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. 1984 Oct;160(1):454–457. doi: 10.1128/jb.160.1.454-457.1984

Monoclonal antibodies to Rhizobium meliloti and surface mutants insensitive to them.

E Johansen, T M Finan, M L Gefter, E R Signer
PMCID: PMC214744  PMID: 6480561

Abstract

Monoclonal antibodies were produced to the surface of the symbiotic nitrogen-fixing bacterium Rhizobium meliloti. Bacterial lysis in the presence of complement or cycles of agglutination and growth were used to select mutants no longer recognized by the antibodies. The mutants were used to produce new antibodies with different specificities. Several mutants had altered sensitivity to one or more bacteriophages. R. meliloti strains from different sources had distinct patterns of sensitivity to monoclonal antibodies and phages, which together can be used for discriminative typing.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames P., Schluederberg S. A., Bergman K. Behavioral mutants of Rhizobium meliloti. J Bacteriol. 1980 Feb;141(2):722–727. doi: 10.1128/jb.141.2.722-727.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bayer E. A., Rosenberg E., Gutnick D. The isolation of cell surface mutants of Acinetobacter calcoaceticus RAG-1. J Gen Microbiol. 1981 Dec;127(2):295–300. doi: 10.1099/00221287-127-2-295. [DOI] [PubMed] [Google Scholar]
  3. Carrasco N., Tahara S. M., Patel L., Goldkorn T., Kaback H. R. Preparation, characterization, and properties of monoclonal antibodies against the lac carrier protein from Escherichia coli. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6894–6898. doi: 10.1073/pnas.79.22.6894. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coffino P., Baumal R., Laskov R., Scharff M. D. Cloning of mouse myeloma cells and detection of rare variants. J Cell Physiol. 1972 Jun;79(3):429–440. doi: 10.1002/jcp.1040790313. [DOI] [PubMed] [Google Scholar]
  5. Dazzo F. B., Hubbell D. H. Cross-reactive antigens and lectin as determinants of symbiotic specificity in the Rhizobium-clover association. Appl Microbiol. 1975 Dec;30(6):1017–1033. doi: 10.1128/am.30.6.1017-1033.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Emini E. A., Jameson B. A., Lewis A. J., Larsen G. R., Wimmer E. Poliovirus neutralization epitopes: analysis and localization with neutralizing monoclonal antibodies. J Virol. 1982 Sep;43(3):997–1005. doi: 10.1128/jvi.43.3.997-1005.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Finan T. M., Hartweig E., LeMieux K., Bergman K., Walker G. C., Signer E. R. General transduction in Rhizobium meliloti. J Bacteriol. 1984 Jul;159(1):120–124. doi: 10.1128/jb.159.1.120-124.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gabay J., Schwartz M. Monoclonal antibody as a probe for structure and function of an Escherichia coli outer membrane protein. J Biol Chem. 1982 Jun 25;257(12):6627–6630. [PubMed] [Google Scholar]
  9. Gerhard W., Webster R. G. Antigenic drift in influenza A viruses. I. Selection and characterization of antigenic variants of A/PR/8/34 (HON1) influenza virus with monoclonal antibodies. J Exp Med. 1978 Aug 1;148(2):383–392. doi: 10.1084/jem.148.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kearney J. F., Radbruch A., Liesegang B., Rajewsky K. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol. 1979 Oct;123(4):1548–1550. [PubMed] [Google Scholar]
  11. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  12. Marshak-Rothstein A., Fink P., Gridley T., Raulet D. H., Bevan M. J., Gefter M. L. Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus. J Immunol. 1979 Jun;122(6):2491–2497. [PubMed] [Google Scholar]
  13. Meade H. M., Signer E. R. Genetic mapping of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1977 May;74(5):2076–2078. doi: 10.1073/pnas.74.5.2076. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nowinski R. C., Tam M. R., Goldstein L. C., Stong L., Kuo C. C., Corey L., Stamm W. E., Handsfield H. H., Knapp J. S., Holmes K. K. Monoclonal antibodies for diagnosis of infectious diseases in humans. Science. 1983 Feb 11;219(4585):637–644. doi: 10.1126/science.6297006. [DOI] [PubMed] [Google Scholar]
  15. Paau A. S., Leps W. T., Brill W. J. Agglutinin from Alfalfa Necessary for Binding and Nodulation by Rhizobium meliloti. Science. 1981 Sep 25;213(4515):1513–1515. doi: 10.1126/science.213.4515.1513. [DOI] [PubMed] [Google Scholar]
  16. Raleigh E. A., Signer E. R. Positive selection of nodulation-deficient Rhizobium phaseoli. J Bacteriol. 1982 Jul;151(1):83–88. doi: 10.1128/jb.151.1.83-88.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Robertson S. M., Kettman J. R., Miller J. N., Norgard M. V. Murine monoclonal antibodies specific for virulent Treponema pallidum (Nichols). Infect Immun. 1982 Jun;36(3):1076–1085. doi: 10.1128/iai.36.3.1076-1085.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shulman M., Wilde C. D., Köhler G. A better cell line for making hybridomas secreting specific antibodies. Nature. 1978 Nov 16;276(5685):269–270. doi: 10.1038/276269a0. [DOI] [PubMed] [Google Scholar]
  19. Yelton D. E., Scharff M. D. Monoclonal antibodies: a powerful new tool in biology and medicine. Annu Rev Biochem. 1981;50:657–680. doi: 10.1146/annurev.bi.50.070181.003301. [DOI] [PubMed] [Google Scholar]
  20. Yewdell J. W., Webster R. G., Gerhard W. U. Antigenic variation in three distinct determinants of an influenza type A haemagglutinin molecule. Nature. 1979 May 17;279(5710):246–248. doi: 10.1038/279246a0. [DOI] [PubMed] [Google Scholar]

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