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. 1981 Jul;147(1):155–160. doi: 10.1128/jb.147.1.155-160.1981

Intergeneric transfer of deoxyribonucleic acid killer plasmids, pGKl1 and pGKl2, from Kluyveromyces lactis into Saccharomyces cerevisiae by cell fusion.

N Gunge, K Sakaguchi
PMCID: PMC216019  PMID: 7016841

Abstract

Two novel linear deoxyribonucleic acid plasmids, pGKl1 and pGKl2, were isolated from the yeast Kluyveromyces lactis. K. lactis strains harboring the pGK1 plasmids killed a certain group of yeasts, including Saccharomyces cerevisiae, Saccharomyces italicus, Saccharomyces rouxii, K. lactis, Kluyveromyces thermotolerans, Kluyvermyces vanudenii, Torulopsis glabrata, Candida utilis, and Candida intermedia. In this experiment, the pGKl1 and pGKl2 plasmids were intergenerically transferred from a K. lactis killer strain into a non-killer (killer-sensitive) strain of S. cerevisiae by the use of a protoplast fusion technique. Both of the pGKl plasmids replicated autonomously and stably in the new host cells of S. cerevisiae and could coexist with the resident 2-micrometers deoxyribonucleic acid plasmid. The S. cerevisiae cells which accepted the pGKl plasmids expressed the same killer phenotype as that of the donor K. lactis killer and became resistant to the K. lactis killer. The pGKl plasmids existing in the S. cerevisiae cells were cured by treatment with ethidium bromide, and the killer and resistance characters were simultaneously lost. From there results, it was concluded that both the killer and the resistance genes are located on the pGKl plasmids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beggs J. D. Transformation of yeast by a replicating hybrid plasmid. Nature. 1978 Sep 14;275(5676):104–109. doi: 10.1038/275104a0. [DOI] [PubMed] [Google Scholar]
  2. Bevan E. A., Herring A. J., Mitchell D. J. Preliminary characterization of two species of dsRNA in yeast and their relationship to the "killer" character. Nature. 1973 Sep 14;245(5420):81–86. doi: 10.1038/245081b0. [DOI] [PubMed] [Google Scholar]
  3. Bostian K. A., Hopper J. E., Rogers D. T., Tipper D. J. Translational analysis of the killer-associated virus-like particle dsRNA genome of S. cerevisiae: M dsRNA encodes toxin. Cell. 1980 Feb;19(2):403–414. doi: 10.1016/0092-8674(80)90514-0. [DOI] [PubMed] [Google Scholar]
  4. Bussey H., Skipper N. Killing of Torulopsis glabrata by Saccharomyces cerevisiae killer factor. Antimicrob Agents Chemother. 1976 Feb;9(2):352–354. doi: 10.1128/aac.9.2.352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cameron J. R., Philippsen P., Davis R. W. Analysis of chromosomal integration and deletions of yeast plasmids. Nucleic Acids Res. 1977;4(5):1429–1448. doi: 10.1093/nar/4.5.1429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clark-Walker G. D. Isolation of circular DNA from a mitochondrial fraction from yeast. Proc Natl Acad Sci U S A. 1972 Feb;69(2):388–392. doi: 10.1073/pnas.69.2.388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Clark-Walker G. D., Miklos G. L. Localization and quantification of circular DNA in yeast. Eur J Biochem. 1974 Jan 16;41(2):359–365. doi: 10.1111/j.1432-1033.1974.tb03278.x. [DOI] [PubMed] [Google Scholar]
  8. Ferenczy L., Maráz A. Transfer of mitochondria by protoplast fusion in Saccharomyces cerevisiae. Nature. 1977 Aug 11;268(5620):524–525. doi: 10.1038/268524a0. [DOI] [PubMed] [Google Scholar]
  9. Fink G. R., Styles C. A. Curing of a killer factor in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2846–2849. doi: 10.1073/pnas.69.10.2846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fukuda H., Kimura A. Transfer of mitochondria into protoplasts of Saccharomyces cerevisiae by mini-protoplast fusion. FEBS Lett. 1980 Apr 21;113(1):58–60. doi: 10.1016/0014-5793(80)80494-7. [DOI] [PubMed] [Google Scholar]
