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. 1985 Jan;161(1):272–276. doi: 10.1128/jb.161.1.272-276.1985

A mutation in Escherichia coli K-12 results in a requirement for thiamine and a decrease in L-serine deaminase activity.

E B Newman, B Miller, L D Colebrook, C Walker
PMCID: PMC214867  PMID: 3918001

Abstract

Mutants of Escherichia coli K-12 deficient in L-serine deaminase (L-SD) activity have been isolated. These strains required thiamine and grew normally when it was provided. The decrease in L-SD activity caused no obvious metabolic deficiency. A study of revertants and transductants showed that a single mutation was responsible for the thiamine requirement and for the decrease in L-SD activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker T. A., Howe M. M., Gross C. A. Mu dX, a derivative of Mu d1 (lac Apr) which makes stable lacZ fusions at high temperature. J Bacteriol. 1983 Nov;156(2):970–974. doi: 10.1128/jb.156.2.970-974.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown G. M., Williamson J. M. Biosynthesis of riboflavin, folic acid, thiamine, and pantothenic acid. Adv Enzymol Relat Areas Mol Biol. 1982;53:345–381. doi: 10.1002/9780470122983.ch9. [DOI] [PubMed] [Google Scholar]
  4. Casadaban M. J., Cohen S. N. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530–4533. doi: 10.1073/pnas.76.9.4530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dietrich J., Henning U. Regulation of pyruvate dehydrogenase complex synthesis in Escherichia coli K 12. Identification of the inducing metabolite. Eur J Biochem. 1970 Jun;14(2):258–269. doi: 10.1111/j.1432-1033.1970.tb00285.x. [DOI] [PubMed] [Google Scholar]
  6. Imamura N., Nakayama H. thiK and thiL loci of Escherichia coli. J Bacteriol. 1982 Aug;151(2):708–717. doi: 10.1128/jb.151.2.708-717.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Isenberg S., Newman E. B. Studies on L-serine deaminase in Escherichia coli K-12. J Bacteriol. 1974 Apr;118(1):53–58. doi: 10.1128/jb.118.1.53-58.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kawasaki T., Nakata T., Nose Y. Genetic mapping with a thiamine-requiring auxotroph of Escherichia coli K-12 defective in thiamine phosphate pyrophosphorylase. J Bacteriol. 1968 Apr;95(4):1483–1485. doi: 10.1128/jb.95.4.1483-1485.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MORRIS J. G., WOODS D. D. Inter-relationships of serine, glycine and vitamin B6 in the growth of mutants of Escherichia coli. J Gen Microbiol. 1959 Jun;20(3):576–596. doi: 10.1099/00221287-20-3-576. [DOI] [PubMed] [Google Scholar]
  10. Newman E. B., Adley T., Fraser J., Potter R., Kapoor V. The conversion of leucine to alpha-ketoisocaproic acid and its metabolic consequences for Escherichia coli K12. Can J Microbiol. 1976 Jul;22(7):922–928. doi: 10.1139/m76-133. [DOI] [PubMed] [Google Scholar]
  11. Newman E. B., Ahmad D., Walker C. L-Serine deaminase activity is induced by exposure of Escherichia coli K-12 to DNA-damaging agents. J Bacteriol. 1982 Nov;152(2):702–705. doi: 10.1128/jb.152.2.702-705.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Newman E. B., Kapoor V. In vitro studies on L-serine deaminase activity of Escherichia coli K12. Can J Biochem. 1980 Nov;58(11):1292–1297. doi: 10.1139/o80-173. [DOI] [PubMed] [Google Scholar]
  13. Newman E. B., Malik N., Walker C. L-serine degradation in Escherichia coli K-12: directly isolated ssd mutants and their intragenic revertants. J Bacteriol. 1982 May;150(2):710–715. doi: 10.1128/jb.150.2.710-715.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Newman E. B., Morris J. F., Walker C., Kapoor V. A mutation affecting L-serine and energy metabolism in E. coli K12. Mol Gen Genet. 1981;182(1):143–147. doi: 10.1007/BF00422781. [DOI] [PubMed] [Google Scholar]
  15. Newman E. B., Walker C. L-serine degradation in Escherichia coli K-12: a combination of L-serine, glycine, and leucine used as a source of carbon. J Bacteriol. 1982 Aug;151(2):777–782. doi: 10.1128/jb.151.2.777-782.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PARDEE A. B., PRESTIDGE L. S. Induced formation of serine and threonine deaminases by Escherichia coli. J Bacteriol. 1955 Dec;70(6):667–674. doi: 10.1128/jb.70.6.667-674.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ryals J., Hsu R. Y., Lipsett M. N., Bremer H. Isolation of single-site Escherichia coli mutants deficient in thiamine and 4-thiouridine syntheses: identification of a nuvC mutant. J Bacteriol. 1982 Aug;151(2):899–904. doi: 10.1128/jb.151.2.899-904.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shimizu S., Dempsey W. B. 3-hydroxypyruvate substitutes for pyridoxine in serC mutants of Escherichia coli K-12. J Bacteriol. 1978 Jun;134(3):944–949. doi: 10.1128/jb.134.3.944-949.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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