Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Feb;157(2):413–419. doi: 10.1128/jb.157.2.413-419.1984

Physical organization of the metJB component of the Escherichia coli K-12 metJBLF gene cluster.

C A Liljestrand-Golden, J R Johnson
PMCID: PMC215263  PMID: 6319357

Abstract

The structures of a series of plaque-forming metJB transducing phage were studied by restriction endonuclease mapping and enzyme activity assay. One of these phage, lambda pmet100, was inactivated by heat shock in the presence of EDTA, and deletion mutants were selected from the survivors. Two of these mutants, lambda pmet100 delta 1 and lambda pmet100 delta 2, were used to confirm the gene order metJ metB when moving clockwise on the linkage map of Escherichia coli K-12. Additional results indicate that the metB gene can be expressed independently of any other component of the met regulon and that the metJ gene also forms a separate transcription unit.

Full text

PDF
416

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adhya S., Gottesman M. Control of transcription termination. Annu Rev Biochem. 1978;47:967–996. doi: 10.1146/annurev.bi.47.070178.004535. [DOI] [PubMed] [Google Scholar]
  2. Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baksi K., Rogerson D. L., Rushizky G. W. Rapid, single-step purification of restriction endonucleases on cibacron blue F3GA-agarose. Biochemistry. 1978 Oct 3;17(20):4136–4139. doi: 10.1021/bi00613a005. [DOI] [PubMed] [Google Scholar]
  4. Daniels D. L., Blattner F. R. Nucleotide sequence of the Q gene and the Q to S intergenic region of bacteriophage lambda. Virology. 1982 Feb;117(1):81–92. doi: 10.1016/0042-6822(82)90509-8. [DOI] [PubMed] [Google Scholar]
  5. Greene R. C., Hunter J. S., Coch E. H. Properties of metK mutants of Escherichia coli K-12. J Bacteriol. 1973 Jul;115(1):57–67. doi: 10.1128/jb.115.1.57-67.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Greene R. C., Krueger J. H., Johnson J. R. Localization of the metJBLF gene cluster of Escherichia coli in lambda met transducing phage. Mol Gen Genet. 1982;187(3):401–404. doi: 10.1007/BF00332618. [DOI] [PubMed] [Google Scholar]
  7. Hammer-Jespersen K., Munch-Ptersen A. Multiple regulation of nucleoside catabolizing enzymes: regulation of the deo operon by the cytR and deoR gene products. Mol Gen Genet. 1975;137(4):327–335. doi: 10.1007/BF00703258. [DOI] [PubMed] [Google Scholar]
  8. Helling R. B., Goodman H. M., Boyer H. W. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol. 1974 Nov;14(5):1235–1244. doi: 10.1128/jvi.14.5.1235-1244.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Holloway C. T., Greene R. C., Su C. H. Regulation of S-adenosylmethionine synthetase in Escherichia coli. J Bacteriol. 1970 Nov;104(2):734–747. doi: 10.1128/jb.104.2.734-747.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johnson J. R., Greene R. C., Krueger J. H. Isolation and characterization of specialized lambda transducing bacteriophage carrying the metBJF methionine gene cluster. J Bacteriol. 1977 Sep;131(3):795–800. doi: 10.1128/jb.131.3.795-800.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Johnson J. R., Liljestrand C. A. Plaque assay for lambda transducing phage carrying the E. coli metB gene. Mol Gen Genet. 1983;190(3):527–530. doi: 10.1007/BF00331087. [DOI] [PubMed] [Google Scholar]
  12. Kraus J., Soll D., Low K. B. Glutamyl-gamma-methyl ester acts as a methionine analogue in Escherichia coli: analogue resistant mutants map at the metJ and metK loci. Genet Res. 1979 Feb;33(1):49–55. doi: 10.1017/s0016672300018152. [DOI] [PubMed] [Google Scholar]
