Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1987 Dec;169(12):5831–5834. doi: 10.1128/jb.169.12.5831-5834.1987

Nucleotide sequences of the genes encoding type 1 fimbrial subunits of Klebsiella pneumoniae and Salmonella typhimurium.

B K Purcell 1, J Pruckler 1, S Clegg 1
PMCID: PMC214169  PMID: 2890624

Abstract

The nucleotide sequences of the genes encoding the subunits of Klebsiella pneumoniae and Salmonella typhimurium type 1 fimbriae were determined. Comparison of the predicted amino acid sequences of the two subunits revealed domains in which the sequences were highly conserved. Both gene products possessed signal peptides, a fact consistent with the transport of the fimbrial subunit across the membrane, but these regions showed no amino acid homology between the two proteins. The predicted N-terminal amino acid sequences of the processed fimbrial subunits were in good agreement with those obtained by purification of the fimbrial subunits.

Full text

PDF
5832

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham J. M., Freitag C. S., Clements J. R., Eisenstein B. I. An invertible element of DNA controls phase variation of type 1 fimbriae of Escherichia coli. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5724–5727. doi: 10.1073/pnas.82.17.5724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brenner D. J. Characterization and clinical identification of Enterobacteriaceae by DNA hybridization. Prog Clin Pathol. 1978;7:71–117. [PubMed] [Google Scholar]
  3. Chou P. Y., Fasman G. D. Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol Relat Areas Mol Biol. 1978;47:45–148. doi: 10.1002/9780470122921.ch2. [DOI] [PubMed] [Google Scholar]
  4. Clegg S., Hull S., Hull R., Pruckler J. Construction and comparison of recombinant plasmids encoding type 1 fimbriae of members of the family Enterobacteriaceae. Infect Immun. 1985 May;48(2):275–279. doi: 10.1128/iai.48.2.275-279.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Duguid J. P., Campbell I. Antigens of the type-1 fimbriae of salmonellae and other enterobacteria. J Med Microbiol. 1969 Nov 4;2(4):535–553. doi: 10.1099/00222615-2-4-535. [DOI] [PubMed] [Google Scholar]
  6. Fader R. C., Duffy L. K., Davis C. P., Kurosky A. Purification and chemical characterization of type 1 pili isolated from Klebsiella pneumoniae. J Biol Chem. 1982 Mar 25;257(6):3301–3305. [PubMed] [Google Scholar]
  7. GILLIES R. R., DUGUID J. P. The fimbrial antigens of Shigella flexneri. J Hyg (Lond) 1958 Sep;56(3):303–318. doi: 10.1017/s0022172400037803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Klemm P. The fimA gene encoding the type-1 fimbrial subunit of Escherichia coli. Nucleotide sequence and primary structure of the protein. Eur J Biochem. 1984 Sep 3;143(2):395–399. doi: 10.1111/j.1432-1033.1984.tb08386.x. [DOI] [PubMed] [Google Scholar]
  9. Maurer L., Orndorff P. E. Identification and characterization of genes determining receptor binding and pilus length of Escherichia coli type 1 pili. J Bacteriol. 1987 Feb;169(2):640–645. doi: 10.1128/jb.169.2.640-645.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Michaelis S., Beckwith J. Mechanism of incorporation of cell envelope proteins in Escherichia coli. Annu Rev Microbiol. 1982;36:435–465. doi: 10.1146/annurev.mi.36.100182.002251. [DOI] [PubMed] [Google Scholar]
  11. Minion F. C., Abraham S. N., Beachey E. H., Goguen J. D. The genetic determinant of adhesive function in type 1 fimbriae of Escherichia coli is distinct from the gene encoding the fimbrial subunit. J Bacteriol. 1986 Mar;165(3):1033–1036. doi: 10.1128/jb.165.3.1033-1036.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nowotarska M., Mulczyk M. Serologic relationship of fimbriae among Enterobacteriaceae. Arch Immunol Ther Exp (Warsz) 1977;25(1):7–16. [PubMed] [Google Scholar]
  13. Orndorff P. E., Falkow S. Nucleotide sequence of pilA, the gene encoding the structural component of type 1 pili in Escherichia coli. J Bacteriol. 1985 Apr;162(1):454–457. doi: 10.1128/jb.162.1.454-457.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Purcell B. K., Clegg S. Construction and expression of recombinant plasmids encoding type 1 fimbriae of a urinary Klebsiella pneumoniae isolate. Infect Immun. 1983 Mar;39(3):1122–1127. doi: 10.1128/iai.39.3.1122-1127.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Spears P. A., Schauer D., Orndorff P. E. Metastable regulation of type 1 piliation in Escherichia coli and isolation and characterization of a phenotypically stable mutant. J Bacteriol. 1986 Oct;168(1):179–185. doi: 10.1128/jb.168.1.179-185.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES