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. 1981 Oct;148(1):124–130. doi: 10.1128/jb.148.1.124-130.1981

Restriction map of corynebacteriophages beta c and beta vir and physical localization of the diphtheria tox operon.

J J Costa, J L Michel, R Rappuoli, J R Murphy
PMCID: PMC216174  PMID: 6270058

Abstract

The BamHI, EcoRI, HindIII, and KpnI restriction endonuclease maps of corynebacteriophage beta c and beta vir were constructed. beta vir appeared to be identical to beta c, except for an approximate 1-kilobase deletion that removed a BamHI site, two KpnI sites, and three EcoRI restriction sites. The diphtheria tox operon was located by hybridizing in vitro 32P-labeled tox messenger ribonucleic acid to blots of endonuclease-digested beta deoxyribonucleic acids. The messenger ribonucleic acid probe was found to hybridize to a 2.1-kilobase region of the beta genome. Since approximately 1.9 kilobases is required to encode prodiphtheria toxin, the data presented strongly suggest that the tox operon of beta is monocistronic.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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