  13. Krueger J. H., Johnson J. R., Greene R. C., Dresser M. Structural studies of lambda transducing bacteriophage carrying bacterial deoxyribonucleic acid from the metBJLF region of the Escherichia coli chromosome. J Bacteriol. 1981 Aug;147(2):612–621. doi: 10.1128/jb.147.2.612-621.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Mayer H., Reichenbach H. Restriction endonucleases: general survey procedure and survey of gliding bacteria. J Bacteriol. 1978 Nov;136(2):708–713. doi: 10.1128/jb.136.2.708-713.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mazaitis A. J., Palchaudhuri S., Glansdorff N., Maas W. K. Isolation and characterization of lambdadargECBH transducing phages and heteroduplex analysis of the argECBH cluster. Mol Gen Genet. 1976 Jan 16;143(2):185–196. doi: 10.1007/BF00266921. [DOI] [PubMed] [Google Scholar]
  17. McDonell M. W., Simon M. N., Studier F. W. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977 Feb 15;110(1):119–146. doi: 10.1016/s0022-2836(77)80102-2. [DOI] [PubMed] [Google Scholar]
  18. Minson A. C., Smith D. A. Methionine regulatory defects in Salmonella typhimurium arising from amber-suppressible mutations. J Gen Microbiol. 1972 May;70(3):471–476. doi: 10.1099/00221287-70-3-471. [DOI] [PubMed] [Google Scholar]
  19. Mulligan J. T., Margolin W., Krueger J. H., Walker G. C. Mutations affecting regulation of methionine biosynthetic genes isolated by use of met-lac fusions. J Bacteriol. 1982 Aug;151(2):609–619. doi: 10.1128/jb.151.2.609-619.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Parkinson J. S., Huskey R. J. Deletion mutants of bacteriophage lambda. I. Isolation and initial characterization. J Mol Biol. 1971 Mar 14;56(2):369–384. doi: 10.1016/0022-2836(71)90471-2. [DOI] [PubMed] [Google Scholar]
  21. Press R., Glansdorff N., Miner P., De Vries J., Kadner R., Maas W. K. Isolation of transducing particles of phi-80 bacteriophage that carry different regions of the Escherichia coli genome. Proc Natl Acad Sci U S A. 1971 Apr;68(4):795–798. doi: 10.1073/pnas.68.4.795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sanger F., Coulson A. R., Hong G. F., Hill D. F., Petersen G. B. Nucleotide sequence of bacteriophage lambda DNA. J Mol Biol. 1982 Dec 25;162(4):729–773. doi: 10.1016/0022-2836(82)90546-0. [DOI] [PubMed] [Google Scholar]
  23. Shimada K., Weisberg R. A., Gottesman M. E. Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J Mol Biol. 1972 Feb 14;63(3):483–503. doi: 10.1016/0022-2836(72)90443-3. [DOI] [PubMed] [Google Scholar]
  24. Su C. H., Greene R. C. Regulation of methionine biosynthesis in Escherichia coli: mapping of the metJ locus and properties of a metJ plus-metJ minus diploid. Proc Natl Acad Sci U S A. 1971 Feb;68(2):367–371. doi: 10.1073/pnas.68.2.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Zakin M. M., Duchange N., Ferrara P., Cohen G. N. Nucleotide sequence of the metL gene of Escherichia coli. Its product, the bifunctional aspartokinase ii-homoserine dehydrogenase II, and the bifunctional product of the thrA gene, aspartokinase I-homoserine dehydrogenase I, derive from a common ancestor. J Biol Chem. 1983 Mar 10;258(5):3028–3031. [PubMed] [Google Scholar]
  26. Zakin M. M., Greene R. C., Dautry-Varsat A., Duchange N., Ferrara P., Py M. C., Margarita D., Cohen G. N. Construction and physical mapping of plasmids containing the metJBLF gene cluster of E. coli K12. Mol Gen Genet. 1982;187(1):101–106. doi: 10.1007/BF00384390. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES