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. 1979 Jul;139(1):311–315. doi: 10.1128/jb.139.1.311-315.1979

Isolation and characterization of a mutant ColE1 plasmid that allows constitutive colicin E1 synthesis.

H Ohkubo, K Shimada, Y Takagi
PMCID: PMC216862  PMID: 378956

Abstract

It has been possible to isolate a ColE1 mutant which synthesizes colicin E1 constitutively. This result shows that there must be a gene(s) responsible for the regulation of colicin E1 synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barth P. T., Grinter N. J. Map of plasmid RP4 derived by insertion of transposon C. J Mol Biol. 1977 Jul 5;113(3):455–474. doi: 10.1016/0022-2836(77)90233-9. [DOI] [PubMed] [Google Scholar]
  3. Dougan G., Sherratt D. The transposon Tn1 as a probe for studying ColE1 structure and function. Mol Gen Genet. 1977 Mar 7;151(2):151–160. doi: 10.1007/BF00338689. [DOI] [PubMed] [Google Scholar]
  4. Fukumaki Y., Shimada K., Takagi Y. Expression of a bacterial gene for guanine synthesis inserted into colicin E1 factor by an in vitro recombination. Biochim Biophys Acta. 1977 Sep 6;478(1):90–98. doi: 10.1016/0005-2787(77)90247-7. [DOI] [PubMed] [Google Scholar]
  5. Herschman H. R., Helinski D. R. Comparative study of the events associated with colicin induction. J Bacteriol. 1967 Sep;94(3):691–699. doi: 10.1128/jb.94.3.691-699.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hershfield V., Boyer H. W., Chow L., Helinski D. R. Characterization of a mini-ColC1 plasmid. J Bacteriol. 1976 Apr;126(1):447–453. doi: 10.1128/jb.126.1.447-453.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Howard-Flanders P., Theriot L. Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics. 1966 Jun;53(6):1137–1150. doi: 10.1093/genetics/53.6.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Inselburg J. Isolation, mapping, and examination of effects of TnA insertions in ColE1 plasmids. J Bacteriol. 1977 Jan;129(1):482–491. doi: 10.1128/jb.129.1.482-491.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Inselburg J. Studies of colicin E1 plasmid functions by analysis of deletions and TnA insertions of the plasmid. J Bacteriol. 1977 Oct;132(1):332–340. doi: 10.1128/jb.132.1.332-340.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kennedy C. K. Induction of colicin production by high temperature or inhibition of protein synthesis. J Bacteriol. 1971 Oct;108(1):10–19. doi: 10.1128/jb.108.1.10-19.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kool A. J., Nijkamp H. J. Isolation and characterization of a copy mutant of the bacteriocinogenic plasmid Clo DF13. J Bacteriol. 1974 Nov;120(2):569–578. doi: 10.1128/jb.120.2.569-578.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Luria S. E. Phage, colicins, and macroregulatory phenomena. Science. 1970 Jun 5;168(3936):1166–1170. doi: 10.1126/science.168.3936.1166. [DOI] [PubMed] [Google Scholar]
  14. Maeda S., Shimada K., Takagi Y. Colicinogenic mutant of ColE1 plasmid that fails to confer immunity to colicin E1. Gene. 1978 Feb;3(1):1–7. doi: 10.1016/0378-1119(78)90002-1. [DOI] [PubMed] [Google Scholar]
  15. Matsubara K., Takagi Y., Mukai T. In vitro construction of different oligomeric forms of lambdadv DNA and studies of their transforming activities. J Virol. 1975 Sep;16(3):479–485. doi: 10.1128/jvi.16.3.479-485.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. OZEKI H., STOCKER B. A., DE MARGERIE H. Production of colicine by single bacteria. Nature. 1959 Aug 1;184:337–339. doi: 10.1038/184337a0. [DOI] [PubMed] [Google Scholar]
  17. Sargent M. G. Rapid fixed-time assay for penicillinase. J Bacteriol. 1968 Apr;95(4):1493–1494. doi: 10.1128/jb.95.4.1493-1494.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sato K., Nishimune Y., Sato M., Numich R., Matsushiro A. Suppressor-sensitive mutants of coliphage phi-80. Virology. 1968 Apr;34(4):637–649. [PubMed] [Google Scholar]
  19. Shimada K., Nakamura T., Maeda S., Takagi Y. Generation of small plasmids from colicin E1 factor carrying genes for galactose utilization and ampicillin resistance,. Biochem Biophys Res Commun. 1977 Apr 11;75(3):659–666. doi: 10.1016/0006-291x(77)91523-6. [DOI] [PubMed] [Google Scholar]
  20. So M., Gill R., Falkow S. The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA. Mol Gen Genet. 1975 Dec 30;142(3):239–249. doi: 10.1007/BF00425649. [DOI] [PubMed] [Google Scholar]
  21. Uhlin B. E., Nordström K. R plasmid gene dosage effects in Escherichia coli K-12: copy mutants of the R plasmic R1drd-19. Plasmid. 1977 Nov;1(1):1–7. doi: 10.1016/0147-619x(77)90003-8. [DOI] [PubMed] [Google Scholar]

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