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. 1981 Sep;147(3):736–743. doi: 10.1128/jb.147.3.736-743.1981

Complementation of replication-deficient deletion derivatives of plasmid mini-F.

J Ebbers, R Eichenlaub
PMCID: PMC216108  PMID: 6268604

Abstract

Deleted mini-F plasmids with defects in replication were constructed and tested to see whether they could be rescued through complementation by a helper plasmid. This allowed us to identify two genetic loci determining trans-acting functions required for stable maintenance of plasmid mini-F, one encoded by the PstI fragment from 45.7 to 47.3 F-coordinates (F) and the other most probably located in the region from 43.1 to 43.8 F. The smallest mini-F plasmid that could be established through complementation consists of the PstI fragment 44.0 to 45.7 F, encoding origin II and the incB locus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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