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. 1985 Jan;161(1):243–248. doi: 10.1128/jb.161.1.243-248.1985

Location of some proteins involved in peptidoglycan synthesis and cell division in the inner and outer membranes of Escherichia coli.

A Rodríguez-Tébar, J A Barbas, D Vázquez
PMCID: PMC214863  PMID: 3881388

Abstract

Inner and outer membranes of Escherichia coli were separated by isopycnic centrifugation in sucrose gradients and analyzed for the presence of penicillin-binding proteins. All penicillin-binding proteins--except penicillin-binding protein 3, which is found almost exclusively in the cytoplasmic membrane and is involved in septum formation--are also found in gradient fractions corresponding to the outer membrane. Our results support the hypothesis that approximately half of the total amount of penicillin-binding proteins may be sacculus-located proteins linked to the outer membrane, probably through peptidoglycan bridges.

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Selected References

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