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. 1984 Jan;157(1):202–210. doi: 10.1128/jb.157.1.202-210.1984

Genetic analysis of a streptomycin-resistant oligosporogenous Bacillus subtilis mutant.

T M Henkin, G H Chambliss
PMCID: PMC215153  PMID: 6418717

Abstract

Strain SRB15T+, a streptomycin-resistant, oligosporogenous mutant of Bacillus subtilis, contains two mutations, fun and strR. These mutations were mapped by PBS-1 mediated transduction and by transformation to two different sites in the cysA-linked region of the B. subtilis chromosome. The fun mutation mapped very close to rpsLl, a classic strA mutation, whereas strR mapped to a site distal to rpsE. The effects of these mutations on growth, sporulation, and streptomycin resistance in vivo and in vitro were determined. The fun mutation gave a different phenotype than did the rpsLl mutation and caused altered migration of a ribosomal protein which was identified as S12, the protein encoded by rpsL. It therefore appears that fun is an allele of the rpsL gene.

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Selected References

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