  11. Gerbaud C., Fournier P., Blanc H., Aigle M., Heslot H., Guerineau M. High frequency of yeast transformation by plasmids carrying part or entire 2-micron yeast plasmid. Gene. 1979 Mar;5(3):233–253. doi: 10.1016/0378-1119(79)90080-5. [DOI] [PubMed] [Google Scholar]
  12. Griffiths D. E., Lancashire W. E., Zanders E. D. Evidence for an extra-chromosomal element involved in mitochondrial function: a mitochondrial episome? FEBS Lett. 1975 May 1;53(2):126–130. doi: 10.1016/0014-5793(75)80002-0. [DOI] [PubMed] [Google Scholar]
  13. Guerineau M., Grandchamp C., Paoletti C., Slonimski P. Characterization of a new class of circular DNA molecules in yeast. Biochem Biophys Res Commun. 1971 Feb 5;42(3):550–557. doi: 10.1016/0006-291x(71)90406-2. [DOI] [PubMed] [Google Scholar]
  14. Guerineau M., Slonimski P. P., Avner P. R. Yeast episome: oligomycin resistance associated with a small covalently closed non-mitochondrial circular DNA. Biochem Biophys Res Commun. 1974 Nov 27;61(2):462–469. doi: 10.1016/0006-291x(74)90979-6. [DOI] [PubMed] [Google Scholar]
  15. Gunge N., Sakaguchi K. Fusion of mitochondria with protoplasts in Saccharomyces cerevisiae. Mol Gen Genet. 1979 Mar 5;170(3):243–247. doi: 10.1007/BF00267057. [DOI] [PubMed] [Google Scholar]
  16. Gunge N., Tamaru A., Ozawa F., Sakaguchi K. Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character. J Bacteriol. 1981 Jan;145(1):382–390. doi: 10.1128/jb.145.1.382-390.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Herring A. J., Bevan E. A. Virus-like particles associated with the double-stranded RNA species found in killer and sensitive strains of the yeast Saccharomyces cerevisiae. J Gen Virol. 1974 Mar;22(3):387–394. doi: 10.1099/0022-1317-22-3-387. [DOI] [PubMed] [Google Scholar]
  18. Hopper J. E., Bostian K. A., Rowe L. B., Tipper D. J. Translation of the L-species dsRNA genome of the killer-associated virus-like particles of Saccharomyces cerevisiae. J Biol Chem. 1977 Dec 25;252(24):9010–9017. [PubMed] [Google Scholar]
  19. Kingsman A. J., Clarke L., Mortimer R. K., Carbon J. Replication in Saccharomyces cerevisiae of plasmid pBR313 carrying DNA from the yeast trpl region. Gene. 1979 Oct;7(2):141–152. doi: 10.1016/0378-1119(79)90029-5. [DOI] [PubMed] [Google Scholar]
  20. Livingston D. M. Inheritance of the 2 micrometer m DNA plasmid from Saccharomyces. Genetics. 1977 May;86(1):73–84. doi: 10.1093/genetics/86.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Oliver S. G., McCREADY S. J., Holm C., Sutherland P. A., McLaughlin C. S., Cox B. S. Biochemical and physiological studies of the yeast virus-like particle. J Bacteriol. 1977 Jun;130(3):1303–1309. doi: 10.1128/jb.130.3.1303-1309.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Struhl K., Stinchcomb D. T., Scherer S., Davis R. W. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035–1039. doi: 10.1073/pnas.76.3.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sweeney T. K., Tate A., Fink G. R. A study of the transmission and structure of double stranded RNAs associated with the killer phenomenon in Saccharomyces cerevisiae. Genetics. 1976 Sep;84(1):27–42. doi: 10.1093/genetics/84.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Vodkin M. Induction of yeast killer factor mutations. J Bacteriol. 1977 Oct;132(1):346–348. doi: 10.1128/jb.132.1.346-348.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vodkin M., Katterman F., Fink G. R. Yeast killer mutants with altered double-stranded ribonucleic acid. J Bacteriol. 1974 Feb;117(2):681–686. doi: 10.1128/jb.117.2.681-686.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wickner R. B. Killer of Saccharomyces cerevisiae: a double-stranded ribonucleic acid plasmid. Bacteriol Rev. 1976 Sep;40(3):757–773. doi: 10.1128/br.40.3.757-773.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wickner R. B. The killer double-stranded RNA plasmids of yeast. Plasmid. 1979 Jul;2(3):303–322. doi: 10.1016/0147-619x(79)90015-5. [DOI] [PubMed] [Google Scholar]

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