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. 1981 Dec;148(3):753–761. doi: 10.1128/jb.148.3.753-761.1981

Isolation and characterization of an Escherichia coli mutant affected in the regulation of adenylate cyclase.

C Guidi-Rontani, A Danchin, A Ullmann
PMCID: PMC216272  PMID: 6273380

Abstract

A mutant, cyaR1, affecting regulation of adenylate cyclase expression or activity is described. It was obtained as a thermoresistant revertant of a strain harboring a thermosensitive transcription termination factor, rho (rho-15). This mutant failed to synthesize adenosine 3',5'-phosphate and exhibited a carbohydrate-negative phenotype. A secondary mutation at the crp locus (crpC) restored the ability of the mutant to synthesize adenosine 3',5'-phosphate, enabled the expression of catabolite-sensitive operons, and conferred on the strain an extreme sensitivity to catabolite repression. In addition, we showed that the crpC mutation restored the pleiotropic carbohydrate-positive phenotype even in a delta cya background. We interpret this to mean that the adenosine 3',5'-phosphate receptor protein regulates negatively either the activity or synthesis of adenylate cyclase and that the cyaR1 mutation is either in a regulatory protein or a regulatory site of adenylate cyclase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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