Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1985 Nov;164(2):659–664. doi: 10.1128/jb.164.2.659-664.1985

Gene map for the Cyanophora paradoxa cyanelle genome.

D H Lambert, D A Bryant, V L Stirewalt, J M Dubbs, S E Stevens Jr, R D Porter
PMCID: PMC214303  PMID: 3932327

Abstract

The genes for the following proteins were localized by hybridization analysis on the cyanelle genome of Cyanophora paradoxa: the alpha and beta subunits of phycocyanin (cpcA and cpcB); the alpha and beta subunits of allophycocyanin (apcA and apcB); the large and small subunits of ribulose-1,5-bisphosphate carboxylase (rbcL and rbcS); the two putative chlorophyll alpha-binding apoproteins of the photosystem I-P700 complex (psaA and psaB); four apoproteins believed to be components of the photosystem II core complex (psbA, psbB, psbC, and psbD); the two apoprotein subunits of cytochrome b-559 which is also found in the core complex of photosystem II (psbE and psbF); three subunits of the ATP synthase complex (atpA and atpBE); and the cytochrome f apoprotein (petA). Eighty-five percent of the genome was cloned as BamHI, BglII, or PstI fragments. These cloned fragments were used to construct a physical map of the cyanelle genome and to localize more precisely some of the genes listed above. The genes for phycocyanin and allophycocyanin were not clustered and were separated by about 25 kilobases. Although the rbcL gene was adjacent to the atpBE genes and the psbC and psbD genes were adjacent, the arrangement of other genes encoding various polypeptide subunits of protein complexes involved in photosynthetic functions was dissimilar to that observed for known chloroplast genomes. These results are consistent with the independent development of this cyanelle from a cyanobacterial endosymbiont.

Full text

PDF
662

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alt J., Westhoff P., Sears B. B., Nelson N., Hurt E., Hauska G., Herrmann R. G. Genes and transcripts for the polypeptides of the cytochrome b6/f complex from spinach thylakoid membranes. EMBO J. 1983;2(6):979–986. doi: 10.1002/j.1460-2075.1983.tb01531.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Belford H. S., Offner G. D., Troxler R. F. Phycobiliprotein synthesis in the unicellular rhodophyte, Cyanidium caldarium. Cell-free translation of the mRNAs for the alpha and beta subunit polypeptides of phycocyanin. J Biol Chem. 1983 Apr 10;258(7):4503–4510. [PubMed] [Google Scholar]
  3. Bryant D. A., de Lorimier R., Lambert D. H., Dubbs J. M., Stirewalt V. L., Stevens S. E., Jr, Porter R. D., Tam J., Jay E. Molecular cloning and nucleotide sequence of the alpha and beta subunits of allophycocyanin from the cyanelle genome of Cyanophora paradoxa. Proc Natl Acad Sci U S A. 1985 May;82(10):3242–3246. doi: 10.1073/pnas.82.10.3242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Buzby J. S., Porter R. D., Stevens S. E., Jr Plasmid transformation in Agmenellum quadruplicatum PR-6: construction of biphasic plasmids and characterization of their transformation properties. J Bacteriol. 1983 Jun;154(3):1446–1450. doi: 10.1128/jb.154.3.1446-1450.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dron M., Rahire M., Rochaix J. D. Sequence of the chloroplast DNA region of Chlamydomonas reinhardii containing the gene of the large subunit of ribulose bisphosphate carboxylase and parts of its flanking genes. J Mol Biol. 1982 Dec 25;162(4):775–793. doi: 10.1016/0022-2836(82)90547-2. [DOI] [PubMed] [Google Scholar]
  6. Egelhoff T., Grossman A. Cytoplasmic and chloroplast synthesis of phycobilisome polypeptides. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3339–3343. doi: 10.1073/pnas.80.11.3339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fish L. E., Kück U., Bogorad L. Two partially homologous adjacent light-inducible maize chloroplast genes encoding polypeptides of the P700 chlorophyll a-protein complex of photosystem I. J Biol Chem. 1985 Feb 10;260(3):1413–1421. [PubMed] [Google Scholar]
  8. Giddings T. H., Wasmann C., Staehelin L. A. Structure of the Thylakoids and Envelope Membranes of the Cyanelles of Cyanophora paradoxa. Plant Physiol. 1983 Feb;71(2):409–419. doi: 10.1104/pp.71.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Godson G. N., Vapnek D. A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA. Biochim Biophys Acta. 1973 Apr 11;299(4):516–520. doi: 10.1016/0005-2787(73)90223-2. [DOI] [PubMed] [Google Scholar]
  10. Gray M. W., Doolittle W. F. Has the endosymbiont hypothesis been proven? Microbiol Rev. 1982 Mar;46(1):1–42. doi: 10.1128/mr.46.1.1-42.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hanahan D., Meselson M. Plasmid screening at high colony density. Methods Enzymol. 1983;100:333–342. doi: 10.1016/0076-6879(83)00066-x. [DOI] [PubMed] [Google Scholar]
  12. Lemaux P. G., Grossman A. Isolation and characterization of a gene for a major light-harvesting polypeptide from Cyanophora paradoxa. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4100–4104. doi: 10.1073/pnas.81.13.4100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Morris J., Herrmann R. G. Nucleotide sequence of the gene for the P680 chlorophyll alpha apoprotein of the photosystem II reaction center from spinach. Nucleic Acids Res. 1984 Mar 26;12(6):2837–2850. doi: 10.1093/nar/12.6.2837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mubumbila M., Gordon K. H., Crouse E. J., Burkard G., Weil J. H. Construction of the physical map of the chloroplast DNA of Phaseolus vulgaris and localization of ribosomal and transfer RNA genes. Gene. 1983 Mar;21(3):257–266. doi: 10.1016/0378-1119(83)90009-4. [DOI] [PubMed] [Google Scholar]
  16. Nierzwicki-Bauer S. A., Curtis S. E., Haselkorn R. Cotranscription of genes encoding the small and large subunits of ribulose-1,5-bisphosphate carboxylase in the cyanobacterium Anabaena 7120. Proc Natl Acad Sci U S A. 1984 Oct;81(19):5961–5965. doi: 10.1073/pnas.81.19.5961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shinozaki K., Sugiura M. The gene for the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase is located close to the gene for the large subunit in the cyanobacterium Anacystis nidulans 6301. Nucleic Acids Res. 1983 Oct 25;11(20):6957–6964. doi: 10.1093/nar/11.20.6957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Smeekens S., van Binsbergen J., Weisbeek P. The plant ferredoxin precursor: nucleotide sequence of a full length cDNA clone. Nucleic Acids Res. 1985 May 10;13(9):3179–3194. doi: 10.1093/nar/13.9.3179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  20. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  21. Westhoff P., Alt J., Herrmann R. G. Localization of the genes for the two chlorophyll a-conjugated polypeptides (mol. wt. 51 and 44 kd) of the photosystem II reaction center on the spinach plastid chromosome. EMBO J. 1983;2(12):2229–2237. doi: 10.1002/j.1460-2075.1983.tb01728.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Willey D. L., Auffret A. D., Gray J. C. Structure and topology of cytochrome f in pea chloroplast membranes. Cell. 1984 Feb;36(2):555–562. doi: 10.1016/0092-8674(84)90248-4. [DOI] [PubMed] [Google Scholar]
  23. de Lorimier R., Bryant D. A., Porter R. D., Liu W. Y., Jay E., Stevens S. E., Jr Genes for the alpha and beta subunits of phycocyanin. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7946–7950. doi: 10.1073/pnas.81.24.7946. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES