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. 1982 Feb;149(2):567–575. doi: 10.1128/jb.149.2.567-575.1982

Effects of Growth Temperature on Fatty Acid and Alk-1-Enyl Group Compositions of Veillonella parvula and Megasphaera elsdenii Phospholipids

Norah C Johnston 1, Howard Goldfine 1
PMCID: PMC216544  PMID: 7056696

Abstract

Veillonella parvula ATCC 10790, an anaerobic gram-negative coccus, contains diacyl and alk-1-enyl acyl (plasmalogen) forms of phosphatidylethanolamine and phosphatidylserine. We studied the effect of growth temperature on the lipid composition of this strain. There was a small increase in the phosphatidylethanolamine content but no change in the content of plasmalogens at the lower growth temperatures tested. The total acyl chains and the plasmalogen acyl chains contained between 73 and 80% mono-unsaturated fatty acids at all growth temperatures. The plasmalogen alk-1-enyl chains were significantly more unsaturated in cells grown at 30 and 25°C than in cells grown at 37°C. Differential scanning calorimetry of the hydrated phospholipids showed lower phase transition temperatures for the lipids from the cells grown at the lower temperatures. In Megasphaera elsdenii lipids, which are similar in composition to the lipids of V. parvula, the proportion of phosphatidylethanolamine also increased slightly at lower growth temperatures, with no significant change in the content of plasmalogens. M. elsdenii contained cyclopropane fatty acyl and alk-1-enyl chains in addition to the mono-unsaturated and saturated chains previously reported. As cells entered the stationary phase of growth at 30 and 42.5°C, there was a reciprocal increase in the proportion of cyclopropane acyl chains and decrease in the unsaturated moieties. The total proportion of cyclopropane and unsaturated acyl and alk-1-enyl chains was more than 65% at all growth temperatures studied, and there was no discernible increase in the sum of these moieties at the lower growth temperatures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  2. BAUMANN N. A., HAGEN P. O., GOLDFINE H. PHOSPHOLIPIDS OF CLOSTRIDIUM BUTYRICUM. STUDIES ON PLASMALOGEN COMPOSITION AND BIOSYNTHESIS. J Biol Chem. 1965 Apr;240:1559–1567. [PubMed] [Google Scholar]
  3. Cronan J. E., Jr Thermal regulation of the membrane lipid composition of Escherichia coli. Evidence for the direct control of fatty acid synthesis. J Biol Chem. 1975 Sep 10;250(17):7074–7077. [PubMed] [Google Scholar]
  4. Day J. I., Goldfine H. Partial purification and properties of acyl-CoA reductase from Clostridium butyricum. Arch Biochem Biophys. 1978 Sep;190(1):322–331. doi: 10.1016/0003-9861(78)90282-5. [DOI] [PubMed] [Google Scholar]
  5. GOLDFINE H., BLOCH K. On the origin of unsaturated fatty acids in clostridia. J Biol Chem. 1961 Oct;236:2596–2601. [PubMed] [Google Scholar]
  6. GOLDFINE H. COMPOSITION OF THE ALDEHYDES OF CLOSTRIDIUM BUTYRICUM PLASMALOGENS. CYCLOPROPANE ALDEHYDES. J Biol Chem. 1964 Jul;239:2130–2134. [PubMed] [Google Scholar]
  7. GOTTFRIED E. L., RAPPORT M. M. The biochemistry of plasmalogens. I. Isolation and characterization of phosphatidal choline, a pure native plasmalogen. J Biol Chem. 1962 Feb;237:329–333. [PubMed] [Google Scholar]
  8. Gilbertson J. R., Ferrell W. J., Gelman R. A. Isolation and analysis of free fatty aldehydes from rat, dog, and bovine heart muscle. J Lipid Res. 1967 Jan;8(1):38–45. [PubMed] [Google Scholar]
  9. Goldfine H., Johnston N. C., Phillips M. C. Phase behavior of ether lipids from Clostridium butyricum. Biochemistry. 1981 May 12;20(10):2908–2916. doi: 10.1021/bi00513a030. [DOI] [PubMed] [Google Scholar]
