Abstract
A mutant strain of Bacillus subtilis carrying lesions in the structural genes for extracellular neutral (nprE) and serine (aprA) proteases was constructed by the gene conversion technique. This mutant had less than 4% of the extracellular protease activity of the wild type and sporulated normally, indicating that neither of these sporulation-associated proteases is essential for development.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Goldfarb D. S., Wong S. L., Kudo T., Doi R. H. A temporally regulated promoter from Bacillus subtilis is transcribed only by an RNA polymerase with a 37,000 dalton sigma factor. Mol Gen Genet. 1983;191(2):319–325. doi: 10.1007/BF00334833. [DOI] [PubMed] [Google Scholar]
- Gryczan T. J., Contente S., Dubnau D. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J Bacteriol. 1978 Apr;134(1):318–329. doi: 10.1128/jb.134.1.318-329.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoch J. A. Genetics of bacterial sporulation. Adv Genet. 1976;18:69–98. doi: 10.1016/s0065-2660(08)60437-x. [DOI] [PubMed] [Google Scholar]
- Leighton T. J., Dor R. H., Warren R. A., Kelln R. A. The relationship of serine protease activity to RNA polymerase modification and sporulation in Bacillus subtilis. J Mol Biol. 1973 May 5;76(1):103–122. doi: 10.1016/0022-2836(73)90083-1. [DOI] [PubMed] [Google Scholar]
- Michel J. F., Millet J. Physiological studies on early-blocked sporulation mutants of Bacillus subtilis. J Appl Bacteriol. 1970 Mar;33(1):220–227. doi: 10.1111/j.1365-2672.1970.tb05246.x. [DOI] [PubMed] [Google Scholar]
- Millet J. Characterization of proteinases excreted by Bacillus subtilis Marburg strain during sporulation. J Appl Bacteriol. 1970 Mar;33(1):207–219. doi: 10.1111/j.1365-2672.1970.tb05245.x. [DOI] [PubMed] [Google Scholar]
- Piggot P. J., Coote J. G. Genetic aspects of bacterial endospore formation. Bacteriol Rev. 1976 Dec;40(4):908–962. doi: 10.1128/br.40.4.908-962.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roitsch C. A., Hageman J. H. Bacillopeptidase F: two forms of a glycoprotein serine protease from Bacillus subtilis 168. J Bacteriol. 1983 Jul;155(1):145–152. doi: 10.1128/jb.155.1.145-152.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sadaie Y., Kada T. Formation of competent Bacillus subtilis cells. J Bacteriol. 1983 Feb;153(2):813–821. doi: 10.1128/jb.153.2.813-821.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shoer R., Rappaport H. P. Analysis of a Bacillus subtilis proteinase mutant. J Bacteriol. 1972 Feb;109(2):575–583. doi: 10.1128/jb.109.2.575-583.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Stahl M. L., Ferrari E. Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation. J Bacteriol. 1984 May;158(2):411–418. doi: 10.1128/jb.158.2.411-418.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Uehara H., Yamane K., Maruo B. Thermosensitive, extracellular neutral proteases in Bacillus subtilis: isolation, characterization, and genetics. J Bacteriol. 1979 Aug;139(2):583–590. doi: 10.1128/jb.139.2.583-590.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wong S. L., Price C. W., Goldfarb D. S., Doi R. H. The subtilisin E gene of Bacillus subtilis is transcribed from a sigma 37 promoter in vivo. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1184–1188. doi: 10.1073/pnas.81.4.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]