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. 1984 Apr;158(1):63–68. doi: 10.1128/jb.158.1.63-68.1984

Rapid turnover of mannitol-1-phosphate in Escherichia coli.

H Rosenberg, S M Pearce, C M Hardy, P A Jacomb
PMCID: PMC215379  PMID: 6425270

Abstract

The phosphate moiety of D-mannitol-1-phosphate in Escherichia coli is subject to rapid turnover and is in close equilibrium with Pi and the phosphorus of fructose-1,6-bisphosphate. These three compounds account for the bulk of 32P label found in cells after several minutes of uptake of 32Pi and mannitol-1-phosphate represents some 30% of this label. Mannitol-1-phosphate occurs in E. coli grown on a variety of carbon sources, in the absence of D-mannitol, and is synthesized de novo even in mutants lacking mannitol-1-phosphate dehydrogenase. The mannitol moiety of mannitol-1-phosphate was not affected during the total chase of the P moiety, which exchanged with a half-life of about 30 s. These findings suggest that the rapid equilibration of the phosphorus is a function of an enzyme, possibly a component of the phosphotransferase system, capable of forming a complex that allows the exchange of the phosphate without the equilibration of the mannitol moiety with free mannitol.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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