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. 1984 Jan;157(1):109–114. doi: 10.1128/jb.157.1.109-114.1984

Morphological and genetic characterization of a bacteriophage-resistant Bacillus subtilis macrofiber-producing strain.

C L Saxe 3rd, N H Mendelson
PMCID: PMC215137  PMID: 6418716

Abstract

Bacillus subtilis C6 phi R4 is an SPO1-resistant derivative of strain C6D, a left-hand macrofiber-producing strain described previously (N. H. Mendelson, Proc. Natl. Acad. Sci. U.S.A. 75:2478-2482, 1978). In addition to the phage resistance property, strain C6 phi R4 differs from its parent in macrofiber organization and formation of aggregates in liquid shake cultures. The phage resistance mutation was located in the gtaC gene. The macrofiber organization and aggregation phenotypes also appear to be controlled by the gtaC locus. Strains constructed by introduction of the gtaC mutation into C6D appear to be identical to the original C6 phi R4 strain in all phenotypic properties. In contrast, other constructs carrying either gtaA or gtaB that are resistant to SPO1 do not display the characteristic C6 phi R4 morphological phenotypes.

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Selected References

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  1. Boylan R. J., Mendelson N. H., Brooks D., Young F. E. Regulation of the bacterial cell wall: analysis of a mutant of Bacillus subtilis defective in biosynthesis of teichoic acid. J Bacteriol. 1972 Apr;110(1):281–290. doi: 10.1128/jb.110.1.281-290.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burge R. E., Fowler A. G., Reaveley D. A. Structure of the peptidogylcan of bacterial cell walls. I. J Mol Biol. 1977 Dec 25;117(4):927–953. doi: 10.1016/s0022-2836(77)80006-5. [DOI] [PubMed] [Google Scholar]
  3. Dedonder R. A., Lepesant J. A., Lepesant-Kejzlarová J., Billault A., Steinmetz M., Kunst F. Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168. Appl Environ Microbiol. 1977 Apr;33(4):989–993. doi: 10.1128/aem.33.4.989-993.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fein J. E. Helical growth and macrofiber formation of Bacillus subtilis 168 autolytic enzyme deficient mutants. Can J Microbiol. 1980 Mar;26(3):330–337. doi: 10.1139/m80-054. [DOI] [PubMed] [Google Scholar]
  5. Formanek H., Formanek S., Wawra H. A three-dimensional atomic model of the murein layer of bacteria. Eur J Biochem. 1974 Jul 15;46(2):279–294. doi: 10.1111/j.1432-1033.1974.tb03620.x. [DOI] [PubMed] [Google Scholar]
  6. Forsberg C. W., Wyrick P. B., Ward J. B., Rogers H. J. Effect of phosphate limitation on the morphology and wall composition of Bacillus licheniformis and its phosphoglucomutase-deficient mutants. J Bacteriol. 1973 Feb;113(2):969–984. doi: 10.1128/jb.113.2.969-984.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Frankel R. W., Joys T. M. Adsorption Specificity of Bacteriophage PBS1. J Bacteriol. 1966 Aug;92(2):388–389. doi: 10.1128/jb.92.2.388-389.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Henner D. J., Hoch J. A. The Bacillus subtilis chromosome. Microbiol Rev. 1980 Mar;44(1):57–82. doi: 10.1128/mr.44.1.57-82.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jacobson E. D., Landman O. E. Interaction of protoplasts, L forms, and bacilli of Bacillus subtilis with 12 strains of bacteriophage. J Bacteriol. 1975 Oct;124(1):445–448. doi: 10.1128/jb.124.1.445-448.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Koch A. L., Higgins M. L., Doyle R. J. The role of surface stress in the morphology of microbes. J Gen Microbiol. 1982 May;128(5):927–945. doi: 10.1099/00221287-128-5-927. [DOI] [PubMed] [Google Scholar]
  11. Lang W. K., Glassey K., Archibald A. R. Influence of phosphate supply on teichoic acid and teichuronic acid content of Bacillus subtilis cell walls. J Bacteriol. 1982 Jul;151(1):367–375. doi: 10.1128/jb.151.1.367-375.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Marquis R. E. Salt-induced contraction of bacterial cell walls. J Bacteriol. 1968 Mar;95(3):775–781. doi: 10.1128/jb.95.3.775-781.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mendelson N. H. Bacterial growth and division: genes, structures, forces, and clocks. Microbiol Rev. 1982 Sep;46(3):341–375. doi: 10.1128/mr.46.3.341-375.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mendelson N. H. Cell division suppression in the Bacillus subtilis div IC-A1 minicell-producing mutant. J Bacteriol. 1975 Mar;121(3):1166–1172. doi: 10.1128/jb.121.3.1166-1172.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mendelson N. H. Dynamics of Bacillus subtilis helical macrofiber morphogenesis: writhing, folding, close packing, and contraction. J Bacteriol. 1982 Jul;151(1):438–449. doi: 10.1128/jb.151.1.438-449.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mendelson N. H. Helical Bacillus subtilis macrofibers: morphogenesis of a bacterial multicellular macroorganism. Proc Natl Acad Sci U S A. 1978 May;75(5):2478–2482. doi: 10.1073/pnas.75.5.2478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mendelson N. H. Helical growth of Bacillus subtilis: a new model of cell growth. Proc Natl Acad Sci U S A. 1976 May;73(5):1740–1744. doi: 10.1073/pnas.73.5.1740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Norval M., Sutherland I. W. A group of klebsiella mutants showing temperature-dependent polysaccharide synthesis. J Gen Microbiol. 1969 Aug;57(3):369–377. doi: 10.1099/00221287-57-3-369. [DOI] [PubMed] [Google Scholar]
  19. Tilby M. J. Helical shape and wall synthesis in a bacterium. Nature. 1977 Mar 31;266(5601):450–452. doi: 10.1038/266450a0. [DOI] [PubMed] [Google Scholar]
  20. Yasbin R. E., Maino V. C., Young F. E. Bacteriophage resistance in Bacillus subtilis 168, W23, and interstrain transformants. J Bacteriol. 1976 Mar;125(3):1120–1126. doi: 10.1128/jb.125.3.1120-1126.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Young F. E. Requirement of glucosylated teichoic acid for adsorption of phage in Bacillus subtilis 168. Proc Natl Acad Sci U S A. 1967 Dec;58(6):2377–2384. doi: 10.1073/pnas.58.6.2377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Young F. E., Smith C., Reilly B. E. Chromosomal location of genes regulating resistance to bacteriophage in Bacillus subtilis. J Bacteriol. 1969 Jun;98(3):1087–1097. doi: 10.1128/jb.98.3.1087-1097.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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