Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Dec;148(3):1002–1005. doi: 10.1128/jb.148.3.1002-1005.1981

Regulation of glutamate dehydrogenase in Bacillus subtilis.

J F Kane, J Wakim, R S Fischer
PMCID: PMC216307  PMID: 6118356

Abstract

The activity of the nicotinamide adenine dinucleotide-dependent glutamate dehydrogenase in Bacillus subtilis was influenced by the carbon source, but not the nitrogen source, in the growth medium. The highest specific activity for this enzyme was found when B. subtilis was grown in a minimal or rich medium that contained glutamate as the carbon source. It is proposed that glutamate dehydrogenase serves a catabolic function in the metabolism of glutamate, is induced by glutamate, and is subject to catabolite repression.

Full text

PDF
1004

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bender R. A., Macaluso A., Magasanik B. Glutamate dehydrogenase: genetic mapping and isolation of regulatory mutants of Klebsiella aerogenes. J Bacteriol. 1976 Jul;127(1):141–148. doi: 10.1128/jb.127.1.141-148.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brenchley J. E., Baker C. A., Patil L. G. Regulation of the ammonia assimilatory enzymes in Salmonella typhimurium. J Bacteriol. 1975 Oct;124(1):182–189. doi: 10.1128/jb.124.1.182-189.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brenchley J. E., Magasanik B. Mutants of Klebsiella aerogenes lacking glutamate dehydrogenase. J Bacteriol. 1974 Feb;117(2):544–550. doi: 10.1128/jb.117.2.544-550.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Charba J. F., Nakata H. M. Role of glutamate in the sporogenesis of Bacillus cereus. J Bacteriol. 1977 Apr;130(1):242–248. doi: 10.1128/jb.130.1.242-248.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dendinger S. M., Brenchley J. E. Temperature-sensitive glutamate dehydrogenase mutants of Salmonella typhimurium. J Bacteriol. 1980 Dec;144(3):1043–1047. doi: 10.1128/jb.144.3.1043-1047.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dendinger S. M., Patil L. G., Brenchley J. E. Salmonella typhimurium mutants with altered glutamate dehydrogenase and glutamate synthase activities. J Bacteriol. 1980 Jan;141(1):190–198. doi: 10.1128/jb.141.1.190-198.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Deshpande K. L., Katze J. R., Kane J. F. Effect of glutamine on enzymes of nitrogen metabolism in Bacillus subtilis. J Bacteriol. 1981 Feb;145(2):768–774. doi: 10.1128/jb.145.2.768-774.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hemmilä I. A., Mäntsälä P. I. Purification and properties of glutamate synthase and glutamate dehydrogenase from Bacillus megaterium. Biochem J. 1978 Jul 1;173(1):45–52. doi: 10.1042/bj1730045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hemmings B. A., Sims A. P. The regulation of glutamate metabolism in Candida utilis. Evidence for two interconvertible forms of NAD-dependent glutamate dehydrogenase. Eur J Biochem. 1977 Oct 17;80(1):143–151. doi: 10.1111/j.1432-1033.1977.tb11866.x. [DOI] [PubMed] [Google Scholar]
  10. Kane J. F., Deshpande K. L. Properties of glutamate dehydrogenase from Bacillus subtilis. Biochem Biophys Res Commun. 1979 Jun 13;88(3):761–767. doi: 10.1016/0006-291x(79)91473-6. [DOI] [PubMed] [Google Scholar]
  11. Kane J. F., Stenmark S. L., Calhoun D. H., Jensen R. A. Metabolic interlock. The role of the subordinate type of enzyme in the regulation of a complex pathway. J Biol Chem. 1971 Jul 10;246(13):4308–4316. [PubMed] [Google Scholar]
  12. Kimura K., Miyakawa A., Imai T., Sasakawa T. Glutamate dehydrogenase from Bacillus subtilis PCI 219. I. Purification and properties. J Biochem. 1977 Feb;81(2):467–476. doi: 10.1093/oxfordjournals.jbchem.a131480. [DOI] [PubMed] [Google Scholar]
  13. Lopez J. M., Marks C. L., Freese E. The decrease of guanine nucleotides initiates sporulation of Bacillus subtilis. Biochim Biophys Acta. 1979 Oct 4;587(2):238–252. doi: 10.1016/0304-4165(79)90357-x. [DOI] [PubMed] [Google Scholar]
  14. Pateman J. A. Regulation of synthesis of glutamate dehydrogenase and glutamine synthetase in micro-organisms. Biochem J. 1969 Dec;115(4):769–775. doi: 10.1042/bj1150769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Phibbs P. V., Jr, Bernlohr R. W. Purification, properties, and regulation of glutamic dehydrogenase of Bacillus licheniformis. J Bacteriol. 1971 May;106(2):375–385. doi: 10.1128/jb.106.2.375-385.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sterlini J. M., Mandelstam J. Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochem J. 1969 Jun;113(1):29–37. doi: 10.1042/bj1130029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vasantha N., Freese E. Enzyme changes during Bacillus subtilis sporulation caused by deprivation of guanine nucleotides. J Bacteriol. 1980 Dec;144(3):1119–1125. doi: 10.1128/jb.144.3.1119-1125.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES