Abstract
Membrane vesicles of Clostridium thermoautotrophicum prepared by osmotic lysis after lysozyme treatment contained carbon monoxide dehydrogenase and methylenetetrahydrofolate dehydrogenase with specific activities three- to fourfold higher than the specific activity of the cytoplasm. The membrane-associated carbon monoxide dehydrogenase mediated the reduction with CO or the oxidation with CO2 of b-type cytochromes and other electron carriers in the membrane.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Andreesen J. R., Schaupp A., Neurauter C., Brown A., Ljungdahl L. G. Fermentation of glucose, fructose, and xylose by Clostridium thermoaceticum: effect of metals on growth yield, enzymes, and the synthesis of acetate from CO 2 . J Bacteriol. 1973 May;114(2):743–751. doi: 10.1128/jb.114.2.743-751.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clark J. E., Ljungdahl L. G. Purification and properties of 5,10-methylenetetrahydrofolate reductase, an iron-sulfur flavoprotein from Clostridium formicoaceticum. J Biol Chem. 1984 Sep 10;259(17):10845–10849. [PubMed] [Google Scholar]
- Clark J. E., Ragsdale S. W., Ljungdahl L. G., Wiegel J. Levels of enzymes involved in the synthesis of acetate from CO2 in Clostridium thermoautotrophicum. J Bacteriol. 1982 Jul;151(1):507–509. doi: 10.1128/jb.151.1.507-509.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elliott J. I., Brewer J. M. The inactivation of yeast enolase by 2,3-butanedione. Arch Biochem Biophys. 1978 Sep;190(1):351–357. doi: 10.1016/0003-9861(78)90285-0. [DOI] [PubMed] [Google Scholar]
- Elliott J. I., Ljungdahl L. G. Isolation and characterization of an Fe,-S8 ferredoxin (ferredoxin II) from Clostridium thermoaceticum. J Bacteriol. 1982 Jul;151(1):328–333. doi: 10.1128/jb.151.1.328-333.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gottwald M., Andreesen J. R., LeGall J., Ljungdahl L. G. Presence of cytochrome and menaquinone in Clostridium formicoaceticum and Clostridium thermoaceticum. J Bacteriol. 1975 Apr;122(1):325–328. doi: 10.1128/jb.122.1.325-328.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ivey D. M., Ljungdahl L. G. Purification and characterization of the F1-ATPase from Clostridium thermoaceticum. J Bacteriol. 1986 Jan;165(1):252–257. doi: 10.1128/jb.165.1.252-257.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ljungdahl L. G., Andreesen J. R. Formate dehydrogenase, a selenium--tungsten enzyme from Clostridium thermoaceticum. Methods Enzymol. 1978;53:360–372. doi: 10.1016/s0076-6879(78)53042-5. [DOI] [PubMed] [Google Scholar]
- Ljungdahl L. G. The autotrophic pathway of acetate synthesis in acetogenic bacteria. Annu Rev Microbiol. 1986;40:415–450. doi: 10.1146/annurev.mi.40.100186.002215. [DOI] [PubMed] [Google Scholar]
- Mayer F., Ivey D. M., Ljungdahl L. G. Macromolecular organization of F1-ATPase isolated from Clostridium thermoaceticum as revealed by electron microscopy. J Bacteriol. 1986 Jun;166(3):1128–1130. doi: 10.1128/jb.166.3.1128-1130.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Otto R., Lageveen R. G., Veldkamp H., Konings W. N. Lactate efflux-induced electrical potential in membrane vesicles of Streptococcus cremoris. J Bacteriol. 1982 Feb;149(2):733–738. doi: 10.1128/jb.149.2.733-738.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ragsdale S. W., Clark J. E., Ljungdahl L. G., Lundie L. L., Drake H. L. Properties of purified carbon monoxide dehydrogenase from Clostridium thermoaceticum, a nickel, iron-sulfur protein. J Biol Chem. 1983 Feb 25;258(4):2364–2369. [PubMed] [Google Scholar]
- Ragsdale S. W., Ljungdahl L. G. Hydrogenase from Acetobacterium woodii. Arch Microbiol. 1984 Nov;139(4):361–365. doi: 10.1007/BF00408380. [DOI] [PubMed] [Google Scholar]
- Thauer R. K., Jungermann K., Decker K. Energy conservation in chemotrophic anaerobic bacteria. Bacteriol Rev. 1977 Mar;41(1):100–180. doi: 10.1128/br.41.1.100-180.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wakim B. T., Uffen R. L. Membrane association of the carbon monoxide oxidation system in Rhodopseudomonas gelatinosa. J Bacteriol. 1983 Jan;153(1):571–573. doi: 10.1128/jb.153.1.571-573.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]