Abstract
The glucocorticoid hormones methyl prednisolone and dexamethasone were shown to be cytostatic, but not cytotoxic, at high cell densities for early passage and continuous cell lines from human glioma at 0.25 microM and above, in the presence or absence of serum. In the absence of serum both steroids at 2.5 nM increased the saturation density close to the level reached in serum. Examination of the iodinated glycoproteins of the cell surface by gel electrophoresis did not reveal any consistent change. However, gel exclusion chromatography of protease digests of the cell surface and of material released into the medium showed an increase in incorporation of 3H-glucosamine in pronase digests after treatment with methyl prednisolone. Ion exchange chromatography showed that sulphated glycosaminoglycans, particularly heparan sulphate, increased and hyaluronic acid decreased in response to steroids, and there was increased retention of GAGs on the cell surface relative to the released fraction. It was concluded that glucocorticoid hormones modify the cell surface of human glioma cells and that this may contribute to enhanced cell intraction and lead to increased density limitation of cell proliferation.
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- ABERCROMBIE M., HEAYSMAN J. E. Observations on the social behaviour of cells in tissue culture. II. Monolayering of fibroblasts. Exp Cell Res. 1954 May;6(2):293–306. doi: 10.1016/0014-4827(54)90176-7. [DOI] [PubMed] [Google Scholar]
- Frame M. C., Freshney R. I., Vaughan P. F., Graham D. I., Shaw R. Interrelationship between differentiation and malignancy-associated properties in glioma. Br J Cancer. 1984 Mar;49(3):269–280. doi: 10.1038/bjc.1984.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freshney R. I. Induction of differentiation in neoplastic cells. Anticancer Res. 1985 Jan-Feb;5(1):111–130. [PubMed] [Google Scholar]
- Freshney R. I. Tumour cells disaggregated in collagenase. Lancet. 1972 Sep 2;2(7775):488–489. doi: 10.1016/s0140-6736(72)91885-5. [DOI] [PubMed] [Google Scholar]
- Guner M., Freshney R. I., Morgan D., Freshney M. G., Thomas D. G., Graham D. I. Effects of dexamethasone and betamethasone on in vitro cultures from human astrocytoma. Br J Cancer. 1977 Apr;35(4):439–447. doi: 10.1038/bjc.1977.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guner M., Freshney R. I., Morgan D., Freshney M. G., Thomas D. G., Graham D. I. Effects of dexamethasone and betamethasone on in vitro cultures from human astrocytoma. Br J Cancer. 1977 Apr;35(4):439–447. doi: 10.1038/bjc.1977.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Li M. L., Aggeler J., Farson D. A., Hatier C., Hassell J., Bissell M. J. Influence of a reconstituted basement membrane and its components on casein gene expression and secretion in mouse mammary epithelial cells. Proc Natl Acad Sci U S A. 1987 Jan;84(1):136–140. doi: 10.1073/pnas.84.1.136. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McLean J. S., Frame M. C., Freshney R. I., Vaughan P. F., Mackie A. E., Singer I. Phenotypic modification of human glioma and non-small cell lung carcinoma by glucocorticoids and other agents. Anticancer Res. 1986 Sep-Oct;6(5):1101–1106. [PubMed] [Google Scholar]
- Walker M. J., Lim C., Das Gupta T. K., Beattie C. W. Effects of glucocorticoids on the growth of human fibrosarcoma cell line HT-1080. Cancer Res. 1986 Oct;46(10):4927–4932. [PubMed] [Google Scholar]