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. 1984 Feb;157(2):526–532. doi: 10.1128/jb.157.2.526-532.1984

Characterization of the cloned fip gene and its product.

M Russel, P Model
PMCID: PMC215278  PMID: 6319363

Abstract

A DNA fragment encoding the fip (filamentous phage production) gene from Escherichia coli, when cloned in a filamentous phage vector, restored to the phage ability to assemble progeny in fip mutant hosts. The fip gene was located just upstream of and transcribed in the same direction as the rho gene. Minicells containing fip+ phage or plasmids synthesized a 12,500-dalton protein that was missing or truncated when the Fip+ phenotype was inactivated by insertion of Tn5. The fip protein was cytoplasmic and was partially purified.

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Selected References

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  1. Adler H. I., Fisher W. D., Cohen A., Hardigree A. A. MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967 Feb;57(2):321–326. doi: 10.1073/pnas.57.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bolivar F. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules. Gene. 1978 Oct;4(2):121–136. doi: 10.1016/0378-1119(78)90025-2. [DOI] [PubMed] [Google Scholar]
  3. Brown S., Albrechtsen B., Pedersen S., Klemm P. Localization and regulation of the structural gene for transcription-termination factor rho of Escherichia coli. J Mol Biol. 1982 Dec 5;162(2):283–298. doi: 10.1016/0022-2836(82)90527-7. [DOI] [PubMed] [Google Scholar]
  4. Casjens S., King J. Virus assembly. Annu Rev Biochem. 1975;44:555–611. doi: 10.1146/annurev.bi.44.070175.003011. [DOI] [PubMed] [Google Scholar]
  5. Davis R. W., Thomas M., Cameron J., St John T. P., Scherer S., Padgett R. A. Rapid DNA isolations for enzymatic and hybridization analysis. Methods Enzymol. 1980;65(1):404–411. doi: 10.1016/s0076-6879(80)65051-4. [DOI] [PubMed] [Google Scholar]
  6. HOFFMANN-BERLING H., DUERWALD H., BEULKE I. EIN FAEDIGER DNS-PHAGE (FD) UND EIN SPHAERISCHER RNS-PHAGE (FR) WIRTSSPEZIFISCH FUER MAENNLICHE STAEMME VON E. COLI. III. BIOLOGISCHES VERHALTEN VON FD UND FR. Z Naturforsch B. 1963 Nov;18:893–898. [PubMed] [Google Scholar]
  7. Hirashima A., Childs G., Inouye M. Differential inhibitory effects of antibiotics on the biosynthesis of envelope proteins of Escherichia coli. J Mol Biol. 1973 Sep 15;79(2):373–389. doi: 10.1016/0022-2836(73)90012-0. [DOI] [PubMed] [Google Scholar]
  8. Ito K., Mandel G., Wickner W. Soluble precursor of an integral membrane protein: synthesis of procoat protein in Escherichia coli infected with bacteriophage M13. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1199–1203. doi: 10.1073/pnas.76.3.1199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jorgensen R. A., Rothstein S. J., Reznikoff W. S. A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Mol Gen Genet. 1979;177(1):65–72. doi: 10.1007/BF00267254. [DOI] [PubMed] [Google Scholar]
  10. Kaguni J., Ray D. S. Cloning of a functional replication origin of phage G4 into the genome of phage M13. J Mol Biol. 1979 Dec 25;135(4):863–878. doi: 10.1016/0022-2836(79)90516-3. [DOI] [PubMed] [Google Scholar]
  11. Kleckner N., Chan R. K., Tye B. K., Botstein D. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J Mol Biol. 1975 Oct 5;97(4):561–575. doi: 10.1016/s0022-2836(75)80059-3. [DOI] [PubMed] [Google Scholar]
  12. Lin N. S., Pratt D. Bacteriophage M 13 gene 2 protein: increasing its yield in infected cells, and identification and localization. Virology. 1974 Oct;61(2):334–342. doi: 10.1016/0042-6822(74)90271-2. [DOI] [PubMed] [Google Scholar]
  13. Lopez J., Webster R. E. Morphogenesis of filamentous bacteriophage f1: orientation of extrusion and production of polyphage. Virology. 1983 May;127(1):177–193. doi: 10.1016/0042-6822(83)90382-3. [DOI] [PubMed] [Google Scholar]
  14. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  15. Nakamura K., Inouye M. Construction of versatile expression cloning vehicles using the lipoprotein gene of Escherichia coli. EMBO J. 1982;1(6):771–775. doi: 10.1002/j.1460-2075.1982.tb01244.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ohkawa I., Webster R. E. The orientation of the major coat protein of bacteriophage f1 in the cytoplasmic membrane of Escherichia coli. J Biol Chem. 1981 Oct 10;256(19):9951–9958. [PubMed] [Google Scholar]
  17. Ohta N., Chen L. S., Newton A. Isolation and expression of cloned hook protein gene from Caulobacter crescentus. Proc Natl Acad Sci U S A. 1982 Aug;79(16):4863–4867. doi: 10.1073/pnas.79.16.4863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pinkham J. L., Platt T. The nucleotide sequence of the rho gene of E. coli K-12. Nucleic Acids Res. 1983 Jun 11;11(11):3531–3545. doi: 10.1093/nar/11.11.3531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Russel M., Model P. A bacterial gene, fip, required for filamentous bacteriophage fl assembly. J Bacteriol. 1983 Jun;154(3):1064–1076. doi: 10.1128/jb.154.3.1064-1076.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Russel M., Model P. A mutation downstream from the signal peptidase cleavage site affects cleavage but not membrane insertion of phage coat protein. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1717–1721. doi: 10.1073/pnas.78.3.1717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shaw W. V., Packman L. C., Burleigh B. D., Dell A., Morris H. R., Hartley B. S. Primary structure of a chloramphenicol acetyltransferase specified by R plasmids. Nature. 1979 Dec 20;282(5741):870–872. doi: 10.1038/282870a0. [DOI] [PubMed] [Google Scholar]
  22. Sutcliffe J. G. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737–3741. doi: 10.1073/pnas.75.8.3737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tessman I., Fassler J. S., Bennett D. C. Relative map location of the rep and rho genes of Escherichia coli. J Bacteriol. 1982 Sep;151(3):1637–1640. doi: 10.1128/jb.151.3.1637-1640.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  25. Zinder N. D., Boeke J. D. The filamentous phage (Ff) as vectors for recombinant DNA--a review. Gene. 1982 Jul-Aug;19(1):1–10. doi: 10.1016/0378-1119(82)90183-4. [DOI] [PubMed] [Google Scholar]

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