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. 1979 Jul;139(1):115–119. doi: 10.1128/jb.139.1.115-119.1979

Effect of carbamoyl phosphate on nitrogenase in Anabaena cylindrica Lemm.

A C Lawrie
PMCID: PMC216834  PMID: 110775

Abstract

Carbamoyl phosphate inhibited acetylene reduction by whole cells and cell-free extracts of Anabaena cylindrica. Higher levels of both endogenous carbamoyl phosphate and carbamoyl phosphate synthase activity were present in NH4+-grown cells (in which acetylene reduction was absent) than in N2-grown cells (in which acetylene reduction was present). However, inhibition of acetylene reduction was observed also with cyanate, the main initial decomposition product under the conditions used. It is concluded that carbamoyl phosphate or one of its metabolites may act as a physiological regulator of both nitrogenase activity and synthesis, but caution must be used in interpreting effects observed several hours after the addition of carbamoyl phosphate, because the effects may be due to cyanate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLEN C. M., Jr, JONES M. E. DECOMPOSITION OF CARBAMYLPHOSPHATE IN AQUEOUS SOLUTIONS. Biochemistry. 1964 Sep;3:1238–1247. doi: 10.1021/bi00897a010. [DOI] [PubMed] [Google Scholar]
  2. Allen M. B., Arnon D. I. Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm. Plant Physiol. 1955 Jul;30(4):366–372. doi: 10.1104/pp.30.4.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bone D. H. Kinetics of synthesis of nitrogenase in batch and continuous culture of Anabaena flos-aquae. Arch Mikrobiol. 1971;80(3):242–251. doi: 10.1007/BF00410125. [DOI] [PubMed] [Google Scholar]
  4. Bone D. H. The influence of canavanine, oxygen, and urea on the steady-state levels of nitrogenase in Anabaena flos-aquae. Arch Mikrobiol. 1972;86(1):13–24. doi: 10.1007/BF00412396. [DOI] [PubMed] [Google Scholar]
  5. CARNAHAN J. E., MORTENSON L. E., MOWER H. F., CASTLE J. E. Nitrogen fixation in cell-free extracts of Clostridium pasteurianum. Biochim Biophys Acta. 1960 Nov 18;44:520–535. doi: 10.1016/0006-3002(60)91606-1. [DOI] [PubMed] [Google Scholar]
  6. Daesch G., Mortenson L. E. Effect of ammonia on the synthesis and function of the N 2 -fixing enzyme system in Clostridium pasteurianum. J Bacteriol. 1972 Apr;110(1):103–109. doi: 10.1128/jb.110.1.103-109.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Elliott B. B., Mortenson L. E. Regulation of molybdate transport by Clostridium pasteurianum. J Bacteriol. 1976 Aug;127(2):770–779. doi: 10.1128/jb.127.2.770-779.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Haystead A., Robinson R., Stewart W. D. Nitrogenase activity in extracts of heterocystous and non-heterocystous blue-green algae. Arch Mikrobiol. 1970;74(3):235–243. doi: 10.1007/BF00408884. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Meeks J. C., Wolk C. P., Thomas J., Lockau W., Shaffer P. W., Austin S. M., Chien W. S., Galonsky A. The pathways of assimilation of 13NH4+ by the cyanobacterium, Anabaena cylindrica. J Biol Chem. 1977 Nov 10;252(21):7894–7900. [PubMed] [Google Scholar]
  11. Seto B. L., Mortenson L. E. Mechanism of carbamyl phosphate inhibition of nitrogenase of Clostridium pasteurianum. J Bacteriol. 1974 Feb;117(2):805–812. doi: 10.1128/jb.117.2.805-812.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Seto B., Mortenson L. E. Effect of carbamyl phosphate on the regulation of nitrogenase in Clostridium pasteurianum. Biochem Biophys Res Commun. 1973 Jul 17;53(2):419–423. doi: 10.1016/0006-291x(73)90678-5. [DOI] [PubMed] [Google Scholar]
  13. Simon R. D. Cyanophycin Granules from the Blue-Green Alga Anabaena cylindrica: A Reserve Material Consisting of Copolymers of Aspartic Acid and Arginine. Proc Natl Acad Sci U S A. 1971 Feb;68(2):265–267. doi: 10.1073/pnas.68.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stewart W. D., Rowell P. Effects of L-methionine-DL-sulphoximine on the assimilation of newly fixed NH3, acetylene reduction and heterocyst production in Anabaena cylindrica. Biochem Biophys Res Commun. 1975 Aug 4;65(3):846–856. doi: 10.1016/s0006-291x(75)80463-3. [DOI] [PubMed] [Google Scholar]
  15. Tempest D. W., Meers J. L., Brown C. M. Influence of environment on the content and composition of microbial free amino acid pools. J Gen Microbiol. 1970 Dec;64(2):171–185. doi: 10.1099/00221287-64-2-171. [DOI] [PubMed] [Google Scholar]
  16. Williams L. G., Bernhardt S. A., Davis R. H. Evidence for two discrete carbamyl phosphate pools in Neurospora. J Biol Chem. 1971 Feb 25;246(4):973–978. [PubMed] [Google Scholar]
  17. Wolk C. P., Thomas J., Shaffer P. W., Austin S. M., Galonsky A. Pathway of nitrogen metabolism after fixation of 13N-labeled nitrogen gas by the cyanobacterium, Anabaena cylindrica. J Biol Chem. 1976 Aug 25;251(16):5027–5034. [PubMed] [Google Scholar]

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