Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Jan;157(1):126–129. doi: 10.1128/jb.157.1.126-129.1984

Suppression by thymidine-requiring mutants of Escherichia coli K-12.

M B Herrington, A Kohli, P H Lapchak
PMCID: PMC215140  PMID: 6360992

Abstract

Thymidine-requiring strains of Escherichia coli isolated by trimethoprim selection often simultaneously acquire the ability to suppress bacteriophage T4 nonsense mutations. Suppression is lost in Thy+ revertants and recombinants, but is sometimes retained in thyA plasmid-bearing transformants. Suppression is restricted in Strr derivatives of the Thy- mutants, indicating that suppression occurs at the level of translation.

Full text

PDF
127

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alikhanian S. I., Iljina T. S., Kaliaeva E. S., Kameneva S. V., Sukhodolec V. V. A genetical study of thymineless mutants of E. coli K12. Genet Res. 1966 Aug;8(1):83–100. doi: 10.1017/s0016672300009939. [DOI] [PubMed] [Google Scholar]
  2. Apirion D. Altered ribosomes in a suppressor strain of Escherichia coli. J Mol Biol. 1966 Apr;16(2):285–301. doi: 10.1016/s0022-2836(66)80173-0. [DOI] [PubMed] [Google Scholar]
  3. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Belfort M., Maley G. F., Maley F. Characterization of the Escherichia coli thyA gene and its amplified thymidylate synthetase product. Proc Natl Acad Sci U S A. 1983 Apr;80(7):1858–1861. doi: 10.1073/pnas.80.7.1858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bertino J. B., Stacey K. A. A suggested mechanism for the selective procedure for isolating thymine-requiring mutants of Escherichia coli. Biochem J. 1966 Nov;101(2):32C–33C. doi: 10.1042/bj1010032c. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cheung P. K., Herrington M. B. Thymine inhibits suppression by an Escherichia coli nonsense and frameshift suppressor. Mol Gen Genet. 1982;186(2):217–220. doi: 10.1007/BF00331852. [DOI] [PubMed] [Google Scholar]
  7. Gesteland R. F. Isolation and characterization of ribonuclease I mutants of Escherichia coli. J Mol Biol. 1966 Mar;16(1):67–84. doi: 10.1016/s0022-2836(66)80263-2. [DOI] [PubMed] [Google Scholar]
  8. Hall B. G., Hartl D. L. Regulation of newly evolved enzymes. I. Selection of a novel lactase regulated by lactose in Escherichia coli. Genetics. 1974 Mar;76(3):391–400. doi: 10.1093/genetics/76.3.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Herrington M. B., Ganoza M. C. Genetic characterization of the temperature-sensitive and suppression phenotypes of Escherichia coli mutant N4316. J Bacteriol. 1977 Feb;129(2):1141–1143. doi: 10.1128/jb.129.2.1141-1143.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Herrington M. B., Lapchak P. H., Kohli A. Suppression by an Escherichia coli mutant under thymine-limiting conditions. Mutat Res. 1983 Feb;119(2):109–111. doi: 10.1016/0165-7992(83)90118-5. [DOI] [PubMed] [Google Scholar]
  11. Howe M. M. Prophage deletion mapping of bacteriophage Mu-1. Virology. 1973 Jul;54(1):93–101. doi: 10.1016/0042-6822(73)90118-9. [DOI] [PubMed] [Google Scholar]
  12. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  13. Phillips S. L., Schlessinger D., Apirion D. Temperature-dependent suppression of UGA and UAA codons in a temperature-sensitive mutant of Escherichia coli. Cold Spring Harb Symp Quant Biol. 1969;34:499–503. doi: 10.1101/sqb.1969.034.01.056. [DOI] [PubMed] [Google Scholar]
  14. Phoenix P., Melançon P., Brakier-Gingras L. Characterization of mutants of Escherichia coli with an increased control of translation fidelity. Mol Gen Genet. 1983;189(1):123–128. doi: 10.1007/BF00326064. [DOI] [PubMed] [Google Scholar]
  15. Roodman S. T., Greenberg G. R. A temperature-sensitive thy mutant blocked in the synthesis of thymidylate synthetase. J Biol Chem. 1971 Apr 25;246(8):2609–2617. [PubMed] [Google Scholar]
  16. Smith M. D., Green R. R., Ripley L. S., Drake J. W. Thymineless mutagenesis in bacteriophage T4. Genetics. 1973 Jul;74(3):393–403. doi: 10.1093/genetics/74.3.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Thompson R. C., Dix D. B., Gerson R. B., Karim A. M. Effect of Mg2+ concentration, polyamines, streptomycin, and mutations in ribosomal proteins on the accuracy of the two-step selection of aminoacyl-tRNAs in protein biosynthesis. J Biol Chem. 1981 Jul 10;256(13):6676–6681. [PubMed] [Google Scholar]
  18. Wilson M. C., Farmer J. L., Rothman F. Thymidylate synthesis and aminopterin resistance in Bacillus subtilis. J Bacteriol. 1966 Jul;92(1):186–196. doi: 10.1128/jb.92.1.186-196.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yates J. L. Role of ribosomal protein S12 in discrimination of aminoacyl-tRNA. J Biol Chem. 1979 Nov 25;254(22):11550–11554. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES