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. 1984 Jan;157(1):303–310. doi: 10.1128/jb.157.1.303-310.1984

Isolation and analysis of aroFo mutants by using an aroF-lac operon fusion.

C S Cobbett, S Morrison, J Pittard
PMCID: PMC215167  PMID: 6317654

Abstract

The lac structural genes were fused to the regulatory region of the aroF-tyrA operon so that the expression of beta-galactosidase was regulated by the tyrR+ gene product. Transducing phage carrying the aroF-lac fusion were isolated, and a lambda aroF-lac lysogen was used to select for aroFo mutants. A plasmid vector was constructed onto which the aroFo mutations were transferred by recombination in vivo.

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Selected References

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