  10. Goldfine H., Khuller G. K., Borie R. P., Silverman B., Selick H., 2nd, Johnston N. C., Vanderkooi J. M., Horwitz A. F. Effects of growth temperature and supplementation with exogenous fatty acids on some physical properties of Clostridium butyricum phospholipids. Biochim Biophys Acta. 1977 Sep 28;488(3):341–352. doi: 10.1016/0005-2760(77)90193-x. [DOI] [PubMed] [Google Scholar]
  11. HOFMANN K., LUCAS R. A., SAX S. M. The chemical nature of the fatty acids of Lactobacillus arabinosus. J Biol Chem. 1952 Apr;195(2):473–485. [PubMed] [Google Scholar]
  12. Hagen P. O., Goldfine H. Phospholipids of Clostridium butyricum. 3. Further studies on the origin of the aldehyde chains of plasmalogens. J Biol Chem. 1967 Dec 25;242(24):5700–5708. [PubMed] [Google Scholar]
  13. Khuller G. K., Goldfine H. Phospholipids of Clostridium butyricum. V. Effects of growth temperature on fatty acid, alk-1-enyl ether group, and phospholipid composition. J Lipid Res. 1974 Sep;15(5):500–507. [PubMed] [Google Scholar]
  14. Khuller G. K., Goldfine H. Replacement of acyl and alk-1-enyl groups in Clostridium butyricum phospholipids by exogenous fatty acids. Biochemistry. 1975 Aug 12;14(16):3642–3647. doi: 10.1021/bi00687a020. [DOI] [PubMed] [Google Scholar]
  15. Knivett V. A., Cullen J. Some factors affecting cyclopropane acid formation in Escherichia coli. Biochem J. 1965 Sep;96(3):771–776. doi: 10.1042/bj0960771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McGarrity J. T., Armstrong J. B. Phase transition behaviour of artificial liposomes composed of phosphatidylcholines acylated with cyclopropane fatty acids. Biochim Biophys Acta. 1981 Jan 22;640(2):544–548. doi: 10.1016/0005-2736(81)90478-8. [DOI] [PubMed] [Google Scholar]
  17. Okuyama H., Yamada K., Kameyama Y., Ikezawa H., Akamatsu Y., Nojima S. Regulation of membrane lipid synthesis in Escherichia coli after shifts in temperature. Biochemistry. 1977 Jun 14;16(12):2668–2673. doi: 10.1021/bi00631a013. [DOI] [PubMed] [Google Scholar]
  18. Roots B. I., Johnston P. V. Plasmalogens of the nervous system and environmental temperature. Comp Biochem Physiol. 1968 Aug;26(2):553–560. doi: 10.1016/0010-406x(68)90648-8. [DOI] [PubMed] [Google Scholar]
  19. Roots B. I. Phospholipids of goldfish (Carassius auratus L.) brain: the influence of environmental temperature. Comp Biochem Physiol. 1968 May;25(2):457–466. doi: 10.1016/0010-406x(68)90354-x. [DOI] [PubMed] [Google Scholar]
  20. Taylor F. R., Cronan J. E., Jr Cyclopropane fatty acid synthase of Escherichia coli. Stabilization, purification, and interaction with phospholipid vesicles. Biochemistry. 1979 Jul 24;18(15):3292–3300. doi: 10.1021/bi00582a015. [DOI] [PubMed] [Google Scholar]
  21. Van Golde L. M., Akkermans-Kruyswijk J., Franklin-Klein W., Lankhorst A., Prins R. A. Accumulation of phosphatidylserine in strictly anaerobic lactate fermenting bacteria. FEBS Lett. 1975 Apr 15;53(1):57–60. doi: 10.1016/0014-5793(75)80681-8. [DOI] [PubMed] [Google Scholar]
  22. Verkley A. J., Ververgaert P. H., Prins R. A., van Golde L. M. Lipid-phase transitions of the strictly anaerobic bacteria Veillonella parvula and Anaerovibrio lipolytica. J Bacteriol. 1975 Dec;124(3):1522–1528. doi: 10.1128/jb.124.3.1522-1528.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. van Golde L. M., Prins R. A., Franklin-Klein W., Akkermans-Kruyswijk J. Phosphatidylserine and its plasmalogen analogue as major lipid constituents in Megasphaera elsdenii. Biochim Biophys Acta. 1973 Dec 20;326(3):314–324. doi: 10.1016/0005-2760(73)90133-1. [DOI] [PubMed] [Google Scholar]

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