Abstract
Several mono-, di, tetra-, and polysaccharides were screened for their ability to induced cellulase production by the tetrapolar hymenomycete Schizophyllum commune. Out of 21 carbohydrates screened, 4 (thiocellobiose, carboxymethylcellulose, cellobiose, and xylan) induced all three enzymes tested (carboxymethylcellulase, beta-glucosidase, and xylanase). The inducing effect increased with rising concentrations of the inducers up to a certain value, beyond which there was either a leveling off or a decrease of the enzymatic activities. The most powerful inducer, thiocellobiose, showed the highest activity at 0.5 mM. Cellobiose, carboxymethylcellulose, and xylan showed their highest activities at 1 mM and 1%, respectively. Surprisingly, sophorose did not enhance enzyme production. The enzymatic activities were monitored over a period of 24 h. Thiocelloboise elicited a response immediately after incubation, but with all other inducers there was a latency period before their effect could be measured. High-performance liquid chromatography showed no hydrolysis of thiocellobiose when incubated in the presence of S. commune extracellular enzymes.
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- Biely P., Krátký Z., Vrsanská M., Urmanicová D. Induction and inducers of endo-1,4-beta-xylanase in the yeast Cryptococcus albidus. Eur J Biochem. 1980;108(1):323–329. doi: 10.1111/j.1432-1033.1980.tb04726.x. [DOI] [PubMed] [Google Scholar]
- Boos W., Schaedel P., Wallenfels K. Untersuchungen zur Induktion der Lac-Enzyme. 1. Induktionswirkung und Permeation. Eur J Biochem. 1967 Jun;1(4):382–394. doi: 10.1111/j.1432-1033.1967.tb00086.x. [DOI] [PubMed] [Google Scholar]
- Kanda T., Wakabayashi K., Nisizawa K. Purification and properties of an endo-cellulase of avicelase type from Irpex lacteus (Polyporus tulipiferae). J Biochem. 1976 May;79(5):977–988. doi: 10.1093/oxfordjournals.jbchem.a131165. [DOI] [PubMed] [Google Scholar]
- MANDELS M., REESE E. T. Induction of cellulase in fungi by cellobiose. J Bacteriol. 1960 Jun;79:816–826. doi: 10.1128/jb.79.6.816-826.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mandels M. Microbial sources of cellulase. Biotechnol Bioeng Symp. 1975;(5):81–105. [PubMed] [Google Scholar]
- Paice M. G., Jurasek L., Carpenter M. R., Smillie L. B. Production, characterization, and partial amino acid sequence of xylanase A from Schizophyllum commune. Appl Environ Microbiol. 1978 Dec;36(6):802–808. doi: 10.1128/aem.36.6.802-808.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shewale J. G., Sadana J. C. Cellulase and beta-glucosidase production by a basidiomycete species. Can J Microbiol. 1978 Oct;24(10):1204–1216. doi: 10.1139/m78-195. [DOI] [PubMed] [Google Scholar]
- Sternberg D., Dorval S. Cellulase production and ammonia metabolism in Trichoderma reesei on high levels of cellulose. Biotechnol Bioeng. 1979 Feb;21(2):181–191. doi: 10.1002/bit.260210205. [DOI] [PubMed] [Google Scholar]
- Sternberg D., Mandels G. R. Induction of cellulolytic enzymes in Trichoderma reesei by sophorose. J Bacteriol. 1979 Sep;139(3):761–769. doi: 10.1128/jb.139.3.761-769.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]