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. 1979 Dec;140(3):944–948. doi: 10.1128/jb.140.3.944-948.1979

Carotenoids acts as reinforcers of the Acholeplasma laidlawii lipid bilayer.

S Rottem, O Markowitz
PMCID: PMC216737  PMID: 533770

Abstract

Acholeplasma laidlawii cells grown with oleic acid produced much more colored carotenoids than did cells grown with elaidic acid. The amount of carotenoids was decreased 80 to 90% by growing the cells with 0.05 M propionate, resulting in a marked increase in the mobility of both 5-doxylstearate and 12-doxylstearate incorporated into the membranes. The fatty acid composition of the propionate-grown cells differed from that of cells grown without propionate by containing odd-numbered rather than even-numbered saturated fatty acids, but the ratios of saturated to unsaturated fatty acids were the same. To determine whether the carotenoids are the cause for the restricted mobility in the membranes, the carotenoids were selectively removed from A. laidlawii membranes by incubating the membranes with phosphatidylcholine vesicles. The carotenoid-depleted membranes showed an increase in the mobility of the hydrocarbon chains of the spin-labeled fatty acids. Furthermore, the incorporation of carotenoids into artificial membrane vesicles restricted the mobility of the hydrocarbon chain. Our results support the notion that the carotenoids in A. laidlawii act as a rigid insert reinforcing the membrane bilayer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Bruckdorfer K. R., Edwards P. A., Green C. Properties of aqueous dispersions of phospholipid and cholesterol. Eur J Biochem. 1968 May;4(4):506–511. doi: 10.1111/j.1432-1033.1968.tb00241.x. [DOI] [PubMed] [Google Scholar]
  3. Henry S. A., Keith A. D. Membrane properties of saturated fatty acid mutants of yeast revealed by spin labels. Chem Phys Lipids. 1971 Dec;7(4):245–265. doi: 10.1016/0009-3084(71)90004-1. [DOI] [PubMed] [Google Scholar]
  4. Huang L., Haug A. Regulation of membrane lipid fluidity in Acholeplasma laidlawii: effect of carotenoid pigment content. Biochim Biophys Acta. 1974 Jun 29;352(3):361–370. doi: 10.1016/0005-2736(74)90228-4. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Melchior D. L., Steim J. M. Control of fatty acid composition of Acholeplasma laidlawii membranes. Biochim Biophys Acta. 1977 Apr 1;466(1):148–159. doi: 10.1016/0005-2736(77)90215-2. [DOI] [PubMed] [Google Scholar]
  7. RAZIN S. OSMOTIC LYSIS OF MYCOPLASMA. J Gen Microbiol. 1963 Dec;33:471–475. doi: 10.1099/00221287-33-3-471. [DOI] [PubMed] [Google Scholar]
  8. Razin S., Rottem S. Role of carotenoids and cholesterol in the growth of Mycoplasma laidlawii. J Bacteriol. 1967 Mar;93(3):1181–1182. doi: 10.1128/jb.93.3.1181-1182.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Razin S. Structure and function in mycoplasma. Annu Rev Microbiol. 1969;23:317–356. doi: 10.1146/annurev.mi.23.100169.001533. [DOI] [PubMed] [Google Scholar]
  10. Rohmer M., Bouvier P., Ourisson G. Molecular evolution of biomembranes: structural equivalents and phylogenetic precursors of sterols. Proc Natl Acad Sci U S A. 1979 Feb;76(2):847–851. doi: 10.1073/pnas.76.2.847. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rottem S., Hubbell W. L., Hayflick L., McConnell H. M. Motion of fatty acid spin labels in the plasma membrane of mycoplasma. Biochim Biophys Acta. 1970;219(1):104–113. doi: 10.1016/0005-2736(70)90065-9. [DOI] [PubMed] [Google Scholar]
  12. Rottem S., Razin S. Membrane lipids of Mycoplasma hominis. J Bacteriol. 1973 Feb;113(2):565–571. doi: 10.1128/jb.113.2.565-571.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SMITH P. F. THE CAROTENOID PIGMENTS OF MYCOPLASMA. J Gen Microbiol. 1963 Sep;32:307–319. doi: 10.1099/00221287-32-3-307. [DOI] [PubMed] [Google Scholar]
  14. Saito Y., Silvius J. R., McElhaney N. Membrane lipid biosynthesis in Acholeplasma laidlawii B: de novo biosynthesis of saturated fatty acids by growing cells. J Bacteriol. 1977 Nov;132(2):497–504. doi: 10.1128/jb.132.2.497-504.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Silvius J. R., Saito Y., McElhaney R. N. Membrane lipid biosynthesis in Acholeplasma laidlawii B. Investigations into the in vivo regulation of the quantity and hydrocarbon chain lengths of de novo biosynthesized fatty aicds in response to exogenously supplied fatty acids. Arch Biochem Biophys. 1977 Aug;182(2):455–464. doi: 10.1016/0003-9861(77)90526-4. [DOI] [PubMed] [Google Scholar]
  16. Smith P. F., Henrikson C. V. Growth inhibition of Mycoplasma by inhibitors of polyterpene biosynthesis and its reversal by cholesterol. J Bacteriol. 1966 May;91(5):1854–1858. doi: 10.1128/jb.91.5.1854-1858.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tully J. G., Razin S. Physiological and serological comparisons among strains of Mycoplasma granularum and Mycoplasma laidlawii. J Bacteriol. 1968 May;95(5):1504–1512. doi: 10.1128/jb.95.5.1504-1512.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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