Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Nov;148(2):739–743. doi: 10.1128/jb.148.2.739-743.1981

Effects of recB21, recF143, and uvrD152 on recombination in lambda bacteriophage-prophage and Hfr by F- crosses.

P Howard-Flanders, E Bardwell
PMCID: PMC216266  PMID: 6457825

Abstract

The effects of the mutation pairs recB21 recF143 and recB21 uvrD152 on the frequency of genetic recombination were investigated in lambda phage-prophage crosses under homoimmune conditions. To prevent recombinants from being formed by the phage red system, these experiments were performed with phages and prophages carrying red and gam mutations. Both spontaneous and damage-induced recombination was measured, the phages being either undamaged or treated with trimethylpsoralen and 360-nm light to cross-link the phage DNA. Control and damaged phages were allowed to infect lysogenic host cells under conditions in which phage gene expression was repressed and phage DNA replication was blocked by lambda immunity. Although the double mutations recB21 recF143 and recB21 uvrD152 reduced recombination in Hfr by F- crosses to 0.3 to 0.02% of the wild-type controls, the presence of these pairs of mutations in the host lysogens had relatively little effect on the results of the phage-prophage crosses. In the latter system, recB21 recF143 reduced spontaneous and damaged-induced recombination by less than threefold whereas recB21 uvrD152 increased it to three times the wild-type level, the increase being attributable to the uvrD mutation. Evidently, the gene products of recB,C uvrD, and recF wee not needed for lambda phage-prophage recombination under repressed conditions.

Full text

PDF
742

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birge E. A., Low K. B. Detection of transcribable recombination products following conjugation in rec+, reCB- and recC-strains of Escherichia coli K12. J Mol Biol. 1974 Mar 15;83(4):447–457. doi: 10.1016/0022-2836(74)90506-3. [DOI] [PubMed] [Google Scholar]
  3. Clark A. J. The beginning of a genetic analysis of recombination proficiency. J Cell Physiol. 1967 Oct;70(2 Suppl):165–180. doi: 10.1002/jcp.1040700412. [DOI] [PubMed] [Google Scholar]
  4. Clowes R. C., Moody E. E. Chromosomal transfer from "recombination-deficient" strains of Escherichia coli K-12. Genetics. 1966 Apr;53(4):717–726. doi: 10.1093/genetics/53.4.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hays J. B., Boehmer S. Antagonists of DNA gyrase inhibit repair and recombination of UV-irradiated phage lambda. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4125–4129. doi: 10.1073/pnas.75.9.4125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Horii Z., Clark A. J. Genetic analysis of the recF pathway to genetic recombination in Escherichia coli K12: isolation and characterization of mutants. J Mol Biol. 1973 Oct 25;80(2):327–344. doi: 10.1016/0022-2836(73)90176-9. [DOI] [PubMed] [Google Scholar]
  7. Howard-Flanders P., Rupp W. D., Wilkins B. M., Cole R. S. DNA replication and recombination after UV irradiation. Cold Spring Harb Symp Quant Biol. 1968;33:195–207. doi: 10.1101/sqb.1968.033.01.023. [DOI] [PubMed] [Google Scholar]
  8. Lin P. F., Bardwell E., Howard-Flanders P. Initiation of genetic exchanges in lambda phage--prophage crosses. Proc Natl Acad Sci U S A. 1977 Jan;74(1):291–295. doi: 10.1073/pnas.74.1.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Low B. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A. 1968 May;60(1):160–167. doi: 10.1073/pnas.60.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Porter R. D., McLaughlin T., Low B. Transduction versus "conjuduction": evidence for multiple roles for exonuclease V in genetic recombination in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1043–1047. doi: 10.1101/sqb.1979.043.01.113. [DOI] [PubMed] [Google Scholar]
  11. Rosner J. L. Formation, induction, and curing of bacteriophage P1 lysogens. Virology. 1972 Jun;48(3):679–689. doi: 10.1016/0042-6822(72)90152-3. [DOI] [PubMed] [Google Scholar]
  12. Rothman R. H., Clark A. J. Defective excision and postreplication repair of UV-damaged DNA in a recL mutant strain of E. coli K-12. Mol Gen Genet. 1977 Oct 24;155(3):267–277. doi: 10.1007/BF00272805. [DOI] [PubMed] [Google Scholar]
  13. Rothman R. H., Clark A. J. The dependence of postreplication repair on uvrB in a recF mutant of Escherichia coli K-12. Mol Gen Genet. 1977 Oct 24;155(3):279–286. doi: 10.1007/BF00272806. [DOI] [PubMed] [Google Scholar]
  14. Wilkins B. M. Chromosome transfer from F-lac+ strains of Escherichia coli K-12 mutant at recA, recB, or recC. J Bacteriol. 1969 May;98(2):599–604. doi: 10.1128/jb.98.2.599-604.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Zieg J., Maples V. F., Kushner S. R. Recombinant levels of Escherichia coli K-12 mutants deficient in various replication, recombination, or repair genes. J Bacteriol. 1978 Jun;134(3):958–966. doi: 10.1128/jb.134.3.958-966.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES