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. 1986 Apr;166(1):205–211. doi: 10.1128/jb.166.1.205-211.1986

Nucleotide pool in pho regulon mutants and alkaline phosphatase synthesis in Escherichia coli.

N N Rao, E Wang, J Yashphe, A Torriani
PMCID: PMC214577  PMID: 3514576

Abstract

The intracellular nucleotide pool of Escherichia coli W3110 reproducibly changes from conditions of growth in phosphate excess to phosphate starvation, with at least two nucleotides appearing under starvation conditions and two nucleotides appearing only under excess phosphate conditions. Strains bearing a deletion of the phoA gene show the same pattern, indicating that dephosphorylation by alkaline phosphatase is not responsible for the changes. Strains with mutations in the phoU gene, which result in constitutive expression of the pho regulon, show the nucleotide pattern of phosphate-starved cells even during phosphate excess growth. These changes in nucleotides are therefore due to phoU mutation but not to alkaline phosphatase constitutivity. In fact, a phoR (phoR68) mutant strain has the patterns of the wild type in spite of being constitutive for alkaline phosphatase. That these nucleotides might be specific signals for pho regulon expression was supported by the fact that the two nucleotides appearing under phosphate starvation induced the synthesis of alkaline phosphatase in repressed permeabilized wild-type cells under conditions of phosphate excess.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bochner B. R., Ames B. N. Complete analysis of cellular nucleotides by two-dimensional thin layer chromatography. J Biol Chem. 1982 Aug 25;257(16):9759–9769. [PubMed] [Google Scholar]
  3. GAREN A., ECHOLS H. Properties of two regulating genes for alkaline phosphatase. J Bacteriol. 1962 Feb;83:297–300. doi: 10.1128/jb.83.2.297-300.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Guan C. D., Wanner B., Inouye H. Analysis of regulation of phoB expression using a phoB-cat fusion. J Bacteriol. 1983 Nov;156(2):710–717. doi: 10.1128/jb.156.2.710-717.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Neidhardt F. C., Bloch P. L., Smith D. F. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. doi: 10.1128/jb.119.3.736-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pratt C. Kinetics and regulation of cell-free alkaline phosphatase synthesis. J Bacteriol. 1980 Sep;143(3):1265–1274. doi: 10.1128/jb.143.3.1265-1274.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Shinagawa H., Makino K., Nakata A. Regulation of the pho regulon in Escherichia coli K-12. Genetic and physiological regulation of the positive regulatory gene phoB. J Mol Biol. 1983 Aug 15;168(3):477–488. doi: 10.1016/s0022-2836(83)80297-6. [DOI] [PubMed] [Google Scholar]
  8. TORRIANI A. Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. Biochim Biophys Acta. 1960 Mar 11;38:460–469. doi: 10.1016/0006-3002(60)91281-6. [DOI] [PubMed] [Google Scholar]
  9. Tetu C., Dassa E., Boquet P. L. Unusual pattern of nucleoside polyphosphate hydrolysis by the acid phosphatase (optimum pH = 2.5) of Escherichia coli. Biochem Biophys Res Commun. 1979 Mar 15;87(1):314–322. doi: 10.1016/0006-291x(79)91681-4. [DOI] [PubMed] [Google Scholar]
  10. Tommassen J., de Geus P., Lugtenberg B., Hackett J., Reeves P. Regulation of the pho regulon of Escherichia coli K-12. Cloning of the regulatory genes phoB and phoR and identification of their gene products. J Mol Biol. 1982 May 15;157(2):265–274. doi: 10.1016/0022-2836(82)90233-9. [DOI] [PubMed] [Google Scholar]
  11. Torriani A. Alkaline phosphatase subunits and their dimerization in vivo. J Bacteriol. 1968 Oct;96(4):1200–1207. doi: 10.1128/jb.96.4.1200-1207.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tribhuwan R. C., Pradhan D. S. Induction of alkaline phosphatase in Escherichia coli. Effect of phenethyl alcohol. Biochim Biophys Acta. 1977 Sep 20;478(2):215–223. doi: 10.1016/0005-2787(77)90185-x. [DOI] [PubMed] [Google Scholar]
  13. Wanner B. L., Latterell P. Mutants affected in alkaline phosphatase, expression: evidence for multiple positive regulators of the phosphate regulon in Escherichia coli. Genetics. 1980 Oct;96(2):353–366. doi: 10.1093/genetics/96.2.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wilkins A. S. Physiological factors in the regulation of alkaline phosphatase synthesis in Escherichia coli. J Bacteriol. 1972 May;110(2):616–623. doi: 10.1128/jb.110.2.616-623.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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