Abstract
Polyadenylated [poly(A)+] RNA has been isolated from the halophilic archaebacterium Halobacterium halobium by binding, at 4 degrees C, to oligo(dT)-cellulose. H. halobium contains approximately 12 times more poly(A) per unit of RNA than does the methanogenic archaebacterium Methanococcus vannielii. The 3' poly(A) tracts in poly(A)+ RNA molecules are approximately twice as long (average length of 20 nucleotides) in H. halobium as in M. vannielii. In both archaebacterial species, poly(A)+ RNAs are unstable.
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- Brown J. W., Reeve J. N. Polyadenylated, noncapped RNA from the archaebacterium Methanococcus vannielii. J Bacteriol. 1985 Jun;162(3):909–917. doi: 10.1128/jb.162.3.909-917.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chang S. H., Majumdar A., Dunn R., Makabe O., RajBhandary U. L., Khorana H. G., Ohtsuka E., Tanaka T., Taniyama Y. O., Ikehara M. Bacteriorhodopsin: partial sequence of mRNA provides amino acid sequence in the precursor region. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3398–3402. doi: 10.1073/pnas.78.6.3398. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crouch D. H., Ownby J. D., Carr N. G. Polyadenylated RNA in two filamentous cyanobacteria. J Bacteriol. 1983 Nov;156(2):979–982. doi: 10.1128/jb.156.2.979-982.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hussain I., Tsukagoshi N., Udaka S. Characterization of polyadenylated RNA in a protein-producing bacterium, Bacillus brevis 47. J Bacteriol. 1982 Sep;151(3):1162–1170. doi: 10.1128/jb.151.3.1162-1170.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Majumdar P. K., McFadden B. A. Polyadenylated mRNA from the photosynthetic procaryote Rhodospirillum rubrum. J Bacteriol. 1984 Mar;157(3):795–801. doi: 10.1128/jb.157.3.795-801.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moritz A., Lankat-Buttgereit B., Gross H. J., Goebel W. Common structural features of the genes for two stable RNAs from Halobacterium halobium. Nucleic Acids Res. 1985 Jan 11;13(1):31–43. doi: 10.1093/nar/13.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oesterhelt D., Stoeckenius W. Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane. Methods Enzymol. 1974;31:667–678. doi: 10.1016/0076-6879(74)31072-5. [DOI] [PubMed] [Google Scholar]
- Ohta N., Sanders M., Newton A. Characterization of unstable poly (A)-RNA in Caulobacter crescentus. Biochim Biophys Acta. 1978 Jan 26;517(1):65–75. doi: 10.1016/0005-2787(78)90034-5. [DOI] [PubMed] [Google Scholar]
- Schultz G. A., Chaconas G., Moore R. L. Polyadenylic acid sequences in the RNA of Hyphomicrobium. J Bacteriol. 1978 Feb;133(2):569–575. doi: 10.1128/jb.133.2.569-575.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]