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. 1984 Jan;157(1):122–125. doi: 10.1128/jb.157.1.122-125.1984

Role of quinones in the branch of the Escherichia coli respiratory chain that terminates in cytochrome o.

D C Au, G N Green, R B Gennis
PMCID: PMC215139  PMID: 6317645

Abstract

The role of quinones in the cytochrome o branch of the Escherichia coli respiratory chain was investigated by using mutant strains lacking the cytochrome d terminal oxidase complex. The only cytochromes present were cytochrome b556 and the cytochrome o complex, consisting of cytochrome b555-b562. Mutant strains missing ubiquinone, menaquinone, or both were constructed in the cytochrome d-minus (cyd) background. The steady-state levels of cytochrome b reduction were examined and compared in these strains to assess the effects of the quinone deficiencies. The data clearly show that a ubiquinone deficiency results in a lower level of cytochrome b reduction in the steady state. The data are consistent with a simple model in which ubiquinone is placed on the dehydrogenase side of all the cytochromes in this branch of the respiratory chain. There is no evidence from these experiments for a role of quinones in the respiratory chain at any site besides this one.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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