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. 1984 Dec;160(3):884–888. doi: 10.1128/jb.160.3.884-888.1984

Sensitivity to nikkomycin Z in Candida albicans: role of peptide permeases.

J C Yadan, M Gonneau, P Sarthou, F Le Goffic
PMCID: PMC215792  PMID: 6389515

Abstract

The uptake of tritiated nikkomycin Z, a potent inhibitor of chitin synthetase, is mediated by a peptide transport system in Candida albicans. Kinetic transport assays with radioactive di- and tripeptides and competition studies suggest that two distinct systems operate in this yeast. Nikkomycin Z was transported through one of these systems, common to di- and tripeptides. A peptide transport-deficient mutant was isolated on the basis of its resistance to nikkomycin Z. The mutant lost most of its capacity to take up dipeptides but simultaneously increased its ability to transport tripeptides. These results indicate that C. albicans handles peptides through multiple transport systems and adjusts their expression to environmental conditions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames B. N., Ames G. F., Young J. D., Tsuchiya D., Lecocq J. Illicit transport: the oligopeptide permease. Proc Natl Acad Sci U S A. 1973 Feb;70(2):456–458. doi: 10.1073/pnas.70.2.456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Atherton F. R., Hall M. J., Hassall C. H., Lambert R. W., Lloyd W. J., Lord A. V., Ringrose P. S., Westmacott D. Phosphonopeptides as substrates for peptide transport systems and peptidases of Escherichia coli. Antimicrob Agents Chemother. 1983 Oct;24(4):522–528. doi: 10.1128/aac.24.4.522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Becker J. M., Covert N. L., Shenbagamurthi P., Steinfeld A. S., Naider F. Polyoxin D inhibits growth of zoopathogenic fungi. Antimicrob Agents Chemother. 1983 Jun;23(6):926–929. doi: 10.1128/aac.23.6.926. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boehm J. C., Kingsbury W. D., Perry D., Gilvarg C. The use of cysteinyl peptides to effect portage transport of sulfhydryl-containing compounds in Escherichia coli. J Biol Chem. 1983 Dec 25;258(24):14850–14855. [PubMed] [Google Scholar]
  5. Davies M. B. Peptide uptake in Candida albicans. J Gen Microbiol. 1980 Nov;121(1):181–186. doi: 10.1099/00221287-121-1-181. [DOI] [PubMed] [Google Scholar]
  6. Higgins C. F., Hardie M. M. Periplasmic protein associated with the oligopeptide permeases of Salmonella typhimurium and Escherichia coli. J Bacteriol. 1983 Sep;155(3):1434–1438. doi: 10.1128/jb.155.3.1434-1438.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kenig M., Vandamme E., Abraham E. P. The mode of action of bacilysin and anticapsin and biochemical properties of bacilysin-resistant mutants. J Gen Microbiol. 1976 May;94(1):46–54. doi: 10.1099/00221287-94-1-46. [DOI] [PubMed] [Google Scholar]
  8. Kingsbury W. D., Boehm J. C., Mehta R. J., Grappel S. F. Transport of antimicrobial agents using peptide carrier systems: anticandidal activity of m-fluorophenylalanine--peptide conjugates. J Med Chem. 1983 Dec;26(12):1725–1729. doi: 10.1021/jm00366a013. [DOI] [PubMed] [Google Scholar]
  9. Lichliter W. D., Naider F., Becker J. M. Basis for the design of anticandidal agents from studies of peptide utilization in Canadida albicans. Antimicrob Agents Chemother. 1976 Sep;10(3):483–490. doi: 10.1128/aac.10.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Logan D. A., Becker J. M., Naider F. Peptide transport in Candida albicans. J Gen Microbiol. 1979 Sep;114(1):179–186. doi: 10.1099/00221287-114-1-179. [DOI] [PubMed] [Google Scholar]
  11. Mitani M., Inoue Y. Antagonists of an antifungal substance, polyoxin. J Antibiot (Tokyo) 1968 Aug;21(8):492–496. doi: 10.7164/antibiotics.21.492. [DOI] [PubMed] [Google Scholar]
  12. Naider F., Shenbagamurthi P., Steinfeld A. S., Smith H. A., Boney C., Becker J. M. Synthesis and biological activity of tripeptidyl polyoxins as antifungal agents. Antimicrob Agents Chemother. 1983 Nov;24(5):787–796. doi: 10.1128/aac.24.5.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sarthou P., Gonneau M., Le Goffic F. Photoaffinity inhibition of peptide transport in yeast. Biochem Biophys Res Commun. 1983 Feb 10;110(3):884–889. doi: 10.1016/0006-291x(83)91044-6. [DOI] [PubMed] [Google Scholar]
  14. Shenbagamurthi P., Smith H. A., Becker J. M., Steinfeld A., Naider F. Design of anticandidal agents: synthesis and biological properties of analogues of polyoxin L. J Med Chem. 1983 Oct;26(10):1518–1522. doi: 10.1021/jm00364a030. [DOI] [PubMed] [Google Scholar]
  15. Ti J. S., Steinfeld A. S., Naider F., Gulumoglu A., Lewis S. V., Becker J. M. Anticandidal activity of pyrimidine-peptide conjugates. J Med Chem. 1980 Aug;23(8):913–918. doi: 10.1021/jm00182a019. [DOI] [PubMed] [Google Scholar]

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