Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Mar;149(3):1175–1177. doi: 10.1128/jb.149.3.1175-1177.1982

The B66.0 protein of Escherichia coli is the product of the dnaK+ gene.

C Georgopoulos, K Tilly, D Drahos, R Hendrix
PMCID: PMC216516  PMID: 7037741

Abstract

B66.0 is one of the most abundant proteins of Escherichia coli. Its relative rate of synthesis is highly regulated depending on temperature and the growth rate of the culture. We identified the B66.0 protein to be the dnaK+ structural gene product since dnaK756 mutant bacteria synthesized a B66.0 protein with a more acidic isoelectric point.

Full text

PDF
1175

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Georgiou M., Georgopoulos C. P., Eisen H. An analysis of the Tro phenotype of bacteriophage lambda. Virology. 1979 Apr 15;94(1):38–54. doi: 10.1016/0042-6822(79)90436-7. [DOI] [PubMed] [Google Scholar]
  2. Georgopoulos C. P. A new bacterial gene (groPC) which affects lambda DNA replication. Mol Gen Genet. 1977 Feb 28;151(1):35–39. doi: 10.1007/BF00446910. [DOI] [PubMed] [Google Scholar]
  3. Georgopoulos C. P., Lam B., Lundquist-Heil A., Rudolph C. F., Yochem J., Feiss M. Identification of the C. coli dnaK (groPC756) gene product. Mol Gen Genet. 1979 May 4;172(2):143–149. doi: 10.1007/BF00268275. [DOI] [PubMed] [Google Scholar]
  4. Neidhardt F. C., Phillips T. A., VanBogelen R. A., Smith M. W., Georgalis Y., Subramanian A. R. Identity of the B56.5 protein, the A-protein, and the groE gene product of Escherichia coli. J Bacteriol. 1981 Jan;145(1):513–520. doi: 10.1128/jb.145.1.513-520.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Neidhardt F. C., VanBogelen R. A. Positive regulatory gene for temperature-controlled proteins in Escherichia coli. Biochem Biophys Res Commun. 1981 May 29;100(2):894–900. doi: 10.1016/s0006-291x(81)80257-4. [DOI] [PubMed] [Google Scholar]
  6. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  7. Pedersen S., Bloch P. L., Reeh S., Neidhardt F. C. Patterns of protein synthesis in E. coli: a catalog of the amount of 140 individual proteins at different growth rates. Cell. 1978 May;14(1):179–190. doi: 10.1016/0092-8674(78)90312-4. [DOI] [PubMed] [Google Scholar]
  8. Tilly K., Murialdo H., Georgopoulos C. Identification of a second Escherichia coli groE gene whose product is necessary for bacteriophage morphogenesis. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1629–1633. doi: 10.1073/pnas.78.3.1629. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES