Abstract
The phs mutation, which causes a pleiotropic growth defect, has been mapped and shown to be an allele of rpoA, the gene for the alpha subunit of RNA polymerase. The mutation is shown to cause a transcription defect in the arabinose operon, araBAD.
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Selected References
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- Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Booth I. R., Epstein W., Giffard P. M., Rowland G. C. Roles of the trkB and trkC gene products of Escherichia coli in K+ transport. Biochimie. 1985 Jan;67(1):83–89. doi: 10.1016/s0300-9084(85)80233-9. [DOI] [PubMed] [Google Scholar]
- Casadaban M. J., Cohen S. N. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530–4533. doi: 10.1073/pnas.76.9.4530. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cerretti D. P., Dean D., Davis G. R., Bedwell D. M., Nomura M. The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene. Nucleic Acids Res. 1983 May 11;11(9):2599–2616. doi: 10.1093/nar/11.9.2599. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ENGLESBERG E., ANDERSON R. L., WEINBERG R., LEE N., HOFFEE P., HUTTENHAUER G., BOYER H. L-Arabinose-sensitive, L-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli. J Bacteriol. 1962 Jul;84:137–146. doi: 10.1128/jb.84.1.137-146.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Englesberg E., Irr J., Power J., Lee N. Positive control of enzyme synthesis by gene C in the L-arabinose system. J Bacteriol. 1965 Oct;90(4):946–957. doi: 10.1128/jb.90.4.946-957.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Essenberg R. C., Kornberg H. L. Location of the gene specifying hexose phosphate transport (uhp) on the chromosome of Escherichia coli. J Gen Microbiol. 1977 Mar;99(1):157–169. doi: 10.1099/00221287-99-1-157. [DOI] [PubMed] [Google Scholar]
- Fujiki H., Palm P., Zillig W., Calendar R., Sunshine M. Identification of a mutation within the structural gene for the a subunit of DNA-dependent RNA polymerase of E. coli. Mol Gen Genet. 1976 Apr 23;145(1):19–22. doi: 10.1007/BF00331552. [DOI] [PubMed] [Google Scholar]
- Giffard P. M., Rowland G. C., Kroll R. G., Stewart L. M., Bakker E. P., Booth I. R. Phenotypic properties of a unique rpoA mutation (phs) of Escherichia coli. J Bacteriol. 1985 Nov;164(2):904–910. doi: 10.1128/jb.164.2.904-910.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jaskunas S. R., Fallon A. M., Nomura M. Identification and organization of ribosomal protein genes of Escherichia coli carried by lambdafus2 transducing phage. J Biol Chem. 1977 Oct 25;252(20):7323–7336. [PubMed] [Google Scholar]
- Meek D. W., Hayward R. S. Nucleotide sequence of the rpoA-rplQ DNA of Escherichia coli: a second regulatory binding site for protein S4? Nucleic Acids Res. 1984 Jul 25;12(14):5813–5821. doi: 10.1093/nar/12.14.5813. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ovchinnikov Y. A., Lipkin V. M., Modyanov N. N., Chertov O. Y., Smirnov Y. V. Primary structure of alpha-subunit of DNA-dependent RNA polymerase from Escherichia coli. FEBS Lett. 1977 Apr 1;76(1):108–111. doi: 10.1016/0014-5793(77)80131-2. [DOI] [PubMed] [Google Scholar]
- Post L. E., Nomura M. Nucleotide sequence of the intercistronic region preceding the gene for RNA polymerase subunit alpha in Escherichia coli. J Biol Chem. 1979 Nov 10;254(21):10604–10606. [PubMed] [Google Scholar]
- Qureshi M. A., Smith D. A., Kingsman A. J. Mutants of Salmonella typhimurium responding to cysteine or methionine: their nature and possible role in the regulation of cysteine biosynthesis. J Gen Microbiol. 1975 Aug;89(2):353–370. doi: 10.1099/00221287-89-2-353. [DOI] [PubMed] [Google Scholar]
- Rowland G. C., Giffard P. M., Booth I. R. Genetic studies of the phs locus of Escherichia coli, a mutation causing pleiotropic lesions in metabolism and pH homeostasis. FEBS Lett. 1984 Aug 6;173(2):295–300. doi: 10.1016/0014-5793(84)80794-2. [DOI] [PubMed] [Google Scholar]
- Sunshine M. G., Sauer B. A bacterial mutation blocking P2 phage late gene expression. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2770–2774. doi: 10.1073/pnas.72.7.2770. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zilberstein D., Agmon V., Schuldiner S., Padan E. Escherichia coli intracellular pH, membrane potential, and cell growth. J Bacteriol. 1984 Apr;158(1):246–252. doi: 10.1128/jb.158.1.246-252.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zilberstein D., Agmon V., Schuldiner S., Padan E. The sodium/proton antiporter is part of the pH homeostasis mechanism in Escherichia coli. J Biol Chem. 1982 Apr 10;257(7):3687–3691. [PubMed] [Google Scholar]
- Zilberstein D., Ophir I. J., Padan E., Schuldiner S. Na+ gradient-coupled porters of EScherichia coli share a common subunit. J Biol Chem. 1982 Apr 10;257(7):3692–3696. [PubMed] [Google Scholar]
- Zillig W., Fujiki H., Blum W., Janeković D., Schweiger M., Rahmsdorf H., Ponta H., Hirsch-Kauffmann M. In vivo and in vitro phosphorylation of DNA-dependent RNA polymerase of Escherichia coli by bacteriophage-T7-induced protein kinase. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2506–2510. doi: 10.1073/pnas.72.7.2506. [DOI] [PMC free article] [PubMed] [Google Scholar]