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. 1985 Nov;164(2):749–756. doi: 10.1128/jb.164.2.749-756.1985

Mathematical model for determining the effects of intracytoplasmic inclusions on volume and density of microorganisms.

J Mas, C Pedrós-Alió, R Guerrero
PMCID: PMC214315  PMID: 3902798

Abstract

Procaryotic microorganisms accumulate several polymers in the form of intracellular inclusions as a strategy to increase survival in a changing environment. Such inclusions avoid osmotic pressure increases by tightly packaging certain macromolecules into the inclusion. In the present paper, a model describing changes in volume and density of the microbial cell as a function of the weight of the macromolecule forming the inclusion is derived from simple theoretical principles. The model is then tested by linear regression with experimental data from glycogen accumulation in Escherichia coli, poly-beta-hydroxybutyrate accumulation in Alcaligenes eutrophus, and sulfur accumulation in Chromatium spp. The model predicts a certain degree of hydration of the polymer in the inclusion and explains both the linear relationship between volume of the cell and weight of the polymer and the hyperbolic relationship between density of the cell and weight of the polymer. Other implications of the model are also discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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