, C O Rock 1
PMCID: PMC214146  PMID: 2824448

Abstract

Escherichia coli mutants conditionally defective in the conversion of pantothenate to coenzyme A were isolated and characterized. The gene was designated coaA and localized between argEH and rpoB near min 90 of the chromosome. The coaA15(Ts) mutation caused a temperature-sensitive growth phenotype and temperature-dependent inactivation of pantothenate kinase activity assayed both in vivo and in vitro. At 30 degrees C, coaA15(Ts) extracts contained less than 20% of the wild-type pantothenate kinase activity; the kinase had near normal kinetic constants for the substrates ATP and pantothenate and was inhibited by coenzyme A to the same degree as the wild-type enzyme. These data define the coaA gene as the structural gene for pantothenate kinase.

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Selected References

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