Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1986 Mar;165(3):1019–1022. doi: 10.1128/jb.165.3.1019-1022.1986

Inhibition of citric acid accumulation by manganese ions in Aspergillus niger mutants with reduced citrate control of phosphofructokinase.

G Schreferl, C P Kubicek, M Röhr
PMCID: PMC214531  PMID: 2936730

Abstract

Mutant strains of Aspergillus niger with reduced citrate control of carbohydrate catabolism (cic mutants) grow faster than the parent strain on media containing 5% (wt/vol) citrate. The mutants tolerated a higher intracellular citrate concentration than the parent strain. One mutant (cic-7/3) contained phosphofructokinase activity significantly less sensitive towards citrate than the enzyme from the parent strain. When this mutant was grown under citrate-accumulating conditions, acidogenesis was far less sensitive to inhibition by Mn2+ than in the parent strain. Some of the cic mutants also showed altered citrate inhibition of NADP-specific isocitrate dehydrogenase.

Full text

PDF
1021

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  2. Habison A., Kubicek C. P., Röhr M. Partial purification and regulatory properties of phosphofructokinase from Aspergillus niger. Biochem J. 1983 Mar 1;209(3):669–676. doi: 10.1042/bj2090669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kisser M., Kubicek C. P., Röhr M. Influence of manganese on morphology and cell wall composition of Aspergillus niger during citric acid fermentation. Arch Microbiol. 1980 Nov;128(1):26–33. doi: 10.1007/BF00422301. [DOI] [PubMed] [Google Scholar]
  4. Kubicek C. P., Hampel W., Röhr M. Manganese deficiency leads to elevated amino acid pools in citric acid accumulating Aspergillus niger. Arch Microbiol. 1979 Oct;123(1):73–79. doi: 10.1007/BF00403504. [DOI] [PubMed] [Google Scholar]
  5. MAITRA P. K., ESTABROOK R. W. A FLUOROMETRIC METHOD FOR THE ENZYMIC DETERMINATION OF GLYCOLYTIC INTERMEDIATES. Anal Biochem. 1964 Apr;7:472–484. doi: 10.1016/0003-2697(64)90156-3. [DOI] [PubMed] [Google Scholar]
  6. Ma H., Kubicek C. P., Röhr M. Metabolic effects of manganese deficiency in Aspergillus niger: evidence for increased protein degradation. Arch Microbiol. 1985 Apr;141(3):266–268. doi: 10.1007/BF00408070. [DOI] [PubMed] [Google Scholar]
  7. Meixner-Monori B., Kubicek C. P., Habison A., Kubicek-Pranz E. M., Röhr M. Presence and regulation of the alpha-ketoglutarate dehydrogenase multienzyme complex in the filamentous fungus Aspergillus niger. J Bacteriol. 1985 Jan;161(1):265–271. doi: 10.1128/jb.161.1.265-271.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Montenecourt B. S., Eveleigh D. E. Semiquantitative Plate Assay for Determination of Cellulase Production by Trichoderma viride. Appl Environ Microbiol. 1977 Jan;33(1):178–183. doi: 10.1128/aem.33.1.178-183.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SLAYMAN C. W., TATUM E. L. POTASSIUM TRANSPORT IN NEUROSPORA. I. INTRACELLULAR SODIUM AND POTASSIUM CONCENTRATIONS, AND CATION REQUIREMENTS FOR GROWTH. Biochim Biophys Acta. 1964 Nov 29;88:578–592. [PubMed] [Google Scholar]
  10. Uhr M. L., Thompson V. W., Cleland W. W. The kinetics of pig heart triphosphopyridine nucleotide-isocitrate dehydrogenase. I. Initial velocity, substrate and product inhibition, and isotope exchange studies. J Biol Chem. 1974 May 10;249(9):2920–2927. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES