Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1979 Aug;139(2):404–410. doi: 10.1128/jb.139.2.404-410.1979

Specialized lambda transducing bacteriophage which carries hisS, the structural gene for histidyl-transfer ribonucleic acid synthetase.

S E Fishman, K R Kerchief, J Parker
PMCID: PMC216883  PMID: 378969

Abstract

A number of specialized lambda transducing bacteriophages which carry the Escherichia coli gene guaB were isolated from E. coli. One of these bacteriophages, lambda cI857 Sam7 d guaB-2, also carries hisS, the structural gene for histidyl-transfer ribonucleic acid synthetase (EC 6.1.1.21). Histidyl-transfer ribonucleic acid synthetase activities in induced and uninduced lysogens carrying lambda d guaB-2 indicate that the phage carries the entire structural gene and that the gene is under the control of an E. coli promoter. These conclusions were confirmed by the in vivo production of a protein encoded by the phage which comigrates with authentic histidyl-transfer ribonucleic acid synthetase on two-dimensional polyacrylamide gels.

Full text

PDF
405

Images in this article

408Image
on p.408

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson E. H. Growth Requirements of Virus-Resistant Mutants of Escherichia Coli Strain "B". Proc Natl Acad Sci U S A. 1946 May;32(5):120–128. doi: 10.1073/pnas.32.5.120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cassio D., Waller J. -P. Purification and properties of methionyl-tRNA synthetase from E. coli K 12 carrying the F32 episome. FEBS Lett. 1971 Feb 9;12(6):309–312. doi: 10.1016/0014-5793(71)80002-9. [DOI] [PubMed] [Google Scholar]
  3. Folk W. R., Berg P. Duplication of the structural gene for glycyl-transfer RNA synthetase in Escherichia coli. J Mol Biol. 1971 Jun 14;58(2):595–610. doi: 10.1016/0022-2836(71)90374-3. [DOI] [PubMed] [Google Scholar]
  4. Friesen J. D., Parker J., Watson R. J., Bendiak D., Reeh S. V., Pedersen S., Fiil N. P. A transducing bacteriophage lambda carrying the structural gene for elongation factor Ts. Mol Gen Genet. 1976 Oct 18;148(1):93–98. doi: 10.1007/BF00268549. [DOI] [PubMed] [Google Scholar]
  5. Friesen J. D., Parker J., Watson R. J., Fiil N. P., Pedersen S. Isolation of transducing phage carrying rps T, the structural gene for ribosomal protein S20. Mol Gen Genet. 1976 Feb 27;144(1):115–118. doi: 10.1007/BF00277314. [DOI] [PubMed] [Google Scholar]
  6. Hennecke H., Böck A., Thomale J., Nass G. Threonyl-transfer ribonucleic acid synthetase from Escherichia coli: subunit structure and genetic analysis of the structural gene by means of a mutated enzyme and of a specialized transducing lambda bacteriophage. J Bacteriol. 1977 Sep;131(3):943–950. doi: 10.1128/jb.131.3.943-950.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jaskunas S. R., Lindahl L., Nomura M. Specialized transducing phages for ribosomal protein genes of Escherichia coli. Proc Natl Acad Sci U S A. 1975 Jan;72(1):6–10. doi: 10.1073/pnas.72.1.6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kalousek F., Konigsberg W. H. Purification and characterization of histidyl transfer ribonucleic acid synthetase of Escherichia coli. Biochemistry. 1974 Feb 26;13(5):999–1006. doi: 10.1021/bi00702a026. [DOI] [PubMed] [Google Scholar]
  9. Kirschbaum J. B., Konrad E. B. Isolation of a specialized lambda transducing bacteriophage carrying the beta subunit gene for Escherichia coli ribonucleic acid polymerase. J Bacteriol. 1973 Nov;116(2):517–526. doi: 10.1128/jb.116.2.517-526.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Lambden P. R., Drabble W. T. The gua operon of Escherichia coli K-12: evidence for polarity from guaB to guaA. J Bacteriol. 1973 Sep;115(3):992–1002. doi: 10.1128/jb.115.3.992-1002.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lindahl L., Jaskunas S. R., Dennis P. P., Nomura M. Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2743–2747. doi: 10.1073/pnas.72.7.2743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lund E., Dahlberg J. E., Lindahl L., Jaskunas S. R., Dennis P. P., Nomura M. Transfer RNA genes between 16S and 23S rRNA genes in rRNA transcription units of E. coli. Cell. 1976 Feb;7(2):165–177. doi: 10.1016/0092-8674(76)90016-7. [DOI] [PubMed] [Google Scholar]
  15. McGinnis E., Williams L. S. Regulation of histidyl-transfer ribonucleic acid synthetase formation in a histidyl-transfer ribonucleic acid synthetase mutant of Salmonella typhimurium. J Bacteriol. 1972 Sep;111(3):739–744. doi: 10.1128/jb.111.3.739-744.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McGinnis E., Williams L. S. Role of histidine transfer ribonucleic acid in regulation of synthesis of histidyl-transfer ribonucleic acid synthetase of Salmonella typhimurium. J Bacteriol. 1972 Feb;109(2):505–511. doi: 10.1128/jb.109.2.505-511.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Morgan E. A., Nomura M. Deletion analysis of the expression of rRNA genes and associated tRNA genes carried by a lambda transducing bacteriophage. J Bacteriol. 1979 Jan;137(1):507–516. doi: 10.1128/jb.137.1.507-516.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Morgan S. D., Söll D. Regulation of the biosynthesis of aminoacid: tRNA ligases and of tRNA. Prog Nucleic Acid Res Mol Biol. 1978;21:181–207. doi: 10.1016/s0079-6603(08)60270-6. [DOI] [PubMed] [Google Scholar]
  19. Neidhardt F. C., Bloch P. L., Pedersen S., Reeh S. Chemical measurement of steady-state levels of ten aminoacyl-transfer ribonucleic acid synthetases in Escherichia coli. J Bacteriol. 1977 Jan;129(1):378–387. doi: 10.1128/jb.129.1.378-387.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Neihardt F. C., Parker J., McKeever W. G. Function and regulation of aminoacyl-tRNA synthetases in prokaryotic and eukaryotic cells. Annu Rev Microbiol. 1975;29:215–250. doi: 10.1146/annurev.mi.29.100175.001243. [DOI] [PubMed] [Google Scholar]
  21. Nijkamp H. J., Oskamp A. A. Regulation of the biosynthesis of guanosine 5'-monophosphate: evidence for one operon. J Mol Biol. 1968 Jul 14;35(1):103–109. doi: 10.1016/s0022-2836(68)80040-3. [DOI] [PubMed] [Google Scholar]
  22. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  23. Parker J., Fishman S. E. Mapping hisS, the structural gene for histidyl-transfer ribonucleic acid synthetase, in Escherichia coli. J Bacteriol. 1979 Apr;138(1):264–267. doi: 10.1128/jb.138.1.264-267.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Parker J., Flashner M., Mckeever W. G., Neidhardt F. C. Metabolic regulation of the arginyl and valyl transfer ribonucleic acid synthetases in bacteria. J Biol Chem. 1974 Feb 25;249(4):1044–1053. [PubMed] [Google Scholar]
  25. Parker J., Neidhardt F. C. Metabolic regulation of aminoacyl-tRNA synthetase formation in bacteria. Biochem Biophys Res Commun. 1972 Oct 17;49(2):495–501. doi: 10.1016/0006-291x(72)90438-x. [DOI] [PubMed] [Google Scholar]
  26. Parker J., Pollard J. W., Friesen J. D., Stanners C. P. Stuttering: high-level mistranslation in animal and bacterial cells. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1091–1095. doi: 10.1073/pnas.75.3.1091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Pedersen S., Reeh S. V. Analysis of the proteins synthesized in ultraviolet light-irradiated Escherichia coli following infection with the bacteriophages lambdadrifd 18 and lambdadfus-3. Mol Gen Genet. 1976 Mar 30;144(3):339–343. doi: 10.1007/BF00341733. [DOI] [PubMed] [Google Scholar]
  28. Piepersberg A., Hennecke H., Engelhard M., Nass G., Böck A. Cross-reactivity of phenylalanyl-transfer ribonucleic acid ligases from different microorganisms. J Bacteriol. 1975 Dec;124(3):1482–1488. doi: 10.1128/jb.124.3.1482-1488.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Reeh S., Pedersen S., Neidhardt F. C. Transient rates of synthesis of five amionacyl-transfer ribonucleic acid synthetases during a shift-up of Escherichia coli. J Bacteriol. 1977 Feb;129(2):702–706. doi: 10.1128/jb.129.2.702-706.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sakamoto N., Hatfield G. W., Moyed H. S. Physical properties and subunit structure of xanthosine 5'-phosphate aminase. J Biol Chem. 1972 Sep 25;247(18):5880–5887. [PubMed] [Google Scholar]
  31. Schrenk W. J., Weisberg R. A. A simple method for making new transducing lines of coliphage lambda. Mol Gen Genet. 1975;137(2):101–107. doi: 10.1007/BF00341676. [DOI] [PubMed] [Google Scholar]
  32. Shimada K., Weisberg R. A., Gottesman M. E. Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J Mol Biol. 1972 Feb 14;63(3):483–503. doi: 10.1016/0022-2836(72)90443-3. [DOI] [PubMed] [Google Scholar]
  33. Signer E. R. Interaction of prophages at the att80 site with the chromosome of Escherichia coli. J Mol Biol. 1966 Jan;15(1):243–255. doi: 10.1016/s0022-2836(66)80224-3. [DOI] [PubMed] [Google Scholar]
  34. Vales L. D., Chase J. W., Murphy J. B. Orientation of the guanine operon of Escherichia coli K-12 by utilizing strains containing guaB-xse and guaB-upp deletions. J Bacteriol. 1979 Jul;139(1):320–322. doi: 10.1128/jb.139.1.320-322.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Watson R. J., Parker J., Fiil N. P., Flaks J. G., Friesen J. D. New chromosomal location for structural genes of ribosomal proteins. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2765–2769. doi: 10.1073/pnas.72.7.2765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Williams L. S., Neidhardt F. C. Synthesis and inactivation of aminoacyl-transfer RNA synthetases during growth of Escherichia coli. J Mol Biol. 1969 Aug 14;43(3):529–550. doi: 10.1016/0022-2836(69)90357-x. [DOI] [PubMed] [Google Scholar]
  37. Wyche J. H., Ely B., Cebula T. A., Snead M. C., Hartman P. E. Histidyl-transfer ribonucleic acid synthetase in positive control of the histidine operon in Salmonella typhimurium. J Bacteriol. 1974 Feb;117(2):708–716. doi: 10.1128/jb.117.2.708-716.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Yamamoto M., Nomura M. Organization of genes for transcription and translation in the rif region of the Escherichia coli chromosome. J Bacteriol. 1979 Jan;137(1):584–594. doi: 10.1128/jb.137.1.584-594.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Yamamoto M., Strycharz W. A., Nomura M. Identification of genes for elongation factor Ts and ribosomal protein S2 in E. coli. Cell. 1976 May;8(1):129–138. doi: 10.1016/0092-8674(76)90194-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES