Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Feb;149(2):768–770. doi: 10.1128/jb.149.2.768-770.1982

Extracellular exonuclease as a stage 0 biochemical marker in Bacillus subtilis sporulation.

W F Burke Jr, B S Slinker
PMCID: PMC216570  PMID: 6276370

Abstract

Calcium-dependent exonuclease activity was produced by sporulating cells of Bacillus subtilis 168. Nuclease activity was released into the culture medium at approximately the same time as sporulation proteases, and production of these enzymes was tied to DNA replication. Results suggest that nuclease production is a function of the spo0H locus.

Full text

PDF
770

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akrigg A., Mandelstam J. Extracellular manganese-stimulated deoxyribonuclease as a marker event in sporulation of Bacillus subtilis. Biochem J. 1978 Apr 15;172(1):63–67. doi: 10.1042/bj1720063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cheng Y. S., Aronson A. I. Characterization and function of intracellular proteases in sporulating Bacillus cereus. Arch Microbiol. 1977 Oct 24;115(1):61–66. doi: 10.1007/BF00427846. [DOI] [PubMed] [Google Scholar]
  3. Dancer B. N., Mandelstam J. Production and possible function of serine protease during sporulation of Bacillus subtilis. J Bacteriol. 1975 Feb;121(2):406–410. doi: 10.1128/jb.121.2.406-410.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Glenn A. R., Mandelstam J. Sporulation in Bacillus subtilis 168. Comparison of alkaline phosphatase from sporulating and vegetative cells. Biochem J. 1971 Jun;123(2):129–138. doi: 10.1042/bj1230129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kerr I. M., Pratt E. A., Lehman I. R. Exonucleolytic degradation of high-molecular-weight DNA and RNA to nucleoside 3'-phosphates by a nuclease from B. subtilis. Biochem Biophys Res Commun. 1965 Jul 12;20(2):154–162. doi: 10.1016/0006-291x(65)90339-6. [DOI] [PubMed] [Google Scholar]
  6. Leighton T. J., Doi R. H. The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis. J Biol Chem. 1971 May 25;246(10):3189–3195. [PubMed] [Google Scholar]
  7. Mandelstam J., Sterlini J. M., Kay D. Sporulation in Bacillus subtilis. Effect of medium on the form of chromosome replication and on initiation to sporulation in Bacillus subtilis. Biochem J. 1971 Nov;125(2):635–641. doi: 10.1042/bj1250635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Millet J. Characterization of proteinases excreted by Bacillus subtilis Marburg strain during sporulation. J Appl Bacteriol. 1970 Mar;33(1):207–219. doi: 10.1111/j.1365-2672.1970.tb05245.x. [DOI] [PubMed] [Google Scholar]
  9. NAKAI M., MINAMI Z., YAMAZAKI T., TSUGITA A. STUDIES ON THE NUCLEASES OF A STRAIN OF BACILLUS SUBTILIS. J Biochem. 1965 Jan;57:96–99. doi: 10.1093/oxfordjournals.jbchem.a128063. [DOI] [PubMed] [Google Scholar]
  10. Okazaki R., Okazaki T., Sakabe K. An extracellular nuclease of Bacillus subtilis: some novel properties as a DNA exonuclease. Biochem Biophys Res Commun. 1966 Mar 22;22(6):611–619. doi: 10.1016/0006-291x(66)90190-2. [DOI] [PubMed] [Google Scholar]
  11. Piggot P. J., Coote J. G. Genetic aspects of bacterial endospore formation. Bacteriol Rev. 1976 Dec;40(4):908–962. doi: 10.1128/br.40.4.908-962.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ryter A., Schaeffer P., Ionesco H. Classification cytologique, par leur stade de blocage, des mutants de sporulation de Bacillus subtilis Marburg. Ann Inst Pasteur (Paris) 1966 Mar;110(3):305–315. [PubMed] [Google Scholar]
  13. Sterlini J. M., Mandelstam J. Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochem J. 1969 Jun;113(1):29–37. doi: 10.1042/bj1130029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Warren S. C. Sporulation in Bacillus subtilis. Biochemical changes. Biochem J. 1968 Oct;109(5):811–818. doi: 10.1042/bj1090811